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Structured Review

Azenta custom dna array
Determining assembly efficiency by gel electrophoresis, related to step <t>7</t> <t>sgRNA</t> library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb <t>DNA</t> ladder were included for size validation.
Custom Dna Array, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+array/array+custom+dna/pmc12478239-239-8-19
Average 86 stars, based on 1 article reviews
custom dna array - by Bioz Stars, 2026-09
86/100 stars

Images

1) Product Images from "In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models"

Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models

Journal: STAR Protocols

doi: 10.1016/j.xpro.2025.104042

Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.
Figure Legend Snippet: Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

Techniques Used: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Biomarker Discovery

Related Articles

Recombinant:

Article Title: Development and Comparative Evaluation of Endolysosomal Proximity Labeling-Based Proteomic Methods in Human iPSC-Derived Neurons.
Article Snippet: Briefly, 1.5 million cells were seeded onto a 6-well dish for reverse transfection with 42 Lipofectamine Stem (ThermoFisher). .. A ribonucleoprotein particle containing a crRNA targeting the 3’ end 43 of the LAMP1 ORF, tracrRNA, and recombinant Cas9 protein, was co-transfected with a custom DNA 44 plasmid harboring 1-kb homology fragments flanking the APEX gene and a fluorescent selection cassette 45 (Genewiz). ..

Plasmid Preparation:

Article Title: Development and Comparative Evaluation of Endolysosomal Proximity Labeling-Based Proteomic Methods in Human iPSC-Derived Neurons.
Article Snippet: Briefly, 1.5 million cells were seeded onto a 6-well dish for reverse transfection with 42 Lipofectamine Stem (ThermoFisher). .. A ribonucleoprotein particle containing a crRNA targeting the 3’ end 43 of the LAMP1 ORF, tracrRNA, and recombinant Cas9 protein, was co-transfected with a custom DNA 44 plasmid harboring 1-kb homology fragments flanking the APEX gene and a fluorescent selection cassette 45 (Genewiz). ..

Selection:

Article Title: Development and Comparative Evaluation of Endolysosomal Proximity Labeling-Based Proteomic Methods in Human iPSC-Derived Neurons.
Article Snippet: Briefly, 1.5 million cells were seeded onto a 6-well dish for reverse transfection with 42 Lipofectamine Stem (ThermoFisher). .. A ribonucleoprotein particle containing a crRNA targeting the 3’ end 43 of the LAMP1 ORF, tracrRNA, and recombinant Cas9 protein, was co-transfected with a custom DNA 44 plasmid harboring 1-kb homology fragments flanking the APEX gene and a fluorescent selection cassette 45 (Genewiz). ..

DNA Array:

Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models
Article Snippet: Note: The trans-activating CRISPR RNA ( tracr RNA) sequence varies in the pipeline of the Genetic Perurbation Platform (GPP) tool ( https://portals.broadinstitute.org/gpp/public/ , gtttv for the Chen version and gtttt for the Hsu version)., 3. .. Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high-throughput synthesis service (for instance, GeneWiz or GenScript). ..

Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models.
Article Snippet: .. Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high- throughput synthesis service (for instance, GeneWiz or GenScript). ..

High Throughput Screening Assay:

Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models.
Article Snippet: .. Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high- throughput synthesis service (for instance, GeneWiz or GenScript). ..



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Azenta custom dna array
Determining assembly efficiency by gel electrophoresis, related to step <t>7</t> <t>sgRNA</t> library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb <t>DNA</t> ladder were included for size validation.
Custom Dna Array, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+array/array+custom+dna/pmc12478239-239-8-19
Average 86 stars, based on 1 article reviews
custom dna array - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

Journal: STAR Protocols

Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models

doi: 10.1016/j.xpro.2025.104042

Figure Lengend Snippet: Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

Article Snippet: Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high-throughput synthesis service (for instance, GeneWiz or GenScript).

Techniques: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Biomarker Discovery