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cucpt22  (MedChemExpress)


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    Structured Review

    MedChemExpress cucpt22
    Cucpt22, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cucpt22/CU-CPT22/pm32120313-101-44-47
    Average 94 stars, based on 20 article reviews
    cucpt22 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Cholesterol-modified DP7 enhances the effect of individualized cancer immunotherapy based on neoantigens.
    Article Snippet: The protein samples were probed with 250 anti-protease-activated complex subunit 2 (Psme2) (Abcam), anti-calreticulin 251 (Abcam), and anti-antigen peptide transporter 1 (Tap1) (Abcam) antibodies and then 252 incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cell 253 Signaling Technology). .. When pathway inhibitors were used, DCs were pretreated 254 with 50 μM MG-132 (NF-κB inhibitor) (Selleckchem, US) for 1 h, 40 μM ST2825 255 (MyD88 inhibitor) (MCE, US) for 3 h, 150 μM C29 (TLR2 inhibitor) (MCE, US) for 256 1 h, 8 μM CUCPT22 (TLR2 inhibitor) (MCE, US) for 2 h or 1 μM Tak-242 (TLR4 257 inhibitor) (MCE, US) for 30 min. Then, the samples were incubated with 5 μg/ml 258 DP7-C for 2 h and subjected to Western Blot analysis. .. 259 260 T cell proliferation assay 261 To verify the effect of DCs loaded with DP7-C/OVA peptides on T cell proliferation, 262 splenocytes from OT-I and OT-II mice were separated using a lymphocyte isolation 263 solution (Dakewe Biotech Co., Ltd.) and stained with a CFSE kit (Beyotime 264 Biotechnology) according to the manufacturer’s protocol[23].

    Western Blot:

    Article Title: Cholesterol-modified DP7 enhances the effect of individualized cancer immunotherapy based on neoantigens.
    Article Snippet: The protein samples were probed with 250 anti-protease-activated complex subunit 2 (Psme2) (Abcam), anti-calreticulin 251 (Abcam), and anti-antigen peptide transporter 1 (Tap1) (Abcam) antibodies and then 252 incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Cell 253 Signaling Technology). .. When pathway inhibitors were used, DCs were pretreated 254 with 50 μM MG-132 (NF-κB inhibitor) (Selleckchem, US) for 1 h, 40 μM ST2825 255 (MyD88 inhibitor) (MCE, US) for 3 h, 150 μM C29 (TLR2 inhibitor) (MCE, US) for 256 1 h, 8 μM CUCPT22 (TLR2 inhibitor) (MCE, US) for 2 h or 1 μM Tak-242 (TLR4 257 inhibitor) (MCE, US) for 30 min. Then, the samples were incubated with 5 μg/ml 258 DP7-C for 2 h and subjected to Western Blot analysis. .. 259 260 T cell proliferation assay 261 To verify the effect of DCs loaded with DP7-C/OVA peptides on T cell proliferation, 262 splenocytes from OT-I and OT-II mice were separated using a lymphocyte isolation 263 solution (Dakewe Biotech Co., Ltd.) and stained with a CFSE kit (Beyotime 264 Biotechnology) according to the manufacturer’s protocol[23].



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