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cspcgt13  (ATCC)


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    Structured Review

    ATCC cspcgt13
    Cspcgt13, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cspcgt13/Paenibacillus+macerans/pmc08176775__supplementary_information_cwaa109-8-6-18
    Average 94 stars, based on 16 article reviews
    cspcgt13 - by Bioz Stars, 2026-10
    94/100 stars

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    Sequencing:

    Article Title: Engineering CGTase to improve synthesis of alkyl glycosides
    Article Snippet: .. Supplementary Figure 1 197 Carboxydocella sp. CspCGT13(AIL25792.1) Bacillus circulans 251(P43379) Bacillus sp.8SB (P30920) Paenibacillus macerans (P31835) Thermoanaerobacter sp. ATCC 53627(CAJ84380.1) Thermoanaerobacterium thermosulfurigenes EM1(P26827) Paenibacillus macerans IFO 3490(P04830) Bacillus clarkia (B9A1J6) QSDASYAENGKLYDNGVLLASYSNDPNGLFHHNGGTDFSTIEDGIYKNLFDLADLNHQNN 207 SDQPSFAENGRLYDNGTLLGGYTNDTQNLFHHNGGTDFSTTENGIYKNLYDLADLNHNNS 232 ETDTSFAENGRLYDNGTLVGGYTNDTNGYFHHNGGSDFSSLENGIYKNLYDLADFNHNNA 239 STDPSFAENGALYNNGTLLGKYSNDTAGLFHHNGGTDFSTTESGIYKNLYDLADINQNNN 232 ETDPTYGENGRLYDNGVLLGGYTNDTNGYFHHYGGTNFSSYEDGIYRNLFDLADLDQQNS 206 ETDPTYAENGRLYDNGTLLGGYTNDTNGYFHHYGGTDFSSYEDGIYRNLFDLADLNQQNS 233 RDNPGFAENGGMYDNGSLLGAYSNDTAGLFHHNGGTDFSTIEDGIYKNLYDLADINHNNN 232 ------IEDGALYDNGTLLGHYSTDANNYFYNYGGSDFSDYENSIYRNLYDLASLNQQHS 224 263 266 Carboxydocella sp. CspCGT13(AIL25792.1) Bacillus circulans 251(P43379) Bacillus sp.8SB (P30920) Paenibacillus macerans (P31835) Thermoanaerobacter sp. ATCC 53627(CAJ84380.1) Thermoanaerobacterium thermosulfurigenes EM1(P26827) Paenibacillus macerans IFO 3490(P04830) Bacillus clarkia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upplementary Figure 2 0 2 4 6 8 10 12 14 16 C1 2G 1 C1 2G 2 C1 2G 3 C1 2G 4 C1 2G 5 C1 2G 6 C1 2G 7 C1 2G 8 C1 2G 9 C1 2G 10 C1 2G 11 C1 2G 12 C1 2G 13 C1 2G 14 C1 2G 15 C1 2G 16 C1 2G 17 R el at iv e pe ak a re a (% ) Products formation during elongation of dodecyl maltoside CspCGT13 G263A/E266A F197Y/G263A F197Y/G263A/E266A Supplementary Table I. Homology modelling parameters for CspCGT13 Parameter Description Modeling speed Slow PSI-BLAST iterations in template search 3 Maximum allowed (PSI-)BLAST E-value to consider template (EValue Max) 0.5 Maximum number of templates to be used 5 Maximum number of templates with same sequence 1 Maximum oligomerization state 4 Maximum number of alignment variations per template 5 Maximum number of conformations tried per loop 50 Maximum number of residues added to the termini 10 Supplementary Table II: Validation of the CspCGT13 model Check type Quality Z-score Comment Dihedrals 1.030 Optimal Packing 1D 0.179 Optimal Packing 3D -1.733 Satisfactory Overall -0.587 Good ..

    Biomarker Discovery:

    Article Title: Engineering CGTase to improve synthesis of alkyl glycosides
    Article Snippet: .. Supplementary Figure 1 197 Carboxydocella sp. CspCGT13(AIL25792.1) Bacillus circulans 251(P43379) Bacillus sp.8SB (P30920) Paenibacillus macerans (P31835) Thermoanaerobacter sp. ATCC 53627(CAJ84380.1) Thermoanaerobacterium thermosulfurigenes EM1(P26827) Paenibacillus macerans IFO 3490(P04830) Bacillus clarkia (B9A1J6) QSDASYAENGKLYDNGVLLASYSNDPNGLFHHNGGTDFSTIEDGIYKNLFDLADLNHQNN 207 SDQPSFAENGRLYDNGTLLGGYTNDTQNLFHHNGGTDFSTTENGIYKNLYDLADLNHNNS 232 ETDTSFAENGRLYDNGTLVGGYTNDTNGYFHHNGGSDFSSLENGIYKNLYDLADFNHNNA 239 STDPSFAENGALYNNGTLLGKYSNDTAGLFHHNGGTDFSTTESGIYKNLYDLADINQNNN 232 ETDPTYGENGRLYDNGVLLGGYTNDTNGYFHHYGGTNFSSYEDGIYRNLFDLADLDQQNS 206 ETDPTYAENGRLYDNGTLLGGYTNDTNGYFHHYGGTDFSSYEDGIYRNLFDLADLNQQNS 233 RDNPGFAENGGMYDNGSLLGAYSNDTAGLFHHNGGTDFSTIEDGIYKNLYDLADINHNNN 232 ------IEDGALYDNGTLLGHYSTDANNYFYNYGGSDFSDYENSIYRNLYDLASLNQQHS 224 263 266 Carboxydocella sp. CspCGT13(AIL25792.1) Bacillus circulans 251(P43379) Bacillus sp.8SB (P30920) Paenibacillus macerans (P31835) Thermoanaerobacter sp. ATCC 53627(CAJ84380.1) Thermoanaerobacterium thermosulfurigenes EM1(P26827) Paenibacillus macerans IFO 3490(P04830) Bacillus clarkia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upplementary Figure 2 0 2 4 6 8 10 12 14 16 C1 2G 1 C1 2G 2 C1 2G 3 C1 2G 4 C1 2G 5 C1 2G 6 C1 2G 7 C1 2G 8 C1 2G 9 C1 2G 10 C1 2G 11 C1 2G 12 C1 2G 13 C1 2G 14 C1 2G 15 C1 2G 16 C1 2G 17 R el at iv e pe ak a re a (% ) Products formation during elongation of dodecyl maltoside CspCGT13 G263A/E266A F197Y/G263A F197Y/G263A/E266A Supplementary Table I. Homology modelling parameters for CspCGT13 Parameter Description Modeling speed Slow PSI-BLAST iterations in template search 3 Maximum allowed (PSI-)BLAST E-value to consider template (EValue Max) 0.5 Maximum number of templates to be used 5 Maximum number of templates with same sequence 1 Maximum oligomerization state 4 Maximum number of alignment variations per template 5 Maximum number of conformations tried per loop 50 Maximum number of residues added to the termini 10 Supplementary Table II: Validation of the CspCGT13 model Check type Quality Z-score Comment Dihedrals 1.030 Optimal Packing 1D 0.179 Optimal Packing 3D -1.733 Satisfactory Overall -0.587 Good ..



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