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crispr cas9 knockout cell lines  (Addgene inc)


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    Structured Review

    Addgene inc crispr cas9 knockout cell lines
    Crispr Cas9 Knockout Cell Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr/lentiCRISPR+v2+(Plasmid+%2352961)/pmc13693651-166-3-10
    Average 96 stars, based on 6234 article reviews
    crispr cas9 knockout cell lines - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Repurposing MDM2 inhibitor RG7388 for TP53-mutant NSCLC: a p53-independent pyroptotic mechanism via ROS/p-p38/NOXA/caspase-3/GSDME axis.
    Article Snippet: Generation of stable knockout cell lines using CRISPR–Cas 9 gene editing Lenti-CRISPRv2 (#52961), pSPAX2 (#12260) and pMD2.G (#12259) were purchased from Addgene, USA.

    Article Title: PARP inhibitor resistance in IDH1-mutant cancers due to loss of end protection factors, 53BP1 and REV7
    Article Snippet: HT1080 cells were engineered to stably express CRISPR–Cas9 using the lenti-Cas9 Blast plasmid (Addgene plasmid #52962).

    Article Title: VDAC2 loss elicits tumour destruction and inflammation for cancer therapy.
    Article Snippet: To generate tumour cells deficient for the indicated genes using CRISPR–Cas9, retrovirus was produced by co-transfecting the indicated LMA or pSIR-DsRed (BbsI) vector(s) with pCL-Eco (12371, Addgene) and VSV.G (14888, Addgene) packing plasmids into Plat-E cells.

    Article Title: Vnd and En are expressed in orthogonal stripes and act in a brief competence window to combinatorially specify NB7-1 and its early lineage.
    Article Snippet: Understanding how neuronal diversity is generated is a major goal of neuroscience.. Here we characterize the first step in generating neuronal diversity in the Drosophila embryo: spatial transcription factors (STFs) expressed in orthogonal rows and columns of neural progenitors.. These factors give spatial identity to neural progenitors (neuroblasts, NBs), and are highly conserved in mammals.

    Article Title: VDAC2 loss elicits tumour destruction and inflammation for cancer therapy
    Article Snippet: To generate tumour cells deficient for the indicated genes using CRISPR–Cas9, retrovirus was produced by co-transfecting the indicated LMA or pSIR-DsRed (BbsI) vector(s) with pCL-Eco (12371, Addgene) and VSV.G (14888, Addgene) packing plasmids into Plat-E cells.

    Article Title: Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain.
    Article Snippet: For gene editing via CRISPR–Cas9, we used the PX330 backbone (a gift from F. Zhang, Addgene plasmid 42230).

    Article Title: PARP inhibitor resistance in IDH1-mutant cancers due to loss of end protection factors, 53BP1 and REV7
    Article Snippet: REV7 sgRNA (5′-GAGGTCTTGTCGTGTGAGCG-3′) was expressed in CRISPR–Cas9-expressing HT1080 line using the lentiGuide-Puro vector (Addgene plasmid #52963).

    Generated:

    Article Title: Membrane receptors cluster phosphatidylserine to activate LC3-associated phagocytosis
    Article Snippet: .. RAW264.7- TLR2 -KO cells were generated by CRISPR–Cas9 using the LentiCRISPR-v2 system (a gift from B. Stringer; Addgene, 98290) targeting an early PAM site in the Tlr2 coding exon. .. After lentiviral transduction, the cells were selected by multiple rounds of cell sorting using a validated monoclonal antibody (clone T2.5; BioLegend, 121810) until a homogenous polyclonal negative population was obtained.

    CRISPR:

    Article Title: Membrane receptors cluster phosphatidylserine to activate LC3-associated phagocytosis
    Article Snippet: .. RAW264.7- TLR2 -KO cells were generated by CRISPR–Cas9 using the LentiCRISPR-v2 system (a gift from B. Stringer; Addgene, 98290) targeting an early PAM site in the Tlr2 coding exon. .. After lentiviral transduction, the cells were selected by multiple rounds of cell sorting using a validated monoclonal antibody (clone T2.5; BioLegend, 121810) until a homogenous polyclonal negative population was obtained.



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