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Structured Review

Proteintech craf
Craf, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/craf/RAF1+Antibody/pm41786885-180-19-38
Average 95 stars, based on 75 article reviews
craf - by Bioz Stars, 2026-09
95/100 stars

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Related Articles

Blocking Assay:

Article Title: Actin-modulated nuclear shape controls adaptive reprogramming in cancer-associated fibroblasts
Article Snippet: .. Afterwards, the cells were blocked with Duolink blocking solution for one hour at 37°C and then incubated overnight at 4°C with the following primary antibodies: BRAF (Proteintech, 20899-1-AP), CRAF (Proteintech, clone 1D6A1), FLAG (Proteintech, 20543-1-AP), or MYC (Proteintech, clone 1A5A2). ..

Article Title: Actin-modulated nuclear shape controls adaptive reprogramming in cancer-associated fibroblasts
Article Snippet: .. The membranes were blocked with 5% fat-free milk or 5% BSA in TBST for one hour at room temperature and then incubated with the following primary antibodies: ARAF (Cell Signaling Technology, 4432, 1:1000), BRAF (Proteintech, 20899-1-AP, 1:1000), CRAF (Proteintech, clone 1D6A1, 1:1000), CRAF (Proteintech, 26863-1-AP, 1:1000), phospho-BRAF (Cell Signaling Technology, 2696, 1:1000), phospho-CRAF (Cell Signaling Technology, 9127, 1:1000), Flag (Proteintech, 20543-1-AP, 1:1000), MYC (Proteintech, clone 1A5A2, 1:1000), MYC (Cell Signaling Technology, clone 71D10, 1:1000), KRAS (Proteintech, 12063-1-AP, 1:1000), HRAS (Proteintech, 18295-1-AP, 1:1000), NRAS (Proteintech, 10724-1-AP, 1:1000), ERK1/2 (Cell Signaling Technology, clone 3A7, 1:1000), and phospho-ERK (Cell Signaling Technology, clone D13.14) in blocking buffer. .. After being washed three times with TBST, the membranes were then incubated with IRDye 800CW donkey anti-rabbit IgG secondary antibody (LI-COR, 926–32213, 1:2000) or IRDye 680RD donkey anti-mouse IgG secondary antibody (LI-COR, 926–68072, 1:2000) at a 1:2000 dilution in blocking buffer and washed three times with TBST.

Incubation:

Article Title: Actin-modulated nuclear shape controls adaptive reprogramming in cancer-associated fibroblasts
Article Snippet: .. Afterwards, the cells were blocked with Duolink blocking solution for one hour at 37°C and then incubated overnight at 4°C with the following primary antibodies: BRAF (Proteintech, 20899-1-AP), CRAF (Proteintech, clone 1D6A1), FLAG (Proteintech, 20543-1-AP), or MYC (Proteintech, clone 1A5A2). ..

Article Title: Actin-modulated nuclear shape controls adaptive reprogramming in cancer-associated fibroblasts
Article Snippet: .. The membranes were blocked with 5% fat-free milk or 5% BSA in TBST for one hour at room temperature and then incubated with the following primary antibodies: ARAF (Cell Signaling Technology, 4432, 1:1000), BRAF (Proteintech, 20899-1-AP, 1:1000), CRAF (Proteintech, clone 1D6A1, 1:1000), CRAF (Proteintech, 26863-1-AP, 1:1000), phospho-BRAF (Cell Signaling Technology, 2696, 1:1000), phospho-CRAF (Cell Signaling Technology, 9127, 1:1000), Flag (Proteintech, 20543-1-AP, 1:1000), MYC (Proteintech, clone 1A5A2, 1:1000), MYC (Cell Signaling Technology, clone 71D10, 1:1000), KRAS (Proteintech, 12063-1-AP, 1:1000), HRAS (Proteintech, 18295-1-AP, 1:1000), NRAS (Proteintech, 10724-1-AP, 1:1000), ERK1/2 (Cell Signaling Technology, clone 3A7, 1:1000), and phospho-ERK (Cell Signaling Technology, clone D13.14) in blocking buffer. .. After being washed three times with TBST, the membranes were then incubated with IRDye 800CW donkey anti-rabbit IgG secondary antibody (LI-COR, 926–32213, 1:2000) or IRDye 680RD donkey anti-mouse IgG secondary antibody (LI-COR, 926–68072, 1:2000) at a 1:2000 dilution in blocking buffer and washed three times with TBST.



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