Journal: BMC Complementary Medicine and Therapies
Article Title: Inhibition of the PI3K signaling pathway in cancer cells by Agrimonia eupatoria L. ethanolic extract: identification of tricoumaroyl spermidine as a potential PI3K inhibitor
doi: 10.1186/s12906-025-05231-z
Figure Lengend Snippet: Growth inhibition of HT-29 (panel A ), A549 (panel B ), MCF-7 (panel C ), HeLa (panel D and CCD 841 CON (panel E ) cells treated with AE extracts for different exposure times assessed using MTT assay. The grey broken lines in the graphs mark the level of IC10 and IC 50 values corresponding to 90% and 50% cell growth, respectively, compared to control (100%). The heatmap of IC 50 values on AE extract on different cell lines (panel G ). The genotoxic effects of AE on HT-29 cells expressed as % DNA in the comet tail (panel F ). Results represent means ± SD from three independent experiments carried out in triplicates. The p-value for 0.5 mg DW/mL concentration was 0.0060
Article Snippet: MCF-7 (ATCC HTB‐22) (human breast cancer), HT-29 (ATCC HTB‐38) (Human colon adenocarcinoma), A549 (ATCC CCL-185) (human lung adenocarcinoma) and HeLa (ATCC CCL-2) (human cervical carcinoma) CCD 841 CoN (ATCC CRL-1790) (non-cancerous human colon fibroblast cells) cell cultures were obtained from ATCC.
Techniques: Inhibition, MTT Assay, Control, Concentration Assay