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cnp
Cnp, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cnp/C-type+natriuretic+peptide+(1-22)+(human%2C+rat%2C+swine)/bio_rxiv__64898__2026__03__13__711248-170-23-24
Average 94 stars, based on 8 article reviews
Cnp, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cnp/C-type+natriuretic+peptide+(1-22)+(human%2C+rat%2C+swine)/bio_rxiv__64898__2026__03__13__711248-170-23-24
Average 94 stars, based on 8 article reviews
cnp - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Neprilysin inhibition reduces microtubule detyrosination in cardiomyocytes through a cGMP-PRKG1-VASH1 axis"
Article Title: Neprilysin inhibition reduces microtubule detyrosination in cardiomyocytes through a cGMP-PRKG1-VASH1 axis
Journal: bioRxiv
doi: 10.64898/2026.03.13.711248
Figure Legend Snippet: (A) Protocol: VASH1-KO hiPSC-CMs were kept in maturation medium for 2 weeks and then transduced for 6 days with lentivirus expressing YFP-tagged VASH1-WT, VASH1-7E or VASH1-7A each in combination with SVBP. (B) Representative Western blot of crude hiPSC-CM protein lysates stained for YFP-VASH1 and GAPDH (loading control) and quantification of VASH1/GAPDH in the 3 conditions (N/d=6/1). (C) Representative Western blot of crude hiPSC-CM protein lysates stained for YFP-VASH1, dTyr-tub, Tyr-tub and GAPDH (loading control) and quantification of dTyr-tub/GAPDH and Tyr-tub/GAPDH in non-transduced VASH1-KO hiPSC-CMs or transduced with YFP-VASH1-WT in combination with SVBP (N/d=6/1). (D) Representative Western blot of crude hiPSC-CM protein lysates stained for YFP-VASH1, dTyr-tub, Tyr-tub and GAPDH (loading control) and quantification of dTyr-tub/GAPDH and Tyr-tub/GAPDH in VASH1-KO hiPSC-CMs transduced with YFP-VASH1-7E or YFP-VASH1-7A in combination with SVBP (N/d=6/1). (E) Proposed model of regulation of VASH1 activity downstream of the action of sac. Sac inhibits neprilysin that would otherwise degrade the natriuretic peptides ANP, BNP and CNP. Through this inhibition, these natriuretic peptides can bind their respective receptors, which leads to the intracellular production of cGMP. Increased amounts of intracellular cGMP would then activate PRKG1A and thereby phosphorylate different targets, including VASH1. Eventually, phosphorylation of VASH1 reduces the binding of VASH1 to microtubules and tubulin detyrosination. Data are expressed as mean±SEM. Statistical significance was assessed with the one-way ANOVA and Tukey’s multiple comparisons tests (panel B) or unpaired Student’s t -test (panels C and D). Abbreviations: ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; cGMP, guanosine 3′,5′-cyclic monophosphate; CNP, C-type natriuretic peptide; dTyr-tub, detyrosinated tubulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GTP, guanosine triphosphate; hiPSC-CMs, human induced pluripotent stem cell-derived cardiomyocytes; KO, knock-out; MW, molecular weight marker; N/d, number of wells/differentiations; NPRA, natriuretic peptide receptor 1; NPRB, natriuretic peptide receptor 2; P, phosphorylated; pGC, particulate guanylate cyclase; PRKG1A, cGMP-dependent protein kinase G1 alpha; Tyr-tub, tyrosinated tubulin; VASH1, vasohibin 1; VASH1-7A, non-phosphorylatable VASH1 with 7 alanine residues; VASH1-7E, VASH1 phosphomimic with 7 glutamate residues; WT, wild-type.
Techniques Used: Expressing, Western Blot, Staining, Control, Transduction, Activity Assay, Inhibition, Phospho-proteomics, Binding Assay, Derivative Assay, Knock-Out, Molecular Weight, Marker
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