Concentration Assay:Article Title: The impact of fatty acid synthase on HSV-1 infection dynamics.
Article Snippet: .. CMS121 (HY-135981, MedChemExpress), C75 (HY-12364, MedChemExpress), SSO (11211, Cayman Chemical), palmitate (P0500; Sigma-Aldrich), and BODIPY FL C16 (D3821; Invitrogen) were used at a concentration of 5 μM, 5 μM, 200 μM, 200 μM, and 2 μM, respectively. .. PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013068 May 6, 2025 15 / 21 pLKO.1 puro-humanU6-shRNA FASN was a gift from Elizabeth Stoll (Addgene plasmid #82327; http://n2t.net/ addgene:82327; RRID: Addgene_82327). pLKO.1 Puro shRNA Scramble was a gift from Antonia Follenzi (University of Piemonte Orientale, Novara, Italy). pAcUW51-CgE was a gift from Pamela Bjorkman (Addgene plasmid #13762; http:// n2t.net/addgene:13762; RRID: Addgene_13762) [33].
Article Title: The impact of fatty acid synthase on HSV-1 infection dynamics
Article Snippet: .. CMS121 (HY-135981, MedChemExpress), C75 (HY-12364, MedChemExpress), SSO (11211, Cayman Chemical), palmitate (P0500; Sigma-Aldrich), and BODIPY FL C16 (D3821; Invitrogen) were used at a concentration of 5 μM, 5 μM, 200 μM, 200 μM, and 2 μM, respectively. pLKO.1 puro-humanU6-shRNA FASN was a gift from Elizabeth Stoll (Addgene plasmid #82327; http://n2t.net/addgene:82327 ; RRID: Addgene_82327). pLKO.1 Puro shRNA Scramble was a gift from Antonia Follenzi (University of Piemonte Orientale, Novara, Italy). pAcUW51-CgE was a gift from Pamela Bjorkman (Addgene plasmid #13762; http://n2t.net/addgene:13762 ; RRID: Addgene_13762) [ ]. .. Recombinant lentiviruses were packaged in HEK 293T cells by cotransfection of the 3 rd Generation Packaging System Mix (kindly provided by Antonia Follenzi, University of Piemonte Orientale, Novara, Italy) with the above-mentioned vectors to produce viral particles using Lipofectamine 2000 (Thermo Fisher Scientific).
Plasmid Preparation:Article Title: The impact of fatty acid synthase on HSV-1 infection dynamics
Article Snippet: .. CMS121 (HY-135981, MedChemExpress), C75 (HY-12364, MedChemExpress), SSO (11211, Cayman Chemical), palmitate (P0500; Sigma-Aldrich), and BODIPY FL C16 (D3821; Invitrogen) were used at a concentration of 5 μM, 5 μM, 200 μM, 200 μM, and 2 μM, respectively. pLKO.1 puro-humanU6-shRNA FASN was a gift from Elizabeth Stoll (Addgene plasmid #82327; http://n2t.net/addgene:82327 ; RRID: Addgene_82327). pLKO.1 Puro shRNA Scramble was a gift from Antonia Follenzi (University of Piemonte Orientale, Novara, Italy). pAcUW51-CgE was a gift from Pamela Bjorkman (Addgene plasmid #13762; http://n2t.net/addgene:13762 ; RRID: Addgene_13762) [ ]. .. Recombinant lentiviruses were packaged in HEK 293T cells by cotransfection of the 3 rd Generation Packaging System Mix (kindly provided by Antonia Follenzi, University of Piemonte Orientale, Novara, Italy) with the above-mentioned vectors to produce viral particles using Lipofectamine 2000 (Thermo Fisher Scientific).
shRNA:Article Title: The impact of fatty acid synthase on HSV-1 infection dynamics
Article Snippet: .. CMS121 (HY-135981, MedChemExpress), C75 (HY-12364, MedChemExpress), SSO (11211, Cayman Chemical), palmitate (P0500; Sigma-Aldrich), and BODIPY FL C16 (D3821; Invitrogen) were used at a concentration of 5 μM, 5 μM, 200 μM, 200 μM, and 2 μM, respectively. pLKO.1 puro-humanU6-shRNA FASN was a gift from Elizabeth Stoll (Addgene plasmid #82327; http://n2t.net/addgene:82327 ; RRID: Addgene_82327). pLKO.1 Puro shRNA Scramble was a gift from Antonia Follenzi (University of Piemonte Orientale, Novara, Italy). pAcUW51-CgE was a gift from Pamela Bjorkman (Addgene plasmid #13762; http://n2t.net/addgene:13762 ; RRID: Addgene_13762) [ ]. .. Recombinant lentiviruses were packaged in HEK 293T cells by cotransfection of the 3 rd Generation Packaging System Mix (kindly provided by Antonia Follenzi, University of Piemonte Orientale, Novara, Italy) with the above-mentioned vectors to produce viral particles using Lipofectamine 2000 (Thermo Fisher Scientific).
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