Journal: Aging Cell
Article Title: Graded Calorie Restriction Causes Graded Slowing of Epigenetic Ageing in Mice
doi: 10.1111/acel.70342
Figure Lengend Snippet: Slowing of Epigenetic Ageing. (A) values for 12‐h ab‐lib (12AL) feeding and 40% calorie restriction (40CR) at 24 months after 19 months calorie restriction (CR) relative to 12AL feeding at 8 months after 3 months CR (filled triangle 24‐month 12AL, open triangle 24‐month 40CR, filled circle 8‐month 12AL, error are SEM). (B) Normalised DNA difference of 24‐month 40CR samples versus 8‐ and 24‐month 12AL (filled black circle DNA methylation decreases from 8‐month to 24 month ad‐lib feeding, open black circle DNA methylation increases from 8 month to 24 month ad‐lib feeding). One data point of 2.31 (decreasing) for gene Fam84b is omitted from the graph. The average position of all 24‐month mice 40CR data is equivalent to mice aged about 12 months. The gene/intergenic regions are: 1 Dnajb2, 2 Brinp2, 3 Clvs2, 4 Adamtsl5, 5 Nfic, 6 Tlx3, 7 Sox30, 8 intergenic1, 9 Stard3, 10 Fzd2, 11 intergenic2, 12 Grk6, 13 Scara5, 14 Fam84b, 15 Lynx1, 16 Lncppara, 17 Gm26518, 18 Ddr1, 19 Hdgfl2, 20 Cd5, 21 Slc16a12, 22 Dido1, 23 Proser1, 24 Nbea, 25 intergenic3, 26 intergenic4, 27 intergenic5, 28 Gm10463, 29 Cldn4, 30 intergenic5, 31 Wasf3, 32 intergenic6, 33 Qprt, 34 Nlrp6, 35 intergenic7, 36 Cdh8, 37 Map10, 38 Rbpms2, 39 Zic1.
Article Snippet: Results here employ the epigenetic clock DNAge (Zymo Research) developed for mice (Coninx et al. ).
Techniques: DNA Methylation Assay