Review




Structured Review

Proteintech birc3
Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and <t>BIRC3</t> ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group
Birc3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ciap2/cIAP2+Antibody/pmc12495749-72-15-16
Average 93 stars, based on 31 article reviews
birc3 - by Bioz Stars, 2026-09
93/100 stars

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1) Product Images from "Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35"

Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

Journal: BMC Cancer

doi: 10.1186/s12885-025-14925-z

Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group
Figure Legend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Techniques Used: Transfection

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Incubation:

Article Title: A prognostic mathematical model based on tumor microenvironment-related genes expression for breast cancer patients
Article Snippet: .. After washing in phosphate buffered saline (3 min × 3), sections were blocked in goat serum at room temperature for 30 min and incubated with primary antibodies (PSMB8, (1:200), Proteintech Group, IL, USA; cIAP2, (1:200), Proteintech Group, IL, USA) overnight at 4° C. The next day, sections were incubated with secondary antibodies (Maxin Biotechnologies, China) and treated with diaminobenzidine hydrochloride to visualize immunoreactivity. ..

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Article Title: Arsenic-induced hepatotoxicity: Mechanistic insights into TNFAIP3-mediated regulation of the NF-κB p65/IAPs Signaling Pathway.
Article Snippet: Denatured protein samples were separated by electrophoresis using 10–15 % sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) and subsequently transferred to a 0.22 μm polyvinylidene fluoride (PVDF) membrane (Millipore Corporation, USA). .. The membrane was blocked with 5 % skimmed milk or bovine serum albumin (BSA) at room temperature for 1.5 h, followed by incubation with primary antibodies: β-actin (1:10000, Abclonal); cIAP1, cIAP2 and XIAP (1:1000, Abcam); Survivin and TNF-α (1:500, CST); NF-κB p65 and p-NF-κB p65 (1:1000, CST); TNFAIP3 (1:1000, Affinity); Caspase 3 (1:500, Abcam); Histone-H3 (1:1000, Proteintech) at 4 °C overnight. .. The following day, the membranes were washed four times with TBST and incubated with a horseradish peroxidase (HRP)-conjugated immunoglobulin G (IgG) secondary antibody (1:5000, Immunoway) at room temperature for 1 h. After repeating the washing process, the membranes were treated with enhanced chemiluminescence (ECL, Biosharp).

Article Title: Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.
Article Snippet: .. After that, the PVDF membrane was incubated overnight in primary antibody (ANXA2 (Dilution ratio is 1:1000), NF-κB p105/p50 (1:1000), cIAP1 (1:1000) and cIAP2 (1:1000) specific antibody were purchased from Proteintech (Wuhan, China); actin specific antibody (1:10000) was purchased from Sino biological (Beijing, China)). .. The PVDF membrane was washed 3 times (10⬰min each time) with PBST (0.2 % Tween) on a shaker and then incubated in secondary antibody (1:10000) (Proteintech, Wuhan, China) at 37⬰◦C for 1⬰h.

Article Title: Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells
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Image Search Results


Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Journal: BMC Cancer

Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

doi: 10.1186/s12885-025-14925-z

Figure Lengend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Article Snippet: The primary antibodies against USP35 (proteintech, Wuhan, China, 24559-1-AP), PKM2 (proteintech, 15822-1-AP), FPN (proteintech, 26601-1-AP), BIRC3 (proteintech, 24304-1-AP), β-actin (proteintech, 20536-1-AP) were incubated overnight at 4°C.

Techniques: Transfection