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chga  (Proteintech)


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    Structured Review

    Proteintech chga
    Chga, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chga/PROX1+Antibody/pm41881959-314-15-16
    Average 94 stars, based on 94 article reviews
    chga - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: A novel scheme for non-invasive drug delivery with a magnetically controlled drug delivering capsule endoscope.
    Article Snippet: There is a lack of effective means for precise drug delivery of gastrointestinal diseases.. Herein we report a novel magnetically controlled drug delivering capsule endoscope (MDCE) to achieve precision drug delivery for gastrointestinal diseases.. MDCE integrates a drug delivery system into conventional capsule endoscope.

    Article Title: Comparison of the Construction Methods of Parathyroid Organoids and Functional Influence of Calcium on Organoids.
    Article Snippet: .. Briefly, paraffinembedded organoid sections were initially processed following the H&E staining protocol up to the hydration step, where the sections were immersed in double- steaming water for 5 min. Antigen retrieval was performed using citrate buffer (10 mM sodium citrate with 0.05% Tween- 20, pH 6.0), which was preheated by microwave until boiling, and sections were incubated in the buffer at 95 °C for 30 min. After cooling and PBS rinsing (2 min), sections were blocked with 3% BSA at room temperature for 1 h. Primary antibodies used included PTH (Proteintech, #10817- 1- AP), GCM2 (Proteintech, #21088- 1- AP), CHGA (Proteintech, #10529- 1- AP), and CaSR (Proteintech, #19125- 1- AP), applied overnight at 4 °C in a humidified chamber according to the experimental group. ..

    Immunohistochemistry:

    Article Title: New insights into markers for distinguishing neuroendocrine prostate cancer: evidence from single-cell analysis
    Article Snippet: Prostate tissues from patients (including 2 cases of primary PCa and 2 cases of NEPC) who underwent radical prostatectomy in the Department of Urology at the First Affiliated Hospital of Anhui Medical University were selected for this analysis. .. We conducted IHC staining to evaluate the expression of NSE (NSE Polyclonal antibody, Cat# 10149-1-AP, RRID: AB_2099180, Proteintech, USA), CHGA (Chromogranin A Polyclonal antibody, Cat# 23342-1-AP, RRID: AB_2879259, Proteintech, USA), ASCL1(ASCL1 Polyclonal antibody, Cat# 23751-1-AP, RRID: AB_2935459, Proteintech, USA) and WDFY4 (WDFY4 Polyclonal antibody, Cat# 17558-1-AP, RRID: AB_2288447, Proteintech, USA). ..

    Staining:

    Article Title: New insights into markers for distinguishing neuroendocrine prostate cancer: evidence from single-cell analysis
    Article Snippet: Prostate tissues from patients (including 2 cases of primary PCa and 2 cases of NEPC) who underwent radical prostatectomy in the Department of Urology at the First Affiliated Hospital of Anhui Medical University were selected for this analysis. .. We conducted IHC staining to evaluate the expression of NSE (NSE Polyclonal antibody, Cat# 10149-1-AP, RRID: AB_2099180, Proteintech, USA), CHGA (Chromogranin A Polyclonal antibody, Cat# 23342-1-AP, RRID: AB_2879259, Proteintech, USA), ASCL1(ASCL1 Polyclonal antibody, Cat# 23751-1-AP, RRID: AB_2935459, Proteintech, USA) and WDFY4 (WDFY4 Polyclonal antibody, Cat# 17558-1-AP, RRID: AB_2288447, Proteintech, USA). ..

    Article Title: Comparison of the Construction Methods of Parathyroid Organoids and Functional Influence of Calcium on Organoids.
    Article Snippet: .. Briefly, paraffinembedded organoid sections were initially processed following the H&E staining protocol up to the hydration step, where the sections were immersed in double- steaming water for 5 min. Antigen retrieval was performed using citrate buffer (10 mM sodium citrate with 0.05% Tween- 20, pH 6.0), which was preheated by microwave until boiling, and sections were incubated in the buffer at 95 °C for 30 min. After cooling and PBS rinsing (2 min), sections were blocked with 3% BSA at room temperature for 1 h. Primary antibodies used included PTH (Proteintech, #10817- 1- AP), GCM2 (Proteintech, #21088- 1- AP), CHGA (Proteintech, #10529- 1- AP), and CaSR (Proteintech, #19125- 1- AP), applied overnight at 4 °C in a humidified chamber according to the experimental group. ..

    Expressing:

    Article Title: New insights into markers for distinguishing neuroendocrine prostate cancer: evidence from single-cell analysis
    Article Snippet: Prostate tissues from patients (including 2 cases of primary PCa and 2 cases of NEPC) who underwent radical prostatectomy in the Department of Urology at the First Affiliated Hospital of Anhui Medical University were selected for this analysis. .. We conducted IHC staining to evaluate the expression of NSE (NSE Polyclonal antibody, Cat# 10149-1-AP, RRID: AB_2099180, Proteintech, USA), CHGA (Chromogranin A Polyclonal antibody, Cat# 23342-1-AP, RRID: AB_2879259, Proteintech, USA), ASCL1(ASCL1 Polyclonal antibody, Cat# 23751-1-AP, RRID: AB_2935459, Proteintech, USA) and WDFY4 (WDFY4 Polyclonal antibody, Cat# 17558-1-AP, RRID: AB_2288447, Proteintech, USA). ..



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    a Scheme for the generation of an inducible β-cell-specific TBL1X and TBL1XR1 knockout. b Random fed blood glucose levels over time in iTBL/RβKO ( n = 9) and control ( n = 9) mice on HFD. c Total pancreas insulin content normalized to protein levels in iTBL/RβKO and control mice 4 and 11 weeks after tamoxifen administration. n = 3 for 4w control and iTBL/RβKO mice, n = 6 for 11w control mice, n = 4 for 11w iTBL/RβKO mice. d , e Blood glucose ( d ) and plasma insulin ( e ) levels during an oral glucose tolerance test after 22 weeks of HFD. Corresponding area under the curve in iTBL/RβKO ( n = 8) and control ( n = 9) mice ( d , right). f , g Relative mRNA expression determined by qPCR in pancreatic islets of iTBL/RβKO and control mice of Tbl1x and Tbl1xr1 ( f ) and islet genes ( g ). Control mice Tbl1x , Tbl1xr1 , Ins1 , Ins2 , Nkx6.1 , Slc2a2 , Mafa , Pdx1 , Pax6 , Ucn3 : n = 9, control mice <t>ChgA</t> , Ngn3 , Ldha , Hk1 : n = 8, iTBL/RβKO mice Tbl1x , Ngn3 , Ldha , Hk1 : n = 7, iTBL/RβKO mice Tbl1xr1 , Ins1 , Ins2 , Slc2a2 , Mafa n = 6, iTBL/RβKO mice Nkx6.1 , Pdx1 , Pax6 , Ucn3 , ChgA : n = 5. h α/β-cell mass ratio of pancreatic islets from iTBL/RβKO ( n = 4) and control mice ( n = 4) on HFD, 24 weeks after knockout induction. i Representative immunofluorescent staining of insulin + (blue, β-cells) and glucagon + (red, α-cells) cells of paraffin-embedded pancreas from iTBL/RβKO and control mice on HFD, 24 weeks after knockout induction. Each point represents one mouse. Data are represented as mean ± SEM. The following statistical tests were applied: two-sided student’s t test ( d – Area under the curve, f – h ) and 2-way ANOVA with Šidák’s multiple comparison post hoc test ( b – d – time course, e ). Source data are provided as a Source Data file.
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    chga  (Proteintech)
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    a Scheme for the generation of an inducible β-cell-specific TBL1X and TBL1XR1 knockout. b Random fed blood glucose levels over time in iTBL/RβKO ( n = 9) and control ( n = 9) mice on HFD. c Total pancreas insulin content normalized to protein levels in iTBL/RβKO and control mice 4 and 11 weeks after tamoxifen administration. n = 3 for 4w control and iTBL/RβKO mice, n = 6 for 11w control mice, n = 4 for 11w iTBL/RβKO mice. d , e Blood glucose ( d ) and plasma insulin ( e ) levels during an oral glucose tolerance test after 22 weeks of HFD. Corresponding area under the curve in iTBL/RβKO ( n = 8) and control ( n = 9) mice ( d , right). f , g Relative mRNA expression determined by qPCR in pancreatic islets of iTBL/RβKO and control mice of Tbl1x and Tbl1xr1 ( f ) and islet genes ( g ). Control mice Tbl1x , Tbl1xr1 , Ins1 , Ins2 , Nkx6.1 , Slc2a2 , Mafa , Pdx1 , Pax6 , Ucn3 : n = 9, control mice <t>ChgA</t> , Ngn3 , Ldha , Hk1 : n = 8, iTBL/RβKO mice Tbl1x , Ngn3 , Ldha , Hk1 : n = 7, iTBL/RβKO mice Tbl1xr1 , Ins1 , Ins2 , Slc2a2 , Mafa n = 6, iTBL/RβKO mice Nkx6.1 , Pdx1 , Pax6 , Ucn3 , ChgA : n = 5. h α/β-cell mass ratio of pancreatic islets from iTBL/RβKO ( n = 4) and control mice ( n = 4) on HFD, 24 weeks after knockout induction. i Representative immunofluorescent staining of insulin + (blue, β-cells) and glucagon + (red, α-cells) cells of paraffin-embedded pancreas from iTBL/RβKO and control mice on HFD, 24 weeks after knockout induction. Each point represents one mouse. Data are represented as mean ± SEM. The following statistical tests were applied: two-sided student’s t test ( d – Area under the curve, f – h ) and 2-way ANOVA with Šidák’s multiple comparison post hoc test ( b – d – time course, e ). Source data are provided as a Source Data file.
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    a Scheme for the generation of an inducible β-cell-specific TBL1X and TBL1XR1 knockout. b Random fed blood glucose levels over time in iTBL/RβKO ( n = 9) and control ( n = 9) mice on HFD. c Total pancreas insulin content normalized to protein levels in iTBL/RβKO and control mice 4 and 11 weeks after tamoxifen administration. n = 3 for 4w control and iTBL/RβKO mice, n = 6 for 11w control mice, n = 4 for 11w iTBL/RβKO mice. d , e Blood glucose ( d ) and plasma insulin ( e ) levels during an oral glucose tolerance test after 22 weeks of HFD. Corresponding area under the curve in iTBL/RβKO ( n = 8) and control ( n = 9) mice ( d , right). f , g Relative mRNA expression determined by qPCR in pancreatic islets of iTBL/RβKO and control mice of Tbl1x and Tbl1xr1 ( f ) and islet genes ( g ). Control mice Tbl1x , Tbl1xr1 , Ins1 , Ins2 , Nkx6.1 , Slc2a2 , Mafa , Pdx1 , Pax6 , Ucn3 : n = 9, control mice <t>ChgA</t> , Ngn3 , Ldha , Hk1 : n = 8, iTBL/RβKO mice Tbl1x , Ngn3 , Ldha , Hk1 : n = 7, iTBL/RβKO mice Tbl1xr1 , Ins1 , Ins2 , Slc2a2 , Mafa n = 6, iTBL/RβKO mice Nkx6.1 , Pdx1 , Pax6 , Ucn3 , ChgA : n = 5. h α/β-cell mass ratio of pancreatic islets from iTBL/RβKO ( n = 4) and control mice ( n = 4) on HFD, 24 weeks after knockout induction. i Representative immunofluorescent staining of insulin + (blue, β-cells) and glucagon + (red, α-cells) cells of paraffin-embedded pancreas from iTBL/RβKO and control mice on HFD, 24 weeks after knockout induction. Each point represents one mouse. Data are represented as mean ± SEM. The following statistical tests were applied: two-sided student’s t test ( d – Area under the curve, f – h ) and 2-way ANOVA with Šidák’s multiple comparison post hoc test ( b – d – time course, e ). Source data are provided as a Source Data file.
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    Image Search Results


    a Scheme for the generation of an inducible β-cell-specific TBL1X and TBL1XR1 knockout. b Random fed blood glucose levels over time in iTBL/RβKO ( n = 9) and control ( n = 9) mice on HFD. c Total pancreas insulin content normalized to protein levels in iTBL/RβKO and control mice 4 and 11 weeks after tamoxifen administration. n = 3 for 4w control and iTBL/RβKO mice, n = 6 for 11w control mice, n = 4 for 11w iTBL/RβKO mice. d , e Blood glucose ( d ) and plasma insulin ( e ) levels during an oral glucose tolerance test after 22 weeks of HFD. Corresponding area under the curve in iTBL/RβKO ( n = 8) and control ( n = 9) mice ( d , right). f , g Relative mRNA expression determined by qPCR in pancreatic islets of iTBL/RβKO and control mice of Tbl1x and Tbl1xr1 ( f ) and islet genes ( g ). Control mice Tbl1x , Tbl1xr1 , Ins1 , Ins2 , Nkx6.1 , Slc2a2 , Mafa , Pdx1 , Pax6 , Ucn3 : n = 9, control mice ChgA , Ngn3 , Ldha , Hk1 : n = 8, iTBL/RβKO mice Tbl1x , Ngn3 , Ldha , Hk1 : n = 7, iTBL/RβKO mice Tbl1xr1 , Ins1 , Ins2 , Slc2a2 , Mafa n = 6, iTBL/RβKO mice Nkx6.1 , Pdx1 , Pax6 , Ucn3 , ChgA : n = 5. h α/β-cell mass ratio of pancreatic islets from iTBL/RβKO ( n = 4) and control mice ( n = 4) on HFD, 24 weeks after knockout induction. i Representative immunofluorescent staining of insulin + (blue, β-cells) and glucagon + (red, α-cells) cells of paraffin-embedded pancreas from iTBL/RβKO and control mice on HFD, 24 weeks after knockout induction. Each point represents one mouse. Data are represented as mean ± SEM. The following statistical tests were applied: two-sided student’s t test ( d – Area under the curve, f – h ) and 2-way ANOVA with Šidák’s multiple comparison post hoc test ( b – d – time course, e ). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory network

    doi: 10.1038/s41467-026-72077-5

    Figure Lengend Snippet: a Scheme for the generation of an inducible β-cell-specific TBL1X and TBL1XR1 knockout. b Random fed blood glucose levels over time in iTBL/RβKO ( n = 9) and control ( n = 9) mice on HFD. c Total pancreas insulin content normalized to protein levels in iTBL/RβKO and control mice 4 and 11 weeks after tamoxifen administration. n = 3 for 4w control and iTBL/RβKO mice, n = 6 for 11w control mice, n = 4 for 11w iTBL/RβKO mice. d , e Blood glucose ( d ) and plasma insulin ( e ) levels during an oral glucose tolerance test after 22 weeks of HFD. Corresponding area under the curve in iTBL/RβKO ( n = 8) and control ( n = 9) mice ( d , right). f , g Relative mRNA expression determined by qPCR in pancreatic islets of iTBL/RβKO and control mice of Tbl1x and Tbl1xr1 ( f ) and islet genes ( g ). Control mice Tbl1x , Tbl1xr1 , Ins1 , Ins2 , Nkx6.1 , Slc2a2 , Mafa , Pdx1 , Pax6 , Ucn3 : n = 9, control mice ChgA , Ngn3 , Ldha , Hk1 : n = 8, iTBL/RβKO mice Tbl1x , Ngn3 , Ldha , Hk1 : n = 7, iTBL/RβKO mice Tbl1xr1 , Ins1 , Ins2 , Slc2a2 , Mafa n = 6, iTBL/RβKO mice Nkx6.1 , Pdx1 , Pax6 , Ucn3 , ChgA : n = 5. h α/β-cell mass ratio of pancreatic islets from iTBL/RβKO ( n = 4) and control mice ( n = 4) on HFD, 24 weeks after knockout induction. i Representative immunofluorescent staining of insulin + (blue, β-cells) and glucagon + (red, α-cells) cells of paraffin-embedded pancreas from iTBL/RβKO and control mice on HFD, 24 weeks after knockout induction. Each point represents one mouse. Data are represented as mean ± SEM. The following statistical tests were applied: two-sided student’s t test ( d – Area under the curve, f – h ) and 2-way ANOVA with Šidák’s multiple comparison post hoc test ( b – d – time course, e ). Source data are provided as a Source Data file.

    Article Snippet: The following TaqMan probes were used: Tbp - Mm01277042_m1, Tbl1x - Mm01222202_m1, Tbl1xr1 - Mm01283877_m1, Ins1 - Mm01259683_g1, Ins2 - Mm00731595_gH, Nkx6.1 - Mm00454961_m1, Slc2a2 - Mm00454961_m1, Mafa - Mm00845206_s1, Pdx1 - Mm00435565_m1, Pax6 - Mm00443081_m1, Ucn3 - Mm00453206_s1, ChgA - Mm00514341_m1, Ngn3 - Mm00437606_s1, Ldha - Mm01612132_g1, Hk1 - Mm00439344_m1, TBP - Hs00427620_m1, TBL1X - Hs00959540_m1, TBL1XR1 - Hs00226564_m1.

    Techniques: Knock-Out, Control, Clinical Proteomics, Expressing, Staining, Comparison