rabbit polyclonal anti cfim68 (Bethyl)

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Structured Review

Bethyl rabbit polyclonal anti cfim68
Rabbit Polyclonal Anti Cfim68, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfim68/CPSF68+Antibody/pmc12999487-408-42-45
Average 93 stars, based on 32 article reviews

rabbit polyclonal anti cfim68 - by Bioz Stars, 2026-10

93/100 stars

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Chromatin Immunoprecipitation:

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: ChIP-seq libraries were prepared using the ChIP-IT® Express Enzymatic Shearing (Active Motif) and ChIP-seq library preparation (Vazyme) kits. .. Primary antibodies used for ChIP included RNAPII (39497, Active Motif); hnRNPL (18354-1-AP, Proteintech); RNAPII Ser5 (61986, Active Motif); RNAPII Ser2 (61984, Active Motif); LEO1 (PAB14102, Abnova); CFIm68 (A301-358A, Bethyl); CFIm59 (A301-359A, Bethyl). .. ChIP-seq libraries for CFIm25 were prepared using the CUT & Tag Hyperactive In-Situ ChIP Library Prep Kit (Vazyme) and primary antibody (10322-1-AP, Proteintech).

Article Title: U1 AMO (antisense morpholino oligo) disrupts U1 snRNP structure to promote intronic premature cleavage and polyadenylation (PCPA)
Article Snippet: ChIP-seq libraries were prepared using the ChIP-IT ® Express Enzymatic Shearing (Active Motif) and ChIP-seq library preparation (Vazyme) kits. .. Primary antibodies used for ChIP included RNAPII Ser5P (61,986, Active Motif); RNAPII Ser2P (61,984, Active Motif); PCF11(A303-706A, Bethyl); CPSF160(A301-580A, Bethyl); WDR33(A301-152A, Bethyl); CstF77(A301-094A, Bethyl); CPSF100(A301-583A, Bethyl); CPSF30(A301-585A, Bethyl); Fip1(A301-462A, Bethyl); CFIm68(A301-356A, Bethyl); These libraries were sequenced on the NovaSeq platform, and all ChIP-seq data processing and analysis were performed according to the ENCODE ChIP-seq pipeline ( https://www.encodeproject.org/data-standards/chip-seq/ ). ..

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: ChIP-seq libraries were prepared using the ChIP-IT® Express Enzymatic Shearing (Active Motif) and ChIP-seq library preparation (Vazyme) kits. .. Primary antibodies used for ChIP included RNAPII (39,497, Active Motif); hnRNPL (18,354-1-AP, Proteintech); RNAPII Ser5 (61,986, Active Motif); RNAPII Ser2 (61,984, Active Motif); LEO1 (PAB14102, Abnova); CFIm68 (A301-358A, Bethyl); CFIm59 (A301-359A, Bethyl); IgG (AC005, Abclonal). .. CUT & Tag libraries for CFIm25 were prepared using the CUT & Tag Hyperactive In-Situ ChIP Library Prep Kit (Vazyme) and primary antibody (10,322-1-AP, Proteintech).

other:

Article Title: The transcriptional terminator XRN2 and the RNA-binding protein Sam68 link alternative polyadenylation to cell cycle progression in prostate cancer
Article Snippet: The following antibodies were used: Sam68 (Bethyl Laboratories, cat. no. A302-110A), XRN2 (Bethyl Laboratories, cat. no. A301-103A), MYC (Cell Signaling, cat. no. 9402), β-actin (Merck, Sigma-Aldrich, cat. no. A2066), CPSF160 (Bethyl Laboratories, cat. no. A301-580A), CPSF100 (Novus, cat. no. NB100-79823), CPSF73 (Bethyl Laboratories, cat. no. A301-091A), CPSF30 (Novus, cat. no. NB100-79826), WDR33 (Bethyl Laboratories, cat. no. A301-152A), CFIM68 (Bethyl Laboratories, cat. no. A301-358A), CSTF50 (Bethyl Laboratories, cat. no. A301-250A), CSTF64 (Bethyl Laboratories, cat. no. A301-092A), PCF11 (Bethyl Laboratories, cat. no. A303-706A), POLR2A (Cell Signaling, cat. no. 14958), H3 (Abcam, cat. no. ab1791), MCM10 (Bethyl Laboratories, cat. no. A300-131A), ORC2 (Bethyl Laboratories, cat. no. A302-734A), Lamin B1 (Santa Cruz, cat. no. sc-30264), GFP (Santa Cruz, cat. no. sc-9996), Flag (Merck, Sigma-Aldrich, cat. no. F3165), BrdU (BD Biosciences, cat. no. 347580) and Alexa-488 (Thermo Fisher Scientific, cat. no. A-11001).

Immunoprecipitation:

Article Title: Identification of Novel Roles for RNA Binding Proteins in pre-mRNA Processing
Article Snippet: .. Endogenous immunoprecipitation was used for Fip1 (Bethyl A301-462A), CPSF100 (Bethyl A301-581A), Symplekin (Bethyl A301-465A), Wdr33 (Bethyl BL4833), and CFIm68 (Bethyl A301-458A). .. 10ug of antibody was incubated with Protein A/G agarose beads (Pierce) for 1hr at room temperature.

Western Blot:

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: .. Western blot assay was conducted using standard techniques, with the following primary antibodies; CFIm25 (10,322-1-AP, Proteintech or sc-81,109, Santa Cruz), REX1 (MA5-38,664, Thermo Scientific), CFIm68 (A301-358A, Bethyl), CFIm59 (A301-359A, Bethyl), GAPDH (sc-32,233, Santa Cruz), Flag (HT201-01, TRANS), His (HT501-01, TRANS). ..

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: .. Western blot assay was conducted using standard techniques, with the following primary antibodies; CFIm25 (10322-1-AP, Proteintech or sc-81109, Santa Cruz), REX1 (MA5-38664, Thermo Scientific), CFIm68 (A301-358A, Bethyl), CFIm59 (A301-359A, Bethyl), GAPDH (sc-32233, Santa Cruz), Flag (HT201-01, TRANS), His (HT501-01, TRANS). ..

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