rabbit polyclonal anti cfim59 (Bethyl)

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Bethyl rabbit polyclonal anti cfim59
Rabbit Polyclonal Anti Cfim59, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfim59/CPSF59+Antibody/pm41741738-758-0-3
Average 93 stars, based on 17 article reviews

rabbit polyclonal anti cfim59 - by Bioz Stars, 2026-09

93/100 stars

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Chromatin Immunoprecipitation:

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: ChIP-seq libraries were prepared using the ChIP-IT® Express Enzymatic Shearing (Active Motif) and ChIP-seq library preparation (Vazyme) kits. .. Primary antibodies used for ChIP included RNAPII (39497, Active Motif); hnRNPL (18354-1-AP, Proteintech); RNAPII Ser5 (61986, Active Motif); RNAPII Ser2 (61984, Active Motif); LEO1 (PAB14102, Abnova); CFIm68 (A301-358A, Bethyl); CFIm59 (A301-359A, Bethyl). .. ChIP-seq libraries for CFIm25 were prepared using the CUT & Tag Hyperactive In-Situ ChIP Library Prep Kit (Vazyme) and primary antibody (10322-1-AP, Proteintech).

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: ChIP-seq libraries were prepared using the ChIP-IT® Express Enzymatic Shearing (Active Motif) and ChIP-seq library preparation (Vazyme) kits. .. Primary antibodies used for ChIP included RNAPII (39,497, Active Motif); hnRNPL (18,354-1-AP, Proteintech); RNAPII Ser5 (61,986, Active Motif); RNAPII Ser2 (61,984, Active Motif); LEO1 (PAB14102, Abnova); CFIm68 (A301-358A, Bethyl); CFIm59 (A301-359A, Bethyl); IgG (AC005, Abclonal). .. CUT & Tag libraries for CFIm25 were prepared using the CUT & Tag Hyperactive In-Situ ChIP Library Prep Kit (Vazyme) and primary antibody (10,322-1-AP, Proteintech).

RNA Immunoprecipitation:

Article Title: U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3’ End Formation
Article Snippet: Proteins were eluted and analyzed by immunoblotting with the following antibodies: U2AF65 (Santa Cruz Biotechnology, MC3), MYC (Roche, 11-667-149-001), β-actin (Sigma, AC74), FLAG M2 (Sigma, F1804). .. RNA immunoprecipitation was performed as described in the Supplemental Experimental Procedures , using the following antibodies: CFIm25 (Santa Cruz, sc-81109), CFIm59 (Bethyl Laboratories, A301-360A) and CFIm68 (Abcam, ab175237) were used for RNA immunoprecipitation and mouse IgG (Abcam, ab18413) or rabbit IgG (Abcam, ab46540) as a negative control. ..

Negative Control:

Western Blot:

Article Title: Herpes simplex virus blocks host transcription termination via the bimodal activities of ICP27
Article Snippet: .. The antibodies for western blotting are as follows: ICP27 (Virusys, P1113 and P1119), CPSF160 (Bethyl, A301-580A), CPSF100 (Bethyl, A301-581A), CPSF73 (Bethyl, A301-091A), CPSF30 (Bethyl, A301-585A), Fip1 (Bethyl, A301-462A), Symplekin (Bethyl, A301-465A), WDR33 (Bethyl, A301-152A), CstF64 (Bethyl, A301-092A), CstF77 (Bethyl, A301-096A), CstF50 (Bethyl, A301-250A), CFIm68 (Bethyl, A201-358A), CFIm59 (Bethyl, A301-360A). ..

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: .. Western blot assay was conducted using standard techniques, with the following primary antibodies; CFIm25 (10,322-1-AP, Proteintech or sc-81,109, Santa Cruz), REX1 (MA5-38,664, Thermo Scientific), CFIm68 (A301-358A, Bethyl), CFIm59 (A301-359A, Bethyl), GAPDH (sc-32,233, Santa Cruz), Flag (HT201-01, TRANS), His (HT501-01, TRANS). ..

Article Title: CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation
Article Snippet: .. Western blot assay was conducted using standard techniques, with the following primary antibodies; CFIm25 (10322-1-AP, Proteintech or sc-81109, Santa Cruz), REX1 (MA5-38664, Thermo Scientific), CFIm68 (A301-358A, Bethyl), CFIm59 (A301-359A, Bethyl), GAPDH (sc-32233, Santa Cruz), Flag (HT201-01, TRANS), His (HT501-01, TRANS). ..

Cross-linking Immunoprecipitation:

Article Title: Regulation of alternative polyadenylation by the C2H2-zinc-finger protein Sp1.
Article Snippet: Article

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