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cfse pi double staining  (MedChemExpress)


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    Structured Review

    MedChemExpress cfse pi double staining
    Cfse Pi Double Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfda-se/CFDA-SE/pm42516037-111-20-23
    Average 97 stars, based on 178 article reviews
    cfse pi double staining - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Cytometry:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Staining:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Software:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Concentration Assay:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Labeling:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Suspension:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Western Blot:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    In Vivo:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    In Vitro:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Plasmid Preparation:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Purification:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    DCFH-DA Assay:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Membrane:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Expressing:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Transfection:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Mouse Assay:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    shRNA:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Isolation:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces

    Suppression Assay:

    Article Title: LAMP5 as a novel prognostic biomarker linked to the tumor immune microenvironment in esophageal squamous cell carcinoma
    Article Snippet: ces, San Jose, CA, USA), and positive CD8 + T cells were analyzed using FlowJo software (v10.8, BD Biosciences). After sorting, CD8 + T cells were washed twice with pre-cooled PBS. CFSE dye (HY-D0938, MedChemExpress) was added to the cell suspension to a final concentration of 5 μM. The cells were labeled at 37°C for 10 min in the dark, and complete medium was added to terminate the staining proces



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    Image Search Results


    FTL008.16 exhibits 5T4-dependent immune cell activation and tumor cell killing in PBMC co-cultures. (a) PBMCs were stimulated with anti-CD3 (0, 10, or 100 ng/mL), and 4-1BB expression on CD4 + T, CD8 + T, and NK cells was analyzed in co-culture with CT26 h5T4 or CT26-WT cells. (b) Concentration-dependent binding of FTL008.16 to anti-CD3/CD28-activated T-cell subsets (CD3 + , CD4 + , CD8 + ) was assessed by flow cytometry. (c) CFSE-labeled PBMCs were stimulated with anti-CD3 and serial dilutions of FTL008.16 or hIgG1 isotype control in the presence of CHO h5T4 cells. CD8 + T-cell counts were assessed by flow cytometry at 96 hours. (d) IFN- γ release from PBMCs and purified T cells was measured after co-culture with 5T4-expressing tumor cells and treatment with FTL008.16 or FTL001 control. (e) Purified T cells and mitomycin C-treated CT26-WT or CT26 h5T4 tumor cells were incubated with serial dilutions of FTL008.16. IFN- γ secretion in supernatants was measured by ELISA. (f) FTL008.16 does not induce cytokine release from PBMCs of healthy donors ( n = 3), as assessed by cytometric bead array (CBA). (g) PBMC-mediated cytotoxicity of FTL001 and FTL008.16 against 5T4-positive (DLD-1) and 5T4-negative (HEK 293T) cells. **p < 0.01, ***p < 0.001 vs. hIgG1 control.

    Journal: Oncoimmunology

    Article Title: FTL008.16, a 5T4-Conditional 4-1BB bispecific antibody, potently enhances antitumor immunity via tumor-directed t-cell activation

    doi: 10.1080/2162402X.2026.2684136

    Figure Lengend Snippet: FTL008.16 exhibits 5T4-dependent immune cell activation and tumor cell killing in PBMC co-cultures. (a) PBMCs were stimulated with anti-CD3 (0, 10, or 100 ng/mL), and 4-1BB expression on CD4 + T, CD8 + T, and NK cells was analyzed in co-culture with CT26 h5T4 or CT26-WT cells. (b) Concentration-dependent binding of FTL008.16 to anti-CD3/CD28-activated T-cell subsets (CD3 + , CD4 + , CD8 + ) was assessed by flow cytometry. (c) CFSE-labeled PBMCs were stimulated with anti-CD3 and serial dilutions of FTL008.16 or hIgG1 isotype control in the presence of CHO h5T4 cells. CD8 + T-cell counts were assessed by flow cytometry at 96 hours. (d) IFN- γ release from PBMCs and purified T cells was measured after co-culture with 5T4-expressing tumor cells and treatment with FTL008.16 or FTL001 control. (e) Purified T cells and mitomycin C-treated CT26-WT or CT26 h5T4 tumor cells were incubated with serial dilutions of FTL008.16. IFN- γ secretion in supernatants was measured by ELISA. (f) FTL008.16 does not induce cytokine release from PBMCs of healthy donors ( n = 3), as assessed by cytometric bead array (CBA). (g) PBMC-mediated cytotoxicity of FTL001 and FTL008.16 against 5T4-positive (DLD-1) and 5T4-negative (HEK 293T) cells. **p < 0.01, ***p < 0.001 vs. hIgG1 control.

    Article Snippet: Human PBMCs were labeled with CFSE (MCE, #HY-D0938) according to the manufacturer’s instructions.

    Techniques: Activation Assay, Expressing, Co-Culture Assay, Concentration Assay, Binding Assay, Flow Cytometry, Labeling, Control, Purification, Incubation, Enzyme-linked Immunosorbent Assay