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Cellenion cellenone dispenser
Cellenone Dispenser, supplied by Cellenion, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellenone/pm42129190-181-9-11
Average 86 stars, based on 1 article reviews
cellenone dispenser - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Single Cell Proteomics Reveals Specific Cellular Subtypes in Cardiomyocytes Derived from Human iPSCs and Adult Hearts.
Article Snippet: Individual aCMs were dispensed into separate wells on the same type of Bio-rad 384-well plate containing same lysis buffer as for iCMs as the using a CellenONE (Cellenion) as previously reported(15).



Article Title: Quantitative measurement of phenotype dynamics during cancer drug resistance evolution using genetic barcoding.
Article Snippet: The barcode oligos in the wells were rehydrated with Lysis and RT buffers followed by cell isolation and dispensing using the cellenONE (Cellenion).

Article Title: Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics.
Article Snippet: Sample preparation with Chip-Tip Sample lysis and digestion is performed within the proteoCHIP EVO 96 inside the cellenONE (Cellenion SASU).

Article Title: Low-resolution FAIMS for increased peptide coverage in low-load and single-cell proteomics
Article Snippet: Cells were resuspended in PBS at a density of 200 cells/μl for isolation using the cellenONE (Cellenion).

Article Title: Challenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity.
Article Snippet: Cells were resuspended in PBS at a density of 200 cells per μl for isolation with the cellenONE (Cellenion).

Cellenone:

Article Title: Neuroblastoma plasticity during metastatic progression stems from the dynamics of an early sympathetic transcriptomic trajectory.
Article Snippet: .. Samples were sorted with the cellenONE technology (Cellenion Bico) to discard non-fluorescent chick cells and to dispatch human single cells in 96-well plates. .. Fulllength RNA-seq was performed with SMART-Seq2 kit according to the standard protocol47 or with the 3’ scRNA-seq seqWell kit (seqWell).



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Cell lines were methanol fixed and permeabilised prior to incubation with Geminin-AF488 antibodies and DAPI staining. Acquisition, analysis and sorting was performed on the BD FACSMelody using the BD FACSChorus™ software. Analysis was performed using Flow Jo v10.10. PEO1 Clone B4 used to demonstrate gating. The gating tree was as follows: a ) FSC-A/SSC-A (gating of cells based on size and intracellular composition) b ) SSC-H/SSC-W and c ) FSC-H/FSC-W for removal of non-singlets d ) DAPI-A/Geminin-AF488-A for cell cycle analysis. Geminin is a DNA replication regulator expressed in S and G2 phase of the cell cycle, and not expressed in G1-phase of the cell cycle. DAPI is used as a DNA content marker, and is routinely used in cell-cycle analysis. Together, geminin and DAPI intensity are used here to gate cells in G1-phase of the cell cycle. The use of a primary-antibody negative control population (AF488 and DAPI staining only) was used to determine the gating of cells with negative expression of geminin. The geminin-negative population were sorted via FACS and subsequent single cell isolation was performed using the <t>cellenONE®</t> cell dispenser.
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Cell lines were methanol fixed and permeabilised prior to incubation with Geminin-AF488 antibodies and DAPI staining. Acquisition, analysis and sorting was performed on the BD FACSMelody using the BD FACSChorus™ software. Analysis was performed using Flow Jo v10.10. PEO1 Clone B4 used to demonstrate gating. The gating tree was as follows: a ) FSC-A/SSC-A (gating of cells based on size and intracellular composition) b ) SSC-H/SSC-W and c ) FSC-H/FSC-W for removal of non-singlets d ) DAPI-A/Geminin-AF488-A for cell cycle analysis. Geminin is a DNA replication regulator expressed in S and G2 phase of the cell cycle, and not expressed in G1-phase of the cell cycle. DAPI is used as a DNA content marker, and is routinely used in cell-cycle analysis. Together, geminin and DAPI intensity are used here to gate cells in G1-phase of the cell cycle. The use of a primary-antibody negative control population (AF488 and DAPI staining only) was used to determine the gating of cells with negative expression of geminin. The geminin-negative population were sorted via FACS and subsequent single cell isolation was performed using the <t>cellenONE®</t> cell dispenser.
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Cell lines were methanol fixed and permeabilised prior to incubation with Geminin-AF488 antibodies and DAPI staining. Acquisition, analysis and sorting was performed on the BD FACSMelody using the BD FACSChorus™ software. Analysis was performed using Flow Jo v10.10. PEO1 Clone B4 used to demonstrate gating. The gating tree was as follows: a ) FSC-A/SSC-A (gating of cells based on size and intracellular composition) b ) SSC-H/SSC-W and c ) FSC-H/FSC-W for removal of non-singlets d ) DAPI-A/Geminin-AF488-A for cell cycle analysis. Geminin is a DNA replication regulator expressed in S and G2 phase of the cell cycle, and not expressed in G1-phase of the cell cycle. DAPI is used as a DNA content marker, and is routinely used in cell-cycle analysis. Together, geminin and DAPI intensity are used here to gate cells in G1-phase of the cell cycle. The use of a primary-antibody negative control population (AF488 and DAPI staining only) was used to determine the gating of cells with negative expression of geminin. The geminin-negative population were sorted via FACS and subsequent single cell isolation was performed using the <t>cellenONE®</t> cell dispenser.
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Cell lines were methanol fixed and permeabilised prior to incubation with Geminin-AF488 antibodies and DAPI staining. Acquisition, analysis and sorting was performed on the BD FACSMelody using the BD FACSChorus™ software. Analysis was performed using Flow Jo v10.10. PEO1 Clone B4 used to demonstrate gating. The gating tree was as follows: a ) FSC-A/SSC-A (gating of cells based on size and intracellular composition) b ) SSC-H/SSC-W and c ) FSC-H/FSC-W for removal of non-singlets d ) DAPI-A/Geminin-AF488-A for cell cycle analysis. Geminin is a DNA replication regulator expressed in S and G2 phase of the cell cycle, and not expressed in G1-phase of the cell cycle. DAPI is used as a DNA content marker, and is routinely used in cell-cycle analysis. Together, geminin and DAPI intensity are used here to gate cells in G1-phase of the cell cycle. The use of a primary-antibody negative control population (AF488 and DAPI staining only) was used to determine the gating of cells with negative expression of geminin. The geminin-negative population were sorted via FACS and subsequent single cell isolation was performed using the cellenONE® cell dispenser.

Journal: bioRxiv

Article Title: scUnique: Measuring Ongoing Chromosomal Instability

doi: 10.64898/2026.04.28.721240

Figure Lengend Snippet: Cell lines were methanol fixed and permeabilised prior to incubation with Geminin-AF488 antibodies and DAPI staining. Acquisition, analysis and sorting was performed on the BD FACSMelody using the BD FACSChorus™ software. Analysis was performed using Flow Jo v10.10. PEO1 Clone B4 used to demonstrate gating. The gating tree was as follows: a ) FSC-A/SSC-A (gating of cells based on size and intracellular composition) b ) SSC-H/SSC-W and c ) FSC-H/FSC-W for removal of non-singlets d ) DAPI-A/Geminin-AF488-A for cell cycle analysis. Geminin is a DNA replication regulator expressed in S and G2 phase of the cell cycle, and not expressed in G1-phase of the cell cycle. DAPI is used as a DNA content marker, and is routinely used in cell-cycle analysis. Together, geminin and DAPI intensity are used here to gate cells in G1-phase of the cell cycle. The use of a primary-antibody negative control population (AF488 and DAPI staining only) was used to determine the gating of cells with negative expression of geminin. The geminin-negative population were sorted via FACS and subsequent single cell isolation was performed using the cellenONE® cell dispenser.

Article Snippet: PEO1 single-cell clones were generated by sorting single cells into 96-well plates containing complete media using the cellenONE® cell dispenser (Scienion).

Techniques: Incubation, Staining, Software, Cell Cycle Assay, Marker, Negative Control, Expressing, Single-cell Isolation, Cellenone

Journal: bioRxiv

Article Title: scUnique: Measuring Ongoing Chromosomal Instability

doi: 10.64898/2026.04.28.721240

Figure Lengend Snippet:

Article Snippet: PEO1 single-cell clones were generated by sorting single cells into 96-well plates containing complete media using the cellenONE® cell dispenser (Scienion).

Techniques: Cellenone, Isolation, Transmission Assay, Selection