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cdnaclones  (ATCC)


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    ATCC cdnaclones
    Cdnaclones, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdnaclones/product/ATCC
    Average 92 stars, based on 20 article reviews
    cdnaclones - by Bioz Stars, 2026-02
    92/100 stars

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    Transcript levels of proton sensing GPCRs ( GPR68, GPR4, GPR65 and GPR132 ) determined by RT-qPCR in ( a ) EndoC-βH2 cells compared with ductal carcinoma SKPC cell-line; ( b ) human islet preparation. Data represented as mean values ± SEM of at least 3 independent experiments. ***p < 0.001; (one-way ANOVA, followed by a Tukey’s multiple comparisons post-test).

    Journal: Scientific Reports

    Article Title: Extracellular acidification stimulates GPR68 mediated IL-8 production in human pancreatic β cells

    doi: 10.1038/srep25765

    Figure Lengend Snippet: Transcript levels of proton sensing GPCRs ( GPR68, GPR4, GPR65 and GPR132 ) determined by RT-qPCR in ( a ) EndoC-βH2 cells compared with ductal carcinoma SKPC cell-line; ( b ) human islet preparation. Data represented as mean values ± SEM of at least 3 independent experiments. ***p < 0.001; (one-way ANOVA, followed by a Tukey’s multiple comparisons post-test).

    Article Snippet: The MGC Human GPR68 cDNAclone (Clone ID: 6971805) was purchased (Open Biosystems; Thermo Scientific) and sub-cloned into pEGFP-N1 (Clonetech).

    Techniques: Quantitative RT-PCR

    EndoC-βH2 cells ( a ) and adult human islets ( b ) were transfected with control non-target siRNA (siNT) or siRNA targeting RFX6 (siRFX6). GPR68 expression was analyzed 72 h post transfection by RT-qPCR. Data are expressed as percentage of siNT transfected cells. (c–e) wtRFX6 and Mut506RFX6 (one-way ANOVA) ( c ), transactivation domain VP16- conjugated RFX6 ( d ) and transcriptional repression domain KRAB- conjugated RFX6 ( e ) were expressed in EndoC-βH2 cells using bicistronic constructs with IRES-EGFP. GFP +ve cells were FACS isolated 48 h post-transfection and GPR68 expression was analyzed by RT-qPCR. Data are mean ± SEM of 3–5 experiments. *p < 0.05; **p < 0.01; ***p < 0.001 and ns , not significant.

    Journal: Scientific Reports

    Article Title: Extracellular acidification stimulates GPR68 mediated IL-8 production in human pancreatic β cells

    doi: 10.1038/srep25765

    Figure Lengend Snippet: EndoC-βH2 cells ( a ) and adult human islets ( b ) were transfected with control non-target siRNA (siNT) or siRNA targeting RFX6 (siRFX6). GPR68 expression was analyzed 72 h post transfection by RT-qPCR. Data are expressed as percentage of siNT transfected cells. (c–e) wtRFX6 and Mut506RFX6 (one-way ANOVA) ( c ), transactivation domain VP16- conjugated RFX6 ( d ) and transcriptional repression domain KRAB- conjugated RFX6 ( e ) were expressed in EndoC-βH2 cells using bicistronic constructs with IRES-EGFP. GFP +ve cells were FACS isolated 48 h post-transfection and GPR68 expression was analyzed by RT-qPCR. Data are mean ± SEM of 3–5 experiments. *p < 0.05; **p < 0.01; ***p < 0.001 and ns , not significant.

    Article Snippet: The MGC Human GPR68 cDNAclone (Clone ID: 6971805) was purchased (Open Biosystems; Thermo Scientific) and sub-cloned into pEGFP-N1 (Clonetech).

    Techniques: Transfection, Expressing, Quantitative RT-PCR, Construct, Isolation

    ( a ) IP formation was determined in EndoC-βH2 cells incubated at pH 7.4 and 6.4. The G q/11 -selective inhibitor compound YM-254890 (100 nM) was also tested. Results are expressed as fold change over the IP values at pH 7.4. ( b ) For cAMP formation assay, EndoC-βH2 cells were incubated for 30 min with and without Forskolin (25 μM) at pH 7.4 or 6.4. Results are expressed as cAMP accumulation (nM) per 5,000 cells. (c) EndoC-βH2 cells were transfected with control siRNA (siNT), siRNA targeting either GPR68 (siGPR68) or RFX6 (siRFX6). 72 h post transfection, cells were incubated at pH 7.4 or 6.4 for 30 min and analyzed for the IP formation. Results are expressed as fold change over the IP values at pH 7.4. Data are mean ± SEM of 3–5 experiments. *p < 0.05; **p < 0.01; ***p < 0.001 and ns , not significant (one-way ANOVA, followed by a Tukey’s multiple comparisons post-test).

    Journal: Scientific Reports

    Article Title: Extracellular acidification stimulates GPR68 mediated IL-8 production in human pancreatic β cells

    doi: 10.1038/srep25765

    Figure Lengend Snippet: ( a ) IP formation was determined in EndoC-βH2 cells incubated at pH 7.4 and 6.4. The G q/11 -selective inhibitor compound YM-254890 (100 nM) was also tested. Results are expressed as fold change over the IP values at pH 7.4. ( b ) For cAMP formation assay, EndoC-βH2 cells were incubated for 30 min with and without Forskolin (25 μM) at pH 7.4 or 6.4. Results are expressed as cAMP accumulation (nM) per 5,000 cells. (c) EndoC-βH2 cells were transfected with control siRNA (siNT), siRNA targeting either GPR68 (siGPR68) or RFX6 (siRFX6). 72 h post transfection, cells were incubated at pH 7.4 or 6.4 for 30 min and analyzed for the IP formation. Results are expressed as fold change over the IP values at pH 7.4. Data are mean ± SEM of 3–5 experiments. *p < 0.05; **p < 0.01; ***p < 0.001 and ns , not significant (one-way ANOVA, followed by a Tukey’s multiple comparisons post-test).

    Article Snippet: The MGC Human GPR68 cDNAclone (Clone ID: 6971805) was purchased (Open Biosystems; Thermo Scientific) and sub-cloned into pEGFP-N1 (Clonetech).

    Techniques: Incubation, Tube Formation Assay, Transfection