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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11022 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11022 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Copper Depletion Nanoparticles Potentiate Cancer Immunotherapy by Avoiding Innate and Adaptive Immune Resistance.
    Article Snippet: .5 Evaluation of Macrophage Phagocytosis of ancer Cells In Vitro B49 and 4T1 tumor cells were labeled with dye CellTrace regon Green 488 (#C34555, ThermoFisher) and incubated with ifferent concentrations of CuCl2 for 8 h. The tumor cells ere further co-cultured with F4-80-labeled (#14-4801-85, TheroFisher) BMDM for 1 h, and the phagocytosis of macrophages as detected by flow cytometry. .. .6 Real-Time Quantitative PCR (RT ‒qPCR) otal cellular RNA was extracted using TRIzol reagent (Invitroen), and cDNA was synthesized by Takara’s reverse transcription it (#RR047A). .. Subsequently, the mRNA expression levels of the arget genes were measured using a real-time quantitative PCR kit #RR820A, Takara, Japan).

    Article Title: Study on the analgesic and anti-inflammatory mechanisms of Jingu Zhitong gel in the treatment of knee osteoarthritis through the IL-17, NGF-TrkA, and COX-2/PGE2 pathways
    Article Snippet: .. The total RNA of synovial tissue and dorsal root ganglion (DRG) was extracted using the TaKaRa MiniBEST Universal RNA Extraction Kit (9767, Takara, China) and reverse-transcribed into cDNA using PrimeScriptTM RT reagent Kit with gDNA Eraser to obtain cDNA (RR047A, Takara, China) for qPCR amplification. qPCR was then performed with TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (RR820Q, Takara, China) on LightCycler®480 Instrument II (Roche, Switzerland). ..

    Article Title: Network toxicology and machine learning revealed the association between non-alcoholic fatty liver disease induced by polystyrene nanoplastics.
    Article Snippet: .. After reverse transcription to synthesize cDNA (Cat.: RR037A, TaKara, Japan), the SYBR Green quantitative PCR kit (Cat.: RR420A, TaKara, Japan) was used according to the instructions, with the addition of primers and relevant reagents. ..

    Synthesized:

    Article Title: Copper Depletion Nanoparticles Potentiate Cancer Immunotherapy by Avoiding Innate and Adaptive Immune Resistance.
    Article Snippet: .5 Evaluation of Macrophage Phagocytosis of ancer Cells In Vitro B49 and 4T1 tumor cells were labeled with dye CellTrace regon Green 488 (#C34555, ThermoFisher) and incubated with ifferent concentrations of CuCl2 for 8 h. The tumor cells ere further co-cultured with F4-80-labeled (#14-4801-85, TheroFisher) BMDM for 1 h, and the phagocytosis of macrophages as detected by flow cytometry. .. .6 Real-Time Quantitative PCR (RT ‒qPCR) otal cellular RNA was extracted using TRIzol reagent (Invitroen), and cDNA was synthesized by Takara’s reverse transcription it (#RR047A). .. Subsequently, the mRNA expression levels of the arget genes were measured using a real-time quantitative PCR kit #RR820A, Takara, Japan).

    Article Title: Dietary supplementation of coconut meat modulates growth performance, nutritional composition, and internal regulation in Chinese mitten crab ( Eriocheir sinensis )
    Article Snippet: .. Six DEGs were selected using the same RNA samples for Illumina transcriptome profiling. cDNA was synthesized utilizing a commercially available kit (#RR047A, Takara). .. Following this, 2 μl of the synthesized cDNA was utilized for the qPCR analysis, employing a commercially available kit (#RR820A, Takara) in conjunction with specific primers.

    Reverse Transcription:

    Article Title: Copper Depletion Nanoparticles Potentiate Cancer Immunotherapy by Avoiding Innate and Adaptive Immune Resistance.
    Article Snippet: .5 Evaluation of Macrophage Phagocytosis of ancer Cells In Vitro B49 and 4T1 tumor cells were labeled with dye CellTrace regon Green 488 (#C34555, ThermoFisher) and incubated with ifferent concentrations of CuCl2 for 8 h. The tumor cells ere further co-cultured with F4-80-labeled (#14-4801-85, TheroFisher) BMDM for 1 h, and the phagocytosis of macrophages as detected by flow cytometry. .. .6 Real-Time Quantitative PCR (RT ‒qPCR) otal cellular RNA was extracted using TRIzol reagent (Invitroen), and cDNA was synthesized by Takara’s reverse transcription it (#RR047A). .. Subsequently, the mRNA expression levels of the arget genes were measured using a real-time quantitative PCR kit #RR820A, Takara, Japan).

    Article Title: Parabacteroides goldsteinii -derived outer membrane vesicles alleviate acute lung injury via modulation of bile acid metabolism
    Article Snippet: Nuclei were counterstained using DAPI (P0131; Beyotime Biotechnology), and images were captured by confocal microscopy. .. RNA was extracted from mouse lung tissue according to the manufacturer’s instructions (RN001; Shanghai Yishan Biotechnology Co. Ltd., Shanghai, China), and cDNA was obtained by reverse transcription (RR036A; Takara Biotechnology, Osaka, Japan). .. The target genes were amplified by TB Green ® Premix Ex Taq TM (Tli RNaseH Plus) (RR420A, Takara Biotechnology).

    Article Title: Study on the analgesic and anti-inflammatory mechanisms of Jingu Zhitong gel in the treatment of knee osteoarthritis through the IL-17, NGF-TrkA, and COX-2/PGE2 pathways
    Article Snippet: .. The total RNA of synovial tissue and dorsal root ganglion (DRG) was extracted using the TaKaRa MiniBEST Universal RNA Extraction Kit (9767, Takara, China) and reverse-transcribed into cDNA using PrimeScriptTM RT reagent Kit with gDNA Eraser to obtain cDNA (RR047A, Takara, China) for qPCR amplification. qPCR was then performed with TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (RR820Q, Takara, China) on LightCycler®480 Instrument II (Roche, Switzerland). ..

    Article Title: Umbilical cord-derived mesenchymal stem cells restore renal homeostasis in Alport Syndrome: Mechanistic insights and clinical translation.
    Article Snippet: 539 After identification tests by TEM, NTA and WB, the EV samples were then used for RNA 540 isolation (miRNeasy® Mini kit 217004, Qiagen, Germany) and purification (RNA Nano 6000 541 Assay Kit 5067-1511, Agilent Technologies, USA) according to the kit instructions, which were 542 analyzed with the Agilent Bioanalyzer System (2100, Agilent Technologies, USA). .. Total RNA 543 was reverse transcribed to produce cDNA (PrimeScriptTM RT reagent Kit RR037A, TAKARA, 544 Japan). ..

    Article Title: Network toxicology and machine learning revealed the association between non-alcoholic fatty liver disease induced by polystyrene nanoplastics.
    Article Snippet: .. After reverse transcription to synthesize cDNA (Cat.: RR037A, TaKara, Japan), the SYBR Green quantitative PCR kit (Cat.: RR420A, TaKara, Japan) was used according to the instructions, with the addition of primers and relevant reagents. ..

    Article Title: Growth Performance and Intestinal Health of Triploid Rainbow Trout ( Oncorhynchus mykiss ) Through Bile Acid Supplementation in Low Fishmeal Diets: Insights From Metabolomics, Microbiota, and Gene Expression
    Article Snippet: .. Total RNA was extracted from the powdered samples using TRIzol Reagent (Ambion, San Diego, CA, USA) and reverse transcribed into cDNA (RR047A; TaKaRa, Beijing, China). .. The concentration and purity of RNA were assessed using the UV colorimetric method (Nanodrop Thermo Fisher Scientific, USA), with absorbance ratios (260/280 nm) of the samples ranging between 1.8 and 2.0.

    Amplification:

    Article Title: Application of nanopore adaptive sampling for metagenomic detection of tick-borne RNA viruses.
    Article Snippet: Extracted RNA was reverse transcribed into cDNA using the SMARTer® PCR cDNA Synthesis Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. .. The resulting cDNA was amplified using the Advantage® 2 PCR Kit (Takara Bio, Shiga, Japan) for 24 cycles, following the manufacturer’s instructions, to obtain sufficient total cDNA for downstream library preparation. .. The amplified products were purified using the Nucleospin Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany) to remove residual primers and enzymes.

    Article Title: Study on the analgesic and anti-inflammatory mechanisms of Jingu Zhitong gel in the treatment of knee osteoarthritis through the IL-17, NGF-TrkA, and COX-2/PGE2 pathways
    Article Snippet: .. The total RNA of synovial tissue and dorsal root ganglion (DRG) was extracted using the TaKaRa MiniBEST Universal RNA Extraction Kit (9767, Takara, China) and reverse-transcribed into cDNA using PrimeScriptTM RT reagent Kit with gDNA Eraser to obtain cDNA (RR047A, Takara, China) for qPCR amplification. qPCR was then performed with TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (RR820Q, Takara, China) on LightCycler®480 Instrument II (Roche, Switzerland). ..

    Polymerase Chain Reaction:

    Article Title: Application of nanopore adaptive sampling for metagenomic detection of tick-borne RNA viruses.
    Article Snippet: Extracted RNA was reverse transcribed into cDNA using the SMARTer® PCR cDNA Synthesis Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. .. The resulting cDNA was amplified using the Advantage® 2 PCR Kit (Takara Bio, Shiga, Japan) for 24 cycles, following the manufacturer’s instructions, to obtain sufficient total cDNA for downstream library preparation. .. The amplified products were purified using the Nucleospin Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany) to remove residual primers and enzymes.

    RNA Extraction:

    Article Title: Study on the analgesic and anti-inflammatory mechanisms of Jingu Zhitong gel in the treatment of knee osteoarthritis through the IL-17, NGF-TrkA, and COX-2/PGE2 pathways
    Article Snippet: .. The total RNA of synovial tissue and dorsal root ganglion (DRG) was extracted using the TaKaRa MiniBEST Universal RNA Extraction Kit (9767, Takara, China) and reverse-transcribed into cDNA using PrimeScriptTM RT reagent Kit with gDNA Eraser to obtain cDNA (RR047A, Takara, China) for qPCR amplification. qPCR was then performed with TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (RR820Q, Takara, China) on LightCycler®480 Instrument II (Roche, Switzerland). ..

    SYBR Green Assay:

    Article Title: Network toxicology and machine learning revealed the association between non-alcoholic fatty liver disease induced by polystyrene nanoplastics.
    Article Snippet: .. After reverse transcription to synthesize cDNA (Cat.: RR037A, TaKara, Japan), the SYBR Green quantitative PCR kit (Cat.: RR420A, TaKara, Japan) was used according to the instructions, with the addition of primers and relevant reagents. ..



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    Image Search Results


    NbLNC2914 acts as a miRNA sponge that inhibits the expression of bmo-miR-2808a-3p.

    Journal: Engineering Microbiology

    Article Title: Cross-kingdom noncoding RNA regulation facilitates Nosema bombycis proliferation

    doi: 10.1016/j.engmic.2026.100278

    Figure Lengend Snippet: NbLNC2914 acts as a miRNA sponge that inhibits the expression of bmo-miR-2808a-3p.

    Article Snippet: Next, the total RNAs were used for cDNA synthesis with the PrimeScriptTM RT reagent Kit (Takara, Japan) and miRNA first-strand cDNA synthesis (Tailing Reaction) (Sangon Biotech, China).

    Techniques: Expressing