cd21 (Bio-Rad)
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Cd21, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd21/Mouse+anti+Canine+CD21/pmc13129212-54-47-51
Average 93 stars, based on 91 article reviews
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Saline:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Centrifugation:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Staining:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Article Title: Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs Article Snippet: After five days of stimulation, cells were harvested and stained with canine Fc block (14-9162-42, ThermoFisher Scientific) for 10 minutes prior to the addition of CD45RA antibody (CA4.1D3, unconjugated, BioRad) for 15 minutes. .. Cells were then washed with sorter buffer, centrifuged and stained at 4°C for 20 minutes with an anti-mouse IgG1 (M1-14D12, Super Bright 780, Invitrogen) as a secondary antibody to detect CD45RA + cells, along with other antibodies directed against CD62L (FMC46, StarBright UltraViolet 605, BioRad), CD4 (YKIX302.9, PE-Cy7, BioRad), CD8 (YCATE55.9, Pacific Blue, BioRad), CD11b (M1/70, FITC, BioLegend), Article Title: Increased Abundance of Cytotoxic T Cells in Synovial Fluid of a Dog With Erosive Immune‐Mediated Polyarthritis Article Snippet: Prepared cell samples were incubated for 10 min with an Fc receptor–binding inhibitor polyclonal antibody (14‐9162‐42; eBioscience, San Diego, California, United States) to prevent nonspecific staining. .. Peripheral blood and synovial fluid were stained for 20 min on ice with fluorescent dye–conjugated monoclonal antibodies specific for the following markers: CD45 (clone YKIX716.13, 48‐5450‐42; eBioscience), CD11b (clone M1/70, 101216; BioLegend, San Diego, California, United States), CD14 (clone TÜK4, MCA1568A647; Bio‐Rad Laboratories, Hercules, California, United States), Article Title: Rabies vaccination induces a CD4+ TEM and CD4+CD8+ TEMRA TH1 phenotype in dogs. Article Snippet: After five days of stimulation, cells were harvested and stained with canine Fc block (14-9162-42, ThermoFisher Scientific) for 10 minutes prior to the addition of CD45RA antibody (CA4.1D3, unconjugated, BioRad) for 15 minutes. .. Cells were then washed with sorter buffer, centrifuged and stained at 4°C for 20 minutes with an anti-mouse IgG1 (M1-14D12, Super Bright 780, Invitrogen) as a secondary antibody to detect CD45RA+ cells, along with other antibodies directed against CD62L (FMC46, StarBright UltraViolet 605, BioRad), CD4 (YKIX302.9, PE-Cy7, BioRad), CD8 (YCATE55.9, Pacific Blue, BioRad), CD11b (M1/70, FITC, BioLegend), Bioprocessing:Article Title: Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves Article Snippet: Blood samples (100 μL) were incubated with VersaLyse (Beckman Coulter GmbH, Krefeld, Germany) for 20 min at room temperature and centrifuged at 250 × g for 5 min. .. Obtained cell pellets were washed twice by adding 700 μL phosphate-buffered saline (PBS) and subsequent centrifugation at 150 × g for 5 min. Staining of leukocytes was reached by adding monoclonal antibodies for marking CD4 (co-receptor primarily expressed on T-helper cells, monocytes, and macrophages; mouse anti-bovine CD4: +FITC; Bio-Rad, Hercules, USA), CD8 (co-receptor primarily expressed on cytotoxic T cells; mouse anti-bovine CD8: +RPE; Bio-Rad), CD14 (receptor expressed on monocytes and macrophages; mouse anti-bovine CD14: +RPE; Bio-Rad), and Article Title: Increased Abundance of Cytotoxic T Cells in Synovial Fluid of a Dog With Erosive Immune‐Mediated Polyarthritis Article Snippet: Prepared cell samples were incubated for 10 min with an Fc receptor–binding inhibitor polyclonal antibody (14‐9162‐42; eBioscience, San Diego, California, United States) to prevent nonspecific staining. .. Peripheral blood and synovial fluid were stained for 20 min on ice with fluorescent dye–conjugated monoclonal antibodies specific for the following markers: CD45 (clone YKIX716.13, 48‐5450‐42; eBioscience), CD11b (clone M1/70, 101216; BioLegend, San Diego, California, United States), CD14 (clone TÜK4, MCA1568A647; Bio‐Rad Laboratories, Hercules, California, United States), other:Article Title: High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats Article Snippet: CD21 , CA2.1D6 , Article Title: High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats Article Snippet: CD21 , CA2.1D6 , Article Title: Inflammatory and immune variables as predictors of survival in dogs with myxomatous mitral valve disease. Article Snippet: Monoclonal rat and mouse anti-canine antibodies against CD3 (clone CA17.2A12), CD4 (clone YKIX302.9), CD8 (clone YCATE55.9) in a mix (CD3:FITC/CD4:RPE/ CD8:Alexa Fluor 647, ref: TC014), CD45 (clone YKIX716.13; Alexa Fluor 488, ref: |

