Journal: Physiological Reports
Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10
doi: 10.14814/phy2.70891
Figure Lengend Snippet: (a) Representative immunoreactivities CD68 of a marker of macrophage in the lamina propria of rat jejunal villi without (control) and with the clodronate treatment. (b) Representative immunoreactivities of F4/80, a marker of macrophage in the lamina propria of rat jejunal villi without (control) and with the clodronate treatment. (c) Representative immunoreactivities of CD169, a marker of macrophage in the lamina propria of rat jejunal villi without (control) and with the clodronate treatment. (d–f) Summarized data of density analyses of CD69, F4/80, and CD169, respectively. The error bars represent SDs.
Article Snippet: The fixed samples were sectioned, and the slices were washed three times with PBS and incubated for 2 h at room temperature with primary polyclonal antibodies to FITC‐labeled CD68 (catalog no. bs‐06449R, Bioss, Boston, USA), F4/80 (catalog no. 28463‐1‐AP, Proteintech, Rosemont, USA), or CD169 (catalog no. bs‐10751R, Bioss, Boston, USA), all diluted to 1:5000.
Techniques: Marker, Control