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cd1530  (MedChemExpress)


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    MedChemExpress cd1530
    a , Schematic of the strategy used for identifying chemical cocktails that supports generation of transiently induced totipotent-like cells. Td, tdTomato. b , Results of the primary chemical screen using mouse EPSCs carrying the MuERV-L -mClover3 reporter. The dashed line indicates the threshold, which is set to two-fold changes. The values were normalized based on log2. n = 3 biological replicates. c , Heatmaps showing the upregulation of totipotent marker genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CE, CHIR-99021+Elvitegravir; CP, CHIR-99021 + PD0325901; CCD, CHIR-99021 + <t>CD1530;</t> S1, CD1530 + PD0325901+Elvitegravir+CHIR-99021. d , Heatmaps showing the upregulation of primitive endoderm genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CCD, CHIR-99021 + CD1530. e , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical totipotent marker genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.31 × 10 −7 , 6.67 × 10 −8 , 1.64 × 10 −7 , 2.04 × 10 −7 , 1.40 × 10 −3 , 7.98 × 10 −5 , 1.89 × 10 −2 . f , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical primitive endoderm genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. *** p < 0.001; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.29 × 10 −5 , 8.05 × 10 −11 , 4.60 × 10 −14 , 8.70 × 10 −4 , 0.117. g , Cell doubling times of CCD, +PD, +PD + E(S1), EPSCs and TPSC. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901; +PD + E (S1), CHIR-99021 + CD1530 + PD0325901+Elvitegravir. Data are presented as mean ± SD, n = 3 biological replicates. **** p < 0.0001 (One-way ANOVA). p values from left to right: 3.35 × 10 −5 , 1.85 × 10 −6 , 3.10 × 10 −11 .
    Cd1530, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation"

    Article Title: A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation

    Journal: Nature Cell Biology

    doi: 10.1038/s41556-025-01793-9

    a , Schematic of the strategy used for identifying chemical cocktails that supports generation of transiently induced totipotent-like cells. Td, tdTomato. b , Results of the primary chemical screen using mouse EPSCs carrying the MuERV-L -mClover3 reporter. The dashed line indicates the threshold, which is set to two-fold changes. The values were normalized based on log2. n = 3 biological replicates. c , Heatmaps showing the upregulation of totipotent marker genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CE, CHIR-99021+Elvitegravir; CP, CHIR-99021 + PD0325901; CCD, CHIR-99021 + CD1530; S1, CD1530 + PD0325901+Elvitegravir+CHIR-99021. d , Heatmaps showing the upregulation of primitive endoderm genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CCD, CHIR-99021 + CD1530. e , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical totipotent marker genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.31 × 10 −7 , 6.67 × 10 −8 , 1.64 × 10 −7 , 2.04 × 10 −7 , 1.40 × 10 −3 , 7.98 × 10 −5 , 1.89 × 10 −2 . f , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical primitive endoderm genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. *** p < 0.001; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.29 × 10 −5 , 8.05 × 10 −11 , 4.60 × 10 −14 , 8.70 × 10 −4 , 0.117. g , Cell doubling times of CCD, +PD, +PD + E(S1), EPSCs and TPSC. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901; +PD + E (S1), CHIR-99021 + CD1530 + PD0325901+Elvitegravir. Data are presented as mean ± SD, n = 3 biological replicates. **** p < 0.0001 (One-way ANOVA). p values from left to right: 3.35 × 10 −5 , 1.85 × 10 −6 , 3.10 × 10 −11 .
    Figure Legend Snippet: a , Schematic of the strategy used for identifying chemical cocktails that supports generation of transiently induced totipotent-like cells. Td, tdTomato. b , Results of the primary chemical screen using mouse EPSCs carrying the MuERV-L -mClover3 reporter. The dashed line indicates the threshold, which is set to two-fold changes. The values were normalized based on log2. n = 3 biological replicates. c , Heatmaps showing the upregulation of totipotent marker genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CE, CHIR-99021+Elvitegravir; CP, CHIR-99021 + PD0325901; CCD, CHIR-99021 + CD1530; S1, CD1530 + PD0325901+Elvitegravir+CHIR-99021. d , Heatmaps showing the upregulation of primitive endoderm genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CCD, CHIR-99021 + CD1530. e , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical totipotent marker genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.31 × 10 −7 , 6.67 × 10 −8 , 1.64 × 10 −7 , 2.04 × 10 −7 , 1.40 × 10 −3 , 7.98 × 10 −5 , 1.89 × 10 −2 . f , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical primitive endoderm genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. *** p < 0.001; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.29 × 10 −5 , 8.05 × 10 −11 , 4.60 × 10 −14 , 8.70 × 10 −4 , 0.117. g , Cell doubling times of CCD, +PD, +PD + E(S1), EPSCs and TPSC. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901; +PD + E (S1), CHIR-99021 + CD1530 + PD0325901+Elvitegravir. Data are presented as mean ± SD, n = 3 biological replicates. **** p < 0.0001 (One-way ANOVA). p values from left to right: 3.35 × 10 −5 , 1.85 × 10 −6 , 3.10 × 10 −11 .

    Techniques Used: Marker, Expressing

    a , Pseudotime trajectory reconstruction of single cells from subpopulations at S1 and S2. b , Box plots showing the expression of genes enriched in subpopulation 1 at S1 (S1-1) across subpopulations from S1 and S2. Each box depicts the median level along with the first and third quartiles. The whiskers represent the minimum and maximum values. Points indicate outliers, which are defined as values exceeding 1.5 times the interquartile range from the box. The dots in the boxplot represent genes. p value was calculated using the two-sided Wilcoxon rank-sum test, n = 100 (number of genes). p values from left to right: 2.96 × 10 −17 , 3.04 × 10 −22 , 2.16 × 10 −27 , 7.28 × 10 −24 . c , Heatmaps showing the expression of totipotency marker genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. d , Heatmaps showing the expression of Nr5a2 and Tfap2c target genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. e , Expression of marker genes for epiblast, trophectoderm and primitive endoderm in S3 cell aggregates under different chemical combinations. S3, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530.
    Figure Legend Snippet: a , Pseudotime trajectory reconstruction of single cells from subpopulations at S1 and S2. b , Box plots showing the expression of genes enriched in subpopulation 1 at S1 (S1-1) across subpopulations from S1 and S2. Each box depicts the median level along with the first and third quartiles. The whiskers represent the minimum and maximum values. Points indicate outliers, which are defined as values exceeding 1.5 times the interquartile range from the box. The dots in the boxplot represent genes. p value was calculated using the two-sided Wilcoxon rank-sum test, n = 100 (number of genes). p values from left to right: 2.96 × 10 −17 , 3.04 × 10 −22 , 2.16 × 10 −27 , 7.28 × 10 −24 . c , Heatmaps showing the expression of totipotency marker genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. d , Heatmaps showing the expression of Nr5a2 and Tfap2c target genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. e , Expression of marker genes for epiblast, trophectoderm and primitive endoderm in S3 cell aggregates under different chemical combinations. S3, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530.

    Techniques Used: Expressing, Marker



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    a , Schematic of the strategy used for identifying chemical cocktails that supports generation of transiently induced totipotent-like cells. Td, tdTomato. b , Results of the primary chemical screen using mouse EPSCs carrying the MuERV-L -mClover3 reporter. The dashed line indicates the threshold, which is set to two-fold changes. The values were normalized based on log2. n = 3 biological replicates. c , Heatmaps showing the upregulation of totipotent marker genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CE, CHIR-99021+Elvitegravir; CP, CHIR-99021 + PD0325901; CCD, CHIR-99021 + <t>CD1530;</t> S1, CD1530 + PD0325901+Elvitegravir+CHIR-99021. d , Heatmaps showing the upregulation of primitive endoderm genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CCD, CHIR-99021 + CD1530. e , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical totipotent marker genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.31 × 10 −7 , 6.67 × 10 −8 , 1.64 × 10 −7 , 2.04 × 10 −7 , 1.40 × 10 −3 , 7.98 × 10 −5 , 1.89 × 10 −2 . f , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical primitive endoderm genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. *** p < 0.001; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.29 × 10 −5 , 8.05 × 10 −11 , 4.60 × 10 −14 , 8.70 × 10 −4 , 0.117. g , Cell doubling times of CCD, +PD, +PD + E(S1), EPSCs and TPSC. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901; +PD + E (S1), CHIR-99021 + CD1530 + PD0325901+Elvitegravir. Data are presented as mean ± SD, n = 3 biological replicates. **** p < 0.0001 (One-way ANOVA). p values from left to right: 3.35 × 10 −5 , 1.85 × 10 −6 , 3.10 × 10 −11 .
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    Image Search Results


    a , Schematic of the strategy used for identifying chemical cocktails that supports generation of transiently induced totipotent-like cells. Td, tdTomato. b , Results of the primary chemical screen using mouse EPSCs carrying the MuERV-L -mClover3 reporter. The dashed line indicates the threshold, which is set to two-fold changes. The values were normalized based on log2. n = 3 biological replicates. c , Heatmaps showing the upregulation of totipotent marker genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CE, CHIR-99021+Elvitegravir; CP, CHIR-99021 + PD0325901; CCD, CHIR-99021 + CD1530; S1, CD1530 + PD0325901+Elvitegravir+CHIR-99021. d , Heatmaps showing the upregulation of primitive endoderm genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CCD, CHIR-99021 + CD1530. e , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical totipotent marker genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.31 × 10 −7 , 6.67 × 10 −8 , 1.64 × 10 −7 , 2.04 × 10 −7 , 1.40 × 10 −3 , 7.98 × 10 −5 , 1.89 × 10 −2 . f , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical primitive endoderm genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. *** p < 0.001; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.29 × 10 −5 , 8.05 × 10 −11 , 4.60 × 10 −14 , 8.70 × 10 −4 , 0.117. g , Cell doubling times of CCD, +PD, +PD + E(S1), EPSCs and TPSC. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901; +PD + E (S1), CHIR-99021 + CD1530 + PD0325901+Elvitegravir. Data are presented as mean ± SD, n = 3 biological replicates. **** p < 0.0001 (One-way ANOVA). p values from left to right: 3.35 × 10 −5 , 1.85 × 10 −6 , 3.10 × 10 −11 .

    Journal: Nature Cell Biology

    Article Title: A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation

    doi: 10.1038/s41556-025-01793-9

    Figure Lengend Snippet: a , Schematic of the strategy used for identifying chemical cocktails that supports generation of transiently induced totipotent-like cells. Td, tdTomato. b , Results of the primary chemical screen using mouse EPSCs carrying the MuERV-L -mClover3 reporter. The dashed line indicates the threshold, which is set to two-fold changes. The values were normalized based on log2. n = 3 biological replicates. c , Heatmaps showing the upregulation of totipotent marker genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CE, CHIR-99021+Elvitegravir; CP, CHIR-99021 + PD0325901; CCD, CHIR-99021 + CD1530; S1, CD1530 + PD0325901+Elvitegravir+CHIR-99021. d , Heatmaps showing the upregulation of primitive endoderm genes in mouse EPSCs under different chemical combinations. Samples were treated for three days before their collection. C, CHIR-99021; CCD, CHIR-99021 + CD1530. e , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical totipotent marker genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.31 × 10 −7 , 6.67 × 10 −8 , 1.64 × 10 −7 , 2.04 × 10 −7 , 1.40 × 10 −3 , 7.98 × 10 −5 , 1.89 × 10 −2 . f , Q-PCR analysis showing the effect of PD0325901 on the expression levels of classical primitive endoderm genes. Samples were treated for three days before their collection. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901. Data are presented as mean ± SEM, n = 3 biological replicates. *** p < 0.001; **** p < 0.0001; ns, non-significant (Two-way ANOVA). p values from left to right: 3.29 × 10 −5 , 8.05 × 10 −11 , 4.60 × 10 −14 , 8.70 × 10 −4 , 0.117. g , Cell doubling times of CCD, +PD, +PD + E(S1), EPSCs and TPSC. CCD, CHIR-99021 + CD1530; +PD, CHIR-99021 + CD1530 + PD0325901; +PD + E (S1), CHIR-99021 + CD1530 + PD0325901+Elvitegravir. Data are presented as mean ± SD, n = 3 biological replicates. **** p < 0.0001 (One-way ANOVA). p values from left to right: 3.35 × 10 −5 , 1.85 × 10 −6 , 3.10 × 10 −11 .

    Article Snippet: The totipotency medium consisted of 1640 basal medium supplemented with elvitegravir (1 μM, MCE, HY-14740), CHIR-99021 (3 μM, Selleck, S1263), CD1530 (0.2 μM, MCE, HY-108527) and PD0325901(0.5 μM, Selleck, S1036).

    Techniques: Marker, Expressing

    a , Pseudotime trajectory reconstruction of single cells from subpopulations at S1 and S2. b , Box plots showing the expression of genes enriched in subpopulation 1 at S1 (S1-1) across subpopulations from S1 and S2. Each box depicts the median level along with the first and third quartiles. The whiskers represent the minimum and maximum values. Points indicate outliers, which are defined as values exceeding 1.5 times the interquartile range from the box. The dots in the boxplot represent genes. p value was calculated using the two-sided Wilcoxon rank-sum test, n = 100 (number of genes). p values from left to right: 2.96 × 10 −17 , 3.04 × 10 −22 , 2.16 × 10 −27 , 7.28 × 10 −24 . c , Heatmaps showing the expression of totipotency marker genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. d , Heatmaps showing the expression of Nr5a2 and Tfap2c target genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. e , Expression of marker genes for epiblast, trophectoderm and primitive endoderm in S3 cell aggregates under different chemical combinations. S3, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530.

    Journal: Nature Cell Biology

    Article Title: A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation

    doi: 10.1038/s41556-025-01793-9

    Figure Lengend Snippet: a , Pseudotime trajectory reconstruction of single cells from subpopulations at S1 and S2. b , Box plots showing the expression of genes enriched in subpopulation 1 at S1 (S1-1) across subpopulations from S1 and S2. Each box depicts the median level along with the first and third quartiles. The whiskers represent the minimum and maximum values. Points indicate outliers, which are defined as values exceeding 1.5 times the interquartile range from the box. The dots in the boxplot represent genes. p value was calculated using the two-sided Wilcoxon rank-sum test, n = 100 (number of genes). p values from left to right: 2.96 × 10 −17 , 3.04 × 10 −22 , 2.16 × 10 −27 , 7.28 × 10 −24 . c , Heatmaps showing the expression of totipotency marker genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. d , Heatmaps showing the expression of Nr5a2 and Tfap2c target genes in S2 cell aggregates under different chemical combinations. S2, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530. e , Expression of marker genes for epiblast, trophectoderm and primitive endoderm in S3 cell aggregates under different chemical combinations. S3, CHIR-99021+Birabresib+CD1530 + 8-Br-cAMP; -C, Birabresib+CD1530 + 8-Br-cAMP; -B, CHIR-99021 + CD1530 + 8-Br-cAMP; -CD, CHIR-99021+Birabresib+8-Br-cAMP; -8Br, CHIR-99021+Birabresib+CD1530.

    Article Snippet: The totipotency medium consisted of 1640 basal medium supplemented with elvitegravir (1 μM, MCE, HY-14740), CHIR-99021 (3 μM, Selleck, S1263), CD1530 (0.2 μM, MCE, HY-108527) and PD0325901(0.5 μM, Selleck, S1036).

    Techniques: Expressing, Marker