cd151  (Proteintech)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: CD151 interacts with integrin beta 2 in B cell lymphomas

doi: 10.1007/s00018-025-05747-0

Figure Lengend Snippet: CD151 is expressed by human B cells and B cell lymphomas. a Protein expression of CD151 was investigated with surface staining of PBMCs with clone 11G5a and gated for naïve (CD3 − , CD19 + , CD27 − ) and memory B cells (CD3 − , CD19 + , CD27 + ) using flow cytometry. b Quantification of panel a: Median fluorescence intensity (MdFI) of CD151 plasma membrane expression on memory and naïve B cells from PBMCs ( N = 3). c Tonsillar lymphocyte were gated for naïve (CD3 − , CD19 + , CD27 − , CD38 − ), memory (CD3 − , CD19 + , CD27 + , CD38 − ), germinal center B cells (CD3 − , CD19 + , CD27 low , CD38 + ), and plasma cells (CD3 − , CD19 + , CD27 high , CD38 high ). d Quantification of panel c: MdFI of CD151 plasma membrane expression on tonsillar B cells ( N = 3). e mRNA expression of CD151 and CD20 in B cell lymphoma cell lines from the DepMap project. Cell lines are distinguished by color-coded disease classification: Burkitt lymphoma (dark blue), diffuse large B-cell lymphoma (green), other B cell lymphomas (yellow). f Expression of CD151 on selected B cell lymphoma cell lines stained with biotinylated CD151 antibody clone 11G5a (black points) compared to isotype control (grey points) ( N = 2). g Normalized mRNA expression of CD151 in different primary lymphomas (DLBCL, FL, BL) and normal B cells compared to CD20 expression. Red line indicates expression in normal control. Data from German MMML consortium (see method section)

Article Snippet: Expression plasmids containing wildtype CD151 and N- and C-terminally tagged CD151 were purchased form GenScript (# NM_004357.5 ).

Techniques: Expressing, Staining, Flow Cytometry, Fluorescence, Clinical Proteomics, Membrane, Control

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: CD151 interacts with integrin beta 2 in B cell lymphomas

doi: 10.1007/s00018-025-05747-0

Figure Lengend Snippet: CD151 interacts with ITGB2. a Generation of BJAB cell lines deficient for CD151 (CD151KO) and over expressing GFP-tagged CD151. Histogram of surface stained CD151 (antibody clone 11G5a) using flow cytometry. b Volcano plot of proteome data. Enrichment of CD151 pulldown over control beads is shown with log2 scaling and the false discovery rate of performed t-test as -log 10. CD151 is marked dark grey, integrins and B cell marker proteins grey. N = 3, one representative experiment of three shown. Full list can be found in Suppl. Table . c Pulldown (IP) of CD151 and ITGB2 in lysates from BJAB cells transiently transfected with ALFA-tagged ITGB2 and GFP-tagged CD151. Western blots were stained for ALFA-tag and GFP to visualize ITGB2 (80 kDa) and CD151-GFP (50 kDa), respectively (N = 2). d Pulldown (IP) of GFP-tagged CD151 in lysates from BJAB cells (WT and CD151-GFP) showing CD151 interaction with endogenous ITGB2. Western blots were stained for ITGB2 (80 kDa) and CD151-GFP (50 kDa) (N = 3)

Article Snippet: Expression plasmids containing wildtype CD151 and N- and C-terminally tagged CD151 were purchased form GenScript (# NM_004357.5 ).

Techniques: Expressing, Staining, Flow Cytometry, Control, Marker, Transfection, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: CD151 interacts with integrin beta 2 in B cell lymphomas

doi: 10.1007/s00018-025-05747-0

Figure Lengend Snippet: CD151 depletion affects expression of integrin alpha subunits and cell spreading. a Co-localization and correlation analysis of GFP-tagged CD151 with ITGB2, isotype (negative control) and CD151 (positive control). Representative images are shown, Pearson’s coefficients of the analyzed images are shown in the plot on the right. (Magnification 63X, scale bar = 10 µm) b Surface expression of ITGAL, ITGAM, and ITGAX was determined by flow cytometry on BJAB cell lines, one representative image is shown. Variation in surface expression between WT and CD151KO cells was determined by means of a two-tailed paired student’s t-test mean ± SD (* P < 0.05), shown on the right (N = 3). gMFI = geometric mean fluorescence intensity. c Western blot of alpha chains in lysates from BJAB WT and BJAB CD151KO cells. Blots were stained for ITGAL (180 kDa), ITGAM (165 kDa), ITGAX (145 kDa), CD151 (28 kDa), and ITGB2 (80 kDa). Vinculin (124 kDa) was used as loading control (N = 3). d Cell spreading of BJAB WT and BJAB CD151KO cells on ICAM-1 coating was determined using Airyscan confocal microscopy. Representative images are shown, cell area and perimeter are shown in the plots below. Statistical significance was assessed by Mann–Whitney U test (**** P < 0.0001). (Magnification 63X, scale bar = 10 µm, N = 3, WT = 146 cells, CD151KO = 148 cells)

Article Snippet: Expression plasmids containing wildtype CD151 and N- and C-terminally tagged CD151 were purchased form GenScript (# NM_004357.5 ).

Techniques: Expressing, Negative Control, Positive Control, Flow Cytometry, Two Tailed Test, Fluorescence, Western Blot, Staining, Control, Confocal Microscopy, MANN-WHITNEY

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: CD151 interacts with integrin beta 2 in B cell lymphomas

doi: 10.1007/s00018-025-05747-0

Figure Lengend Snippet: Integrin-free CD151 is the predominant form of CD151 on primary B cells and B cell lymphomas. a Representative flow cytometry plots for CD151 stained naive (CD3 − , CD19 + , CD27 − ) and memory B (CD3 − , CD19 + , CD27 + ) and CD4 T cells (CD3 + , CD4 + ) from PBMCs (N = 2). Samples were stained with CD151 antibody clones 11G5a or 50–6 and isotype control. For B cells, CD151 expression is plotted against CD27 expression to differentiate between naive and memory B cells. For CD4 T cells, CD151 expression was plotted against CD38. b Quantification of percentage of CD151-positive memory B cells. c Quantification of percentage of CD151-positive CD4 T cells. d B cell lymphoma cell lines with varying CD151 expression (BJAB with over-expressed GFP-tagged CD151) were stained with CD151 antibody clones 11G5a or 50–6 (filled histograms) and isotype control (open histograms)

Article Snippet: Expression plasmids containing wildtype CD151 and N- and C-terminally tagged CD151 were purchased form GenScript (# NM_004357.5 ).

Techniques: Flow Cytometry, Staining, Clone Assay, Control, Expressing

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: CD151 interacts with integrin beta 2 in B cell lymphomas

doi: 10.1007/s00018-025-05747-0

Figure Lengend Snippet: Expression and prognostic effect of CD151 antigen and integrins in patients with DLBCL. a Representative CD151 IHC staining with clone E4I9J and isotype control on DLBCL tissues (TMA slides from ). b Protein expression of CD151 was correlated with ABC DLBCL subtype in CD151 positive samples. c Protein expression of CD151 is not a prognostic marker for overall survival (OS) or progression-free survival (PFS) in DLBCL patients (N = 196). d OS of CD151 binding partners from IP studies showed integrins as prognostic marker for OS of DLBCL patients: OS was significantly correlated with tissue mRNA expression of ITGA3, ITGB2, ITGAL, and ITGAM. Data was obtained from GSE31312 , R-CHOP treated DLBCL (N = 498)

Article Snippet: Expression plasmids containing wildtype CD151 and N- and C-terminally tagged CD151 were purchased form GenScript (# NM_004357.5 ).

Techniques: Expressing, Immunohistochemistry, Control, Marker, Binding Assay