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Proteintech cd147
Cd147, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd147/CD147+Antibody/pm41862645-194-39-40
Average 94 stars, based on 70 article reviews
cd147 - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

Cell Culture:

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 min with 0.2% Triton X-100, blocked with 10% goat serum for 30 min, and then incubated overnight at 4 °C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer.
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 minutes with 0.2% Triton X-100, blocked with 10% goat serum for 30 minutes, and then incubated overnight at 4°C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Adhesive:

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 min with 0.2% Triton X-100, blocked with 10% goat serum for 30 min, and then incubated overnight at 4 °C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer.
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 minutes with 0.2% Triton X-100, blocked with 10% goat serum for 30 minutes, and then incubated overnight at 4°C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Incubation:

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 min with 0.2% Triton X-100, blocked with 10% goat serum for 30 min, and then incubated overnight at 4 °C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer
Article Snippet: .. Briefly, the supernatant was incubated with 30 μg of primary antibodies to CD147, MCT1 (20139-1-AP, Proteintech), or LDHB (14824-1-AP, Proteintech) at 4 °C overnight, with irrelevant mouse IgG (P5000-Y, GENIA) or rabbit IgG (I8140, Sigma) used as negative controls. .. The following day, Protein A&G beads (Invitrogen) were added and incubated at 4 °C for 4 h. The immuno-precipitates were then eluted and identified by western blot, with the isolated mitochondria used as an input control.

Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Article Snippet: 14 The EMBO Journal © The Author(s) transfer to PVDF (Fisher Scientific) membrane. .. The blots were incubated in 2% BSA/Tri-buffered saline tween-20 (TBST) overnight at 4 °C with primary antibodies specifically for: HSP90 (Santa Cruz, #sc-13119, 1:5000), SEL1L (home-made, 1:10,000) (Zhou et al, 2020), SEL1L (Abcam, ab78298, 1:1000), HRD1 (Proteintech, #13473-1, 1:2000), CD147 (Proteintech, #11989-1, 1:3000), IRE1α (Cell Signaling, #3294, 1:2000), ERLEC1 (Abcam, #ab181166, 1:5000), BiP/GRP94 (Abcam, #ab21685, 1:5000), PDI (Enzo, #ADI-SPA-890, 1:5000), PERK (Cell Signaling, #3192, 1:5000). .. Membranes were washed with TBST and incubated with secondary antibodies, either HRP conjugated (Bio-Rad, 1:10,000), anti-Rabbit IgG TrueBlot HRP (Rockland, #18-8816-33, 1:500) or anti-Mouse IgG TrueBlot-HRP (Rockland, #18-8817-31, 1:500) at room temperature for 1 h for ECL chemiluminescence detection system (Bio-Rad) development.

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer.
Article Snippet: .. Briefly, the supernatant was incubated with 30 μg of primary antibodies to CD147, MCT1 (20139-1-AP, Proteintech), or LDHB (14824-1-AP, Proteintech) at 4 °C overnight, with irrelevant mouse IgG (P5000Y, GENIA) or rabbit IgG (I8140, Sigma) used as negative controls. .. The following day, Protein A&G beads (Invitrogen) were added and incubated at 4 °C for 4 hours.

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer.
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 minutes with 0.2% Triton X-100, blocked with 10% goat serum for 30 minutes, and then incubated overnight at 4°C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Methylation:

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 min with 0.2% Triton X-100, blocked with 10% goat serum for 30 min, and then incubated overnight at 4 °C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer.
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 minutes with 0.2% Triton X-100, blocked with 10% goat serum for 30 minutes, and then incubated overnight at 4°C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Marker:

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 min with 0.2% Triton X-100, blocked with 10% goat serum for 30 min, and then incubated overnight at 4 °C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer.
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 minutes with 0.2% Triton X-100, blocked with 10% goat serum for 30 minutes, and then incubated overnight at 4°C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Binding Assay:

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 min with 0.2% Triton X-100, blocked with 10% goat serum for 30 min, and then incubated overnight at 4 °C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Article Title: Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer.
Article Snippet: In lactate-treated cells, the DMEM/F12 medium was replaced with glucose-free DMEM/F12 medium (Procell, Canton, MA, USA), and the addition of 15% glucose was omitted. .. Ad cells cultured on glass-bottom dishes (Cellvis, Mountain View, CA, USA) or AR suspended cells mounted on adhesive slide were fixed with 4% paraformaldehyde, permeabilized for 5 minutes with 0.2% Triton X-100, blocked with 10% goat serum for 30 minutes, and then incubated overnight at 4°C with primary antibodies against 5-mC (1:1000; 28692, Cell Signaling Technology, Danvers, MA, USA) for methylation detection, or against CD147 (1:200) and COX IV (a mitochondrial marker; 1:200; 11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147. .. After incubation with fluorescently labeled secondary antibodies, nuclei were counterstained with DAPI (Invitrogen).

Saline:

Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Article Snippet: 14 The EMBO Journal © The Author(s) transfer to PVDF (Fisher Scientific) membrane. .. The blots were incubated in 2% BSA/Tri-buffered saline tween-20 (TBST) overnight at 4 °C with primary antibodies specifically for: HSP90 (Santa Cruz, #sc-13119, 1:5000), SEL1L (home-made, 1:10,000) (Zhou et al, 2020), SEL1L (Abcam, ab78298, 1:1000), HRD1 (Proteintech, #13473-1, 1:2000), CD147 (Proteintech, #11989-1, 1:3000), IRE1α (Cell Signaling, #3294, 1:2000), ERLEC1 (Abcam, #ab181166, 1:5000), BiP/GRP94 (Abcam, #ab21685, 1:5000), PDI (Enzo, #ADI-SPA-890, 1:5000), PERK (Cell Signaling, #3192, 1:5000). .. Membranes were washed with TBST and incubated with secondary antibodies, either HRP conjugated (Bio-Rad, 1:10,000), anti-Rabbit IgG TrueBlot HRP (Rockland, #18-8816-33, 1:500) or anti-Mouse IgG TrueBlot-HRP (Rockland, #18-8817-31, 1:500) at room temperature for 1 h for ECL chemiluminescence detection system (Bio-Rad) development.



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