Journal: Microbiology Spectrum
Article Title: Coxsackievirus A10 blocks autophagosome-lysosome fusion to promote viral nonlytic spread and inflammatory cytokine release
doi: 10.1128/spectrum.00830-25
Figure Lengend Snippet: Inflammatory cytokines and viruses were detected in the extracellular vesicles of CV-A10-infected cells. ( A ) A heatmap presenting the results of inflammatory cytokines in extracellular vesicles by flow cytometry. ( B ) The proliferative dynamics of CV-A10 in extracellular vesicles were assessed by monitoring the viral load, virus titer, and VP1 expression via qRT-PCR, TCID 50 , and WB assays, respectively.
Article Snippet: In this study, we used the commercial Capture Extracellular Vesicle Isolation Kit (Takara, Japan), following the manufacturer’s instructions to obtain extracellular vesicles.
Techniques: Infection, Flow Cytometry, Virus, Expressing, Quantitative RT-PCR