calu1 atcc cat  (ATCC)

Journal: JID Innovations

Article Title: Human keratinocytes exhibit limited potential for SARS-CoV-2 infection despite ACE2 and mature cathepsin L expression

doi: 10.1016/j.xjidi.2025.100447

Figure Lengend Snippet: Quantification of ACE2 , dACE2 , TMPRSS2, NRP1, CTSL , BSG (CD147), DPP4 (CD26), AXL , KREMEN1 , and ASGR1 gene expression. mRNA levels normalized to GAPDH (2 -ΔCT ) in ( a ) NHDF, NHEKs, RHE, and human skin samples and in ( b ) Caco-2/TC-7, Calu-3, and HaCaT cell lines. Gene expression was quantified by RT-qPCR in triplicate. Data represent the mean ± SEM from ( a ) 3 to 14 independent experiments using biological samples from different individuals and ( b ) 3 or 4 independent experiments from cultured cells. NHDF, normal human dermal fibroblast; NHEK, normal human epidermal keratinocyte; RHE, reconstructed human epidermis.

Article Snippet: Human lung adenocarcinoma Calu-3 (Sterlab, Vallauris, France), human colon adenocarcinoma Caco-2/TC-7 (Sigma-Aldrich, St-Quentin-Fallavier, France), human epidermal keratinocyte cell line HaCaT (CLS, Köhln, Germany), and human promonocytic myeloid leukemia cell line U937 (ATCC, Manassas, VA) were maintained in complete MEM, IMDM, or DMEM medium (Gibco-Thermo Fisher Scientific, Courtaboeuf, France) supplemented with 10% fetal calf serum (Pierce, Thermo Fisher Scientific), penicillin (100 IU/ml), and streptomycin (100 μg/ml) (Gibco-Thermo Fisher Scientific) at 37 °C in a humidified atmosphere with 5% carbon dioxide.

Techniques: Gene Expression, Quantitative RT-PCR, Cell Culture

Journal: bioRxiv

Article Title: AVE0991, a Mas receptor agonist, increases influenza and COVID-19 severity in vivo

doi: 10.64898/2026.02.23.707600

Figure Lengend Snippet: Calu-3 cells were infected with MOI 0.1 IAV (A, B) or MOI 0.01 SARS-CoV-2 Delta (C, D) for 24 hours, then treated with vehicle (0.125% DMSO) or AVE0991 (12.5 μM) for 24 h. (A, C) Log 2 fold change in IL-6 and TNF-α gene expression. (B, D) Viral titres in supernatants (PFU/mL) measured by plaque assay. Normality was assessed by Shapiro-Wilk test. Statistical significance was determined using unpaired t-test or Mann-Whitney test, as appropriate. Data represents three independent replicates and are shown as mean ± SEM. *: p<0.05; **: p<0.01; ****: p<0.0001.

Article Snippet: Calu-3 cells (ATCC; HTB-55) were maintained as described in Supplementary Material.

Techniques: Infection, Gene Expression, Plaque Assay, MANN-WHITNEY