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cal120  (DSMZ)


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    Structured Review

    DSMZ cal120
    Cal120, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cal120/CAL-120/pm41803880-73-24-30
    Average 94 stars, based on 57 article reviews
    cal120 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    other:

    Article Title: Control of Breast Cancer Pathogenesis by Histone Methylation and the Hairless Histone Demethylase
    Article Snippet: CAL120 , Ductal carcinoma , Basal-like , DSMZ.

    Article Title: Loss of chromosome cytoband 13q14.2 orchestrates breast cancer pathogenesis and drug response
    Article Snippet: Human BCa cell lines MFM223, CAL120 and EVSA-T were purchased from Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, who provided the authentication certi cate.

    Multiple Displacement Amplification:

    Article Title: Orphan Nuclear Receptors expression and function in breast cancer cells: oncogenic action of the NR2F6 receptor.
    Article Snippet: .. Cell Cultures: MCF7, MDA-MB-231, MDA-MB-453, MDA-MB-436, SKBR3, MDA-MB-157, T47D, CAMA1 and MB-157 cell-lines were from ATCC (AmericanType-Culture-Collection), the Hs578T cell-line was from Sigma-Aldrich, while CAL120 and CAL85.1 cell-lines were from DSMZ (Deutsche-Sammlung-vonMikroorganismen-und-Zellkulturen-GmbH). .. Cells were cultured in phenol-redfree DMEM/F-12 medium (Invitrogen-ThermoFisher-SCIENTIFIC) supplemented with glutamine (2 mM), penicillin (100 units/mL)/streptomycin (100 μg/ml) and 5% fetal-bovine-serum (Euroclone).

    Article Title: Acetyl-CoA synthetase 2 (ACSS2) inhibitors and methods using same
    Article Snippet: .. BT474c1, Brpkp110, A7C11, BT20 (ATCC), SKBr3 (ATCC), HCC1806 (ATCC), MDA-MB-468 (ATCC), MDA-MB-231 (ATCC), HCC1954 (ATCC), MDA-MB-231 (ATCC), Cal120 (DSMZ), Cal51 (DSMZ), MCF7 (ATCC), BT549 (ATCC) cells were cultured in 1×DMEM/F-12 50/50 supplemented (Life Technologies) with 10% fetal bovine serum (FBS) (Life Technologies) and 1× penicillin-streptomycin. .. Hs578t (ATCC) cells were cultured in 1×DMEM/F-12 50/50 supplemented with 0.01 mg/ml bovine insulin (Sigma), 10% FBS, and 1× penicillin-streptomycin.

    Cell Culture:

    Article Title: Acetyl-CoA synthetase 2 (ACSS2) inhibitors and methods using same
    Article Snippet: .. BT474c1, Brpkp110, A7C11, BT20 (ATCC), SKBr3 (ATCC), HCC1806 (ATCC), MDA-MB-468 (ATCC), MDA-MB-231 (ATCC), HCC1954 (ATCC), MDA-MB-231 (ATCC), Cal120 (DSMZ), Cal51 (DSMZ), MCF7 (ATCC), BT549 (ATCC) cells were cultured in 1×DMEM/F-12 50/50 supplemented (Life Technologies) with 10% fetal bovine serum (FBS) (Life Technologies) and 1× penicillin-streptomycin. .. Hs578t (ATCC) cells were cultured in 1×DMEM/F-12 50/50 supplemented with 0.01 mg/ml bovine insulin (Sigma), 10% FBS, and 1× penicillin-streptomycin.



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    Image Search Results


    Overexpression of TBC1D22B, but not of TBC1D22A, induces tumor spheroids overgrowth, in a GAP‐dependent manner. BT549 cells stably expressing the empty vector (EV), HA‐tagged TBC1D22B, RQ or TBC1D22A were grown in matrigel for 7 days to form tumor cell spheroids. Spheroids were stained with Phalloidin and DAPI shown respectively in magenta and grey in the merged representative images (left). Images are confocal Z‐stack projections (8 optical sections/image) of spheroids acquired with a 40x objective, zoom 1.5x. Bar, 50 µm. Quantification of the spheroids area, shown in the middle panel, is from 3 independent experiments in which 45–71 spheroids/condition were measured. Mean ± SD are also shown in the plots. Statistical analysis was performed with the t‐test, **** p < 0.0001. The Western blots on the right were IB with the indicated antibodies (vinculin, loading control) and show the expression levels of TBC1D22B, RQ and TBC1D22A. B) CAL120 cells stably expressing the empty vector (EV), the HA‐tagged TBC1D22B, or RQ were grown and stained as in A. Images are confocal Z‐stack projections (8 optical sections/image) of representative spheroids acquired with a 20x objective, zoom 2. Bar, 50 µm. In the middle panel the area of 45 spheroids/condition from 3 independent experiments is shown. Mean ± SD are also shown in the plots. Statistical analysis was performed with the t‐test, p values: ***, p < 0.001, **** p < 0.0001. The Western blot on the right shows the expression levels of TBC1D22B and RQ.

    Journal: Advanced Science

    Article Title: TBC1D22B Regulates ER‐to‐Golgi Trafficking via RAB1B Inactivation and Promotes Oncogenic Programs in Breast Cancer

    doi: 10.1002/advs.202502269

    Figure Lengend Snippet: Overexpression of TBC1D22B, but not of TBC1D22A, induces tumor spheroids overgrowth, in a GAP‐dependent manner. BT549 cells stably expressing the empty vector (EV), HA‐tagged TBC1D22B, RQ or TBC1D22A were grown in matrigel for 7 days to form tumor cell spheroids. Spheroids were stained with Phalloidin and DAPI shown respectively in magenta and grey in the merged representative images (left). Images are confocal Z‐stack projections (8 optical sections/image) of spheroids acquired with a 40x objective, zoom 1.5x. Bar, 50 µm. Quantification of the spheroids area, shown in the middle panel, is from 3 independent experiments in which 45–71 spheroids/condition were measured. Mean ± SD are also shown in the plots. Statistical analysis was performed with the t‐test, **** p < 0.0001. The Western blots on the right were IB with the indicated antibodies (vinculin, loading control) and show the expression levels of TBC1D22B, RQ and TBC1D22A. B) CAL120 cells stably expressing the empty vector (EV), the HA‐tagged TBC1D22B, or RQ were grown and stained as in A. Images are confocal Z‐stack projections (8 optical sections/image) of representative spheroids acquired with a 20x objective, zoom 2. Bar, 50 µm. In the middle panel the area of 45 spheroids/condition from 3 independent experiments is shown. Mean ± SD are also shown in the plots. Statistical analysis was performed with the t‐test, p values: ***, p < 0.001, **** p < 0.0001. The Western blot on the right shows the expression levels of TBC1D22B and RQ.

    Article Snippet: CAL120 cells, from DSMZ, were grown in DMEM (ECM0749L, Euroclone) supplemented with 10% fetal bovine serum (ECS1800D, Euroclone), 1% L‐glutamine (ECB3000D, Euroclone) and 1% penicillin‐streptomycin (ECB3001D, Euroclone).

    Techniques: Over Expression, Stable Transfection, Expressing, Plasmid Preparation, Staining, Western Blot, Control