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caki 2  (ATCC)


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    ATCC caki 2
    Caki 2, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caki/Caki-2/pm42308695-58-10-19
    Average 96 stars, based on 598 article reviews
    caki 2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Design, synthesis, and biological profiling of fluorinated cannabidiol and cannabigerol derivatives as promising therapeutic agents
    Article Snippet: Graphs were plotted using GraphPad Prism 10.4.1 (627) (GraphPad Software, LLC). .. Human renal carcinoma cell lines, A-498 and CAKI-2 obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% Fetal Bovine Serum (FBS) and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin) and maintained at 37 °C in a humidified atmosphere under 5% CO 2 /95% air. ..

    Article Title: Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumors
    Article Snippet: .. BON-1 cells were cultured in DMEM/F12; STC-1, HT-1080, and Hep G2 were cultured in DMEM; 786-O, QGP-1, and GOT-1 were cultured in ATCC-formulated RPMI-1640; Caki-1 was cultured in McCoy’s 5A. .. All media were supplemented with 10% fetal bovine serum (FBS) (Hyclone, Cytavia) and 1% penicillin/streptomycin (Gibco).

    Article Title: Design, synthesis, and biological profiling of fluorinated cannabidiol and cannabigerol derivatives as promising therapeutic agents.
    Article Snippet: Graphs were plotted using GraphPad Prism 10.4.1 (627) (GraphPad Software, LLC). .. Cell culturing of RCC cell lines Human renal carcinoma cell lines, A-498 and CAKI-2 obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% Fetal Bovine Serum (FBS) and AR TIC LE IN PR ES S antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin) and maintained at 37 °C in a humidified atmosphere under 5% CO2/95% air. ..

    Article Title: Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumors
    Article Snippet: .. BON-1 cells were cultured in DMEM/F12; STC-1, HT-1080, and Hep G2 were cultured in DMEM; 786-O, QGP-1, and GOT-1 were cultured in ATCC-formulated RPMI-1640; Caki-1 was cultured in McCoy’s 5A. .. All media were supplemented with 10% fetal bovine serum (FBS) (Hyclone, Cytavia) and 1% penicillin/streptomycin (Gibco).

    Article Title: Timescale-dependent Phosphoproteomic Remodeling and Motility-associated Adaptation under Chronic Cabozantinib Exposure in Renal Cell Carcinoma
    Article Snippet: .. Parental 786-O and Caki-1 renal cell carcinoma cell lines were obtained from ATCC (Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing 5% CO 2 . ..

    Modification:

    Article Title: Design, synthesis, and biological profiling of fluorinated cannabidiol and cannabigerol derivatives as promising therapeutic agents
    Article Snippet: Graphs were plotted using GraphPad Prism 10.4.1 (627) (GraphPad Software, LLC). .. Human renal carcinoma cell lines, A-498 and CAKI-2 obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% Fetal Bovine Serum (FBS) and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin) and maintained at 37 °C in a humidified atmosphere under 5% CO 2 /95% air. ..

    Article Title: Design, synthesis, and biological profiling of fluorinated cannabidiol and cannabigerol derivatives as promising therapeutic agents.
    Article Snippet: Graphs were plotted using GraphPad Prism 10.4.1 (627) (GraphPad Software, LLC). .. Cell culturing of RCC cell lines Human renal carcinoma cell lines, A-498 and CAKI-2 obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% Fetal Bovine Serum (FBS) and AR TIC LE IN PR ES S antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin) and maintained at 37 °C in a humidified atmosphere under 5% CO2/95% air. ..



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    MUC3A is aberrantly upregulated in ccRCC and associated with poor prognosis. (A) Pan-cancer analysis of MUC3A expression across multiple tumor types based on TCGA data, generated using the GEPIA2 platform. Gene expression values are presented as log2(TPM + 1). (B) Differential expression of MUC3A in tumor and normal samples from the TCGA-KIRC cohort (523 tumor samples vs. 72 normal samples). *P<0.05. (C) Western blotting of MUC3A protein expression in HK-2 and RPTEC/TERT1 non-malignant renal epithelial cell lines and ccRCC cell lines <t>(CAKI-1,</t> OSRC-2, 786-O and ACHN). GAPDH was used as a loading control. (D) Western blotting of MUC3A knockdown efficiency in 786-O and OSRC-2 cells following transient transfection with three independent siRNAs targeting MUC3A. si-2 was selected for subsequent functional experiments due to its superior knockdown efficiency. (E) Kaplan-Meier OS analysis of ccRCC patients stratified into high- and low-MUC3A expression groups using the GEPIA2 platform. (F) Kaplan-Meier DFS analysis of ccRCC patients based on MUC3A expression levels. Data are derived from TCGA unless otherwise indicated. MUC3A, mucin 3A; ccRCC, clear cell renal cell carcinoma; TCGA, The Cancer Genome Atlas; KIRC, kidney renal clear cell carcinoma; GEPIA2, Gene Expression Profiling Interactive Analysis 2; TPM, transcripts per million; KIRC, kidney renal clear cell carcinoma; OS, overall survival; DFS, disease-free survival; siRNA, small interfering RNA; si-MUC3A, MUC3A-targeting siRNA; si-Ctrl, non-targeting control siRNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; si-1/si-2/si-3, three independent siRNAs targeting MUC3A.
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    Image Search Results


    MUC3A is aberrantly upregulated in ccRCC and associated with poor prognosis. (A) Pan-cancer analysis of MUC3A expression across multiple tumor types based on TCGA data, generated using the GEPIA2 platform. Gene expression values are presented as log2(TPM + 1). (B) Differential expression of MUC3A in tumor and normal samples from the TCGA-KIRC cohort (523 tumor samples vs. 72 normal samples). *P<0.05. (C) Western blotting of MUC3A protein expression in HK-2 and RPTEC/TERT1 non-malignant renal epithelial cell lines and ccRCC cell lines (CAKI-1, OSRC-2, 786-O and ACHN). GAPDH was used as a loading control. (D) Western blotting of MUC3A knockdown efficiency in 786-O and OSRC-2 cells following transient transfection with three independent siRNAs targeting MUC3A. si-2 was selected for subsequent functional experiments due to its superior knockdown efficiency. (E) Kaplan-Meier OS analysis of ccRCC patients stratified into high- and low-MUC3A expression groups using the GEPIA2 platform. (F) Kaplan-Meier DFS analysis of ccRCC patients based on MUC3A expression levels. Data are derived from TCGA unless otherwise indicated. MUC3A, mucin 3A; ccRCC, clear cell renal cell carcinoma; TCGA, The Cancer Genome Atlas; KIRC, kidney renal clear cell carcinoma; GEPIA2, Gene Expression Profiling Interactive Analysis 2; TPM, transcripts per million; KIRC, kidney renal clear cell carcinoma; OS, overall survival; DFS, disease-free survival; siRNA, small interfering RNA; si-MUC3A, MUC3A-targeting siRNA; si-Ctrl, non-targeting control siRNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; si-1/si-2/si-3, three independent siRNAs targeting MUC3A.

    Journal: Oncology Reports

    Article Title: Mechanistic study of MUC3A in promoting progression of clear cell renal cell carcinoma via the JAK-STAT pathway

    doi: 10.3892/or.2026.9119

    Figure Lengend Snippet: MUC3A is aberrantly upregulated in ccRCC and associated with poor prognosis. (A) Pan-cancer analysis of MUC3A expression across multiple tumor types based on TCGA data, generated using the GEPIA2 platform. Gene expression values are presented as log2(TPM + 1). (B) Differential expression of MUC3A in tumor and normal samples from the TCGA-KIRC cohort (523 tumor samples vs. 72 normal samples). *P<0.05. (C) Western blotting of MUC3A protein expression in HK-2 and RPTEC/TERT1 non-malignant renal epithelial cell lines and ccRCC cell lines (CAKI-1, OSRC-2, 786-O and ACHN). GAPDH was used as a loading control. (D) Western blotting of MUC3A knockdown efficiency in 786-O and OSRC-2 cells following transient transfection with three independent siRNAs targeting MUC3A. si-2 was selected for subsequent functional experiments due to its superior knockdown efficiency. (E) Kaplan-Meier OS analysis of ccRCC patients stratified into high- and low-MUC3A expression groups using the GEPIA2 platform. (F) Kaplan-Meier DFS analysis of ccRCC patients based on MUC3A expression levels. Data are derived from TCGA unless otherwise indicated. MUC3A, mucin 3A; ccRCC, clear cell renal cell carcinoma; TCGA, The Cancer Genome Atlas; KIRC, kidney renal clear cell carcinoma; GEPIA2, Gene Expression Profiling Interactive Analysis 2; TPM, transcripts per million; KIRC, kidney renal clear cell carcinoma; OS, overall survival; DFS, disease-free survival; siRNA, small interfering RNA; si-MUC3A, MUC3A-targeting siRNA; si-Ctrl, non-targeting control siRNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; si-1/si-2/si-3, three independent siRNAs targeting MUC3A.

    Article Snippet: Human ccRCC cell lines 786-O (cat. no. CL-0010), OSRC-2 (cat. no. CL-0177), Caki-1 (cat. no. CL-0052) and ACHN (cat. no. CL-0021), as well as non-malignant renal epithelial cells HK-2 (cat. no. CL-0109; all from Procell Life Science & Technology Co., Ltd.) and RPTEC/TERT1 (cat. no. CRL-4031; American Type Culture Collection), were used in the present study.

    Techniques: Expressing, Generated, Gene Expression, Quantitative Proteomics, Western Blot, Control, Knockdown, Transfection, Functional Assay, Derivative Assay, Small Interfering RNA

    (A) Six predicted cER genes were investigated in SK-mel-2 cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.

    Journal: PLOS Computational Biology

    Article Title: CASER: A semi-supervised model with multi-omics data integration prioritizes cancer-associated epigenetic regulator genes

    doi: 10.1371/journal.pcbi.1014253

    Figure Lengend Snippet: (A) Six predicted cER genes were investigated in SK-mel-2 cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.

    Article Snippet: The human melanoma cell line SK-mel-2, clear cell renal cell carcinoma cell line Caki-1, breast cancer cell line MDA-MB-231, and prostate cancer cell line LNCaP, along with their corresponding complete median, were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

    Techniques: