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cacng7  (Proteintech)


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    Structured Review

    Proteintech cacng7
    Verification of CAMK2D (CA) and CNN3 (CN) but Not CACNB4 and <t>CACNG7</t> as Cognate Targets of miR-7-5p (A) TargetScan-predicted binding sites of targets with miR-7-5p. (B) Effects of miR-7-5p on the expression levels of CACNB4, CACNG7, CAMK2D, and CNN3. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. (C) Diagrammatic sketch of the binding sites for the WT and MUT binding sites of CAMK2D and CNN3 associated with miR-7-5p. (D) Luciferase reporter assay for the luciferase activity of HPASMCs cotransfected with scrambled miRNA (NC) and VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC) or miR-7-5p mimics with VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC). ∗∗p < 0.01 compared with NC + WT; ##p < 0.01 compared with NC + miR-7-5p-PC. (E and F) HPASMCs were labeled with antibodies against CNN3 (E) and CAMK2D (F), and nuclei were stained with DAPI and merged to represent an overlay figure. Scale bars, 25 μm. All tests were performed at least three times, and the values are presented as the mean ± SEM.
    Cacng7, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacng7/product/Proteintech
    Average 91 stars, based on 1 article reviews
    cacng7 - by Bioz Stars, 2026-02
    91/100 stars

    Images

    1) Product Images from "circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p"

    Article Title: circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p

    Journal: Molecular Therapy. Nucleic Acids

    doi: 10.1016/j.omtn.2020.09.018

    Verification of CAMK2D (CA) and CNN3 (CN) but Not CACNB4 and CACNG7 as Cognate Targets of miR-7-5p (A) TargetScan-predicted binding sites of targets with miR-7-5p. (B) Effects of miR-7-5p on the expression levels of CACNB4, CACNG7, CAMK2D, and CNN3. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. (C) Diagrammatic sketch of the binding sites for the WT and MUT binding sites of CAMK2D and CNN3 associated with miR-7-5p. (D) Luciferase reporter assay for the luciferase activity of HPASMCs cotransfected with scrambled miRNA (NC) and VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC) or miR-7-5p mimics with VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC). ∗∗p < 0.01 compared with NC + WT; ##p < 0.01 compared with NC + miR-7-5p-PC. (E and F) HPASMCs were labeled with antibodies against CNN3 (E) and CAMK2D (F), and nuclei were stained with DAPI and merged to represent an overlay figure. Scale bars, 25 μm. All tests were performed at least three times, and the values are presented as the mean ± SEM.
    Figure Legend Snippet: Verification of CAMK2D (CA) and CNN3 (CN) but Not CACNB4 and CACNG7 as Cognate Targets of miR-7-5p (A) TargetScan-predicted binding sites of targets with miR-7-5p. (B) Effects of miR-7-5p on the expression levels of CACNB4, CACNG7, CAMK2D, and CNN3. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. (C) Diagrammatic sketch of the binding sites for the WT and MUT binding sites of CAMK2D and CNN3 associated with miR-7-5p. (D) Luciferase reporter assay for the luciferase activity of HPASMCs cotransfected with scrambled miRNA (NC) and VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC) or miR-7-5p mimics with VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC). ∗∗p < 0.01 compared with NC + WT; ##p < 0.01 compared with NC + miR-7-5p-PC. (E and F) HPASMCs were labeled with antibodies against CNN3 (E) and CAMK2D (F), and nuclei were stained with DAPI and merged to represent an overlay figure. Scale bars, 25 μm. All tests were performed at least three times, and the values are presented as the mean ± SEM.

    Techniques Used: Binding Assay, Expressing, Luciferase, Reporter Assay, Activity Assay, Labeling, Staining



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    Verification of CAMK2D (CA) and CNN3 (CN) but Not CACNB4 and <t>CACNG7</t> as Cognate Targets of miR-7-5p (A) TargetScan-predicted binding sites of targets with miR-7-5p. (B) Effects of miR-7-5p on the expression levels of CACNB4, CACNG7, CAMK2D, and CNN3. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. (C) Diagrammatic sketch of the binding sites for the WT and MUT binding sites of CAMK2D and CNN3 associated with miR-7-5p. (D) Luciferase reporter assay for the luciferase activity of HPASMCs cotransfected with scrambled miRNA (NC) and VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC) or miR-7-5p mimics with VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC). ∗∗p < 0.01 compared with NC + WT; ##p < 0.01 compared with NC + miR-7-5p-PC. (E and F) HPASMCs were labeled with antibodies against CNN3 (E) and CAMK2D (F), and nuclei were stained with DAPI and merged to represent an overlay figure. Scale bars, 25 μm. All tests were performed at least three times, and the values are presented as the mean ± SEM.
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    Verification of CAMK2D (CA) and CNN3 (CN) but Not CACNB4 and <t>CACNG7</t> as Cognate Targets of miR-7-5p (A) TargetScan-predicted binding sites of targets with miR-7-5p. (B) Effects of miR-7-5p on the expression levels of CACNB4, CACNG7, CAMK2D, and CNN3. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. (C) Diagrammatic sketch of the binding sites for the WT and MUT binding sites of CAMK2D and CNN3 associated with miR-7-5p. (D) Luciferase reporter assay for the luciferase activity of HPASMCs cotransfected with scrambled miRNA (NC) and VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC) or miR-7-5p mimics with VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC). ∗∗p < 0.01 compared with NC + WT; ##p < 0.01 compared with NC + miR-7-5p-PC. (E and F) HPASMCs were labeled with antibodies against CNN3 (E) and CAMK2D (F), and nuclei were stained with DAPI and merged to represent an overlay figure. Scale bars, 25 μm. All tests were performed at least three times, and the values are presented as the mean ± SEM.
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    Image Search Results


    Journal: eLife

    Article Title: Enhanced functional detection of synaptic calcium-permeable AMPA receptors using intracellular NASPM

    doi: 10.7554/eLife.66765

    Figure Lengend Snippet:

    Article Snippet: Transfected construct ( Homo sapiens ) , pIRES-eGFP-γ7 , doi: 10.1038/nn.2266 , , CACNG7 ; OriGene Technologies pCMV6-XL4-γ7 (subcloned into pIRES-eGFP).

    Techniques: Mutagenesis, Transfection, Construct, Software

    Verification of CAMK2D (CA) and CNN3 (CN) but Not CACNB4 and CACNG7 as Cognate Targets of miR-7-5p (A) TargetScan-predicted binding sites of targets with miR-7-5p. (B) Effects of miR-7-5p on the expression levels of CACNB4, CACNG7, CAMK2D, and CNN3. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. (C) Diagrammatic sketch of the binding sites for the WT and MUT binding sites of CAMK2D and CNN3 associated with miR-7-5p. (D) Luciferase reporter assay for the luciferase activity of HPASMCs cotransfected with scrambled miRNA (NC) and VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC) or miR-7-5p mimics with VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC). ∗∗p < 0.01 compared with NC + WT; ##p < 0.01 compared with NC + miR-7-5p-PC. (E and F) HPASMCs were labeled with antibodies against CNN3 (E) and CAMK2D (F), and nuclei were stained with DAPI and merged to represent an overlay figure. Scale bars, 25 μm. All tests were performed at least three times, and the values are presented as the mean ± SEM.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p

    doi: 10.1016/j.omtn.2020.09.018

    Figure Lengend Snippet: Verification of CAMK2D (CA) and CNN3 (CN) but Not CACNB4 and CACNG7 as Cognate Targets of miR-7-5p (A) TargetScan-predicted binding sites of targets with miR-7-5p. (B) Effects of miR-7-5p on the expression levels of CACNB4, CACNG7, CAMK2D, and CNN3. ∗p < 0.05, ∗∗p < 0.01 versus Nor + NC; #p < 0.05, ##p < 0.01 versus Hyp + NC. (C) Diagrammatic sketch of the binding sites for the WT and MUT binding sites of CAMK2D and CNN3 associated with miR-7-5p. (D) Luciferase reporter assay for the luciferase activity of HPASMCs cotransfected with scrambled miRNA (NC) and VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC) or miR-7-5p mimics with VE/CAMK2D and CNN3 3′ UTR-WT/CAMK2D and CNN3 3′ UTR-MUT/complementary sequences of miR-7-5p (miR-7-5p-PC). ∗∗p < 0.01 compared with NC + WT; ##p < 0.01 compared with NC + miR-7-5p-PC. (E and F) HPASMCs were labeled with antibodies against CNN3 (E) and CAMK2D (F), and nuclei were stained with DAPI and merged to represent an overlay figure. Scale bars, 25 μm. All tests were performed at least three times, and the values are presented as the mean ± SEM.

    Article Snippet: The antibodies and reagents used were as follows: Runx2 (ab23981; Abcam, MA, USA); MSX2 (M-70; sc-15396; Santa Cruz Biotechnology, TX, USA); BMP2 (ab14933; Abcam, MA, USA), SOX9 (ab26414; Abcam, MA, USA); SM22α (ab14106; Abcam, MA, USA), CACNB4 (17770-1-AP; Proteintech, IL, USA); CACNG7 (17862-1-AP; Proteintech, IL, USA); CAMK2D (20667-1-AP; Proteintech, IL, USA, and ab52476; Abcam, MA, USA); CNN3 (11509-1-AP; Proteintech, IL, USA); ARS (GMS80046; GENMED, Shanghai, PR China); and the ALP Assay Kit (P0321; Beyotime, Shanghai, PR China).

    Techniques: Binding Assay, Expressing, Luciferase, Reporter Assay, Activity Assay, Labeling, Staining