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rabbit anti caix  (Proteintech)


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    Proteintech rabbit anti caix
    Rabbit Anti Caix, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca9/CA9+Antibody/pm41784166-212-8-10
    Average 95 stars, based on 88 article reviews
    rabbit anti caix - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma
    Article Snippet: The separated protein samples in the gel were then transferred to a polyvinylidene fluoride membrane (Millipore, St Louis, MO). .. Membranes were blocked with 5% skim milk powder (Solarbio, Guangzhou, China) and incubated with primary antibodies, including those against KCNJ2 (1:500; Cat No: 19965-1-AP, Proteintech), HIF1α (1:1000; Cat No: 20960-1-AP, Proteintech), CA9 (1:1000; Cat No: 11071-1-AP, Proteintech), HA (1:1000; Cat No: 66006-2-Ig, Proteintech), and β-actin (1:10,000; Cat No: AC026, ABclonal, Wuhan, China) for 12 h at 4 °C. .. After washing the membrane twice with TBST, it was incubated with horseradish peroxidase-conjugated secondary antibodies, followed by chemiluminescence reagents to detect protein bands (Boster, Wuhan, China). β-Actin expression was used as a loading control.

    Article Title: Effect of isorhamnetin on carbonic anhydrase IX expression and tumorigenesis of bladder cancer by activating PPARγ/PTEN/AKT pathway.
    Article Snippet: Background: To clarify the research prospect and mechanism analysis of isorhamnetin as a therapeutic drug for

    Article Title: KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma.
    Article Snippet: The separated protein samples in the gel were then transferred to a polyvinylidene fluoride membrane (Millipore, St Louis, MO). .. Membranes were blocked with 5% skim milk powder (Solarbio, Guangzhou, China) and incubated with primary antibodies, including those against KCNJ2 (1:500; Cat No: 19965-1-AP, Proteintech), HIF1α (1:1000; Cat No: 20960-1-AP, Proteintech), CA9 (1:1000; Cat No: 11071-1-AP, Proteintech), HA (1:1000; Cat No: 66006-2-Ig, Proteintech), and β-actin (1:10,000; Cat No: AC026, ABclonal, Wuhan, China) for 12 h at 4 °C. .. After washing the membrane twice with TBST, it was incubated with horseradish peroxidase-conjugated secondary antibodies, followed by chemiluminescence reagents to detect protein bands (Boster, Wuhan, China). β-Actin expression was used as a loading control.

    Immunohistochemistry:

    Article Title: Using Multi-Omics Analysis to Explore Diagnostic Tool and Optimize Drug Therapy Selection for Patients with Glioma Based on Cross-Talk Gene Signature
    Article Snippet: .. IHC was performed as described in our previous study [ ], using the following antibodies: anti-POSTN (1:4000; Cat no. 66491-1-Ig, Proteintech, Wuhan, China), anti-CHI3L1 (1:200; Cat no. 12036-1-AP, Proteintech), anti-SAA1 (1:200; Cat no. ab190802, Abcam, USA), anti-MMP9 (1:100; Cat no. 10375-2-AP, Proteintech), CA9 (1:50; Cat no. 11071-1-AP, Proteintech), IL-2 (1:100; Cat no. 26156-1-AP, Proteintech) and IL-6 (1:200; Cat no. 21865-1-AP, Proteintech) antibodies. ..

    Binding Assay:

    Article Title: Effect of isorhamnetin on carbonic anhydrase IX expression and tumorigenesis of bladder cancer by activating PPARγ/PTEN/AKT pathway.
    Article Snippet: Background: To clarify the research prospect and mechanism analysis of isorhamnetin as a therapeutic drug for

    Immunofluorescence:

    Article Title: Targeting PLOD2 induces epithelioid differentiation and improves therapeutic response in sarcomatoid renal cell carcinoma.
    Article Snippet: Images were captured using a biomicroscope (Leica, Germany) and a PANNORAMIC Midi II digital slice scanner (3DHISTECH, Hungary). .. Immunofluorescence (IF) analysis was conducted using antibodies against DCLK1 (1:100, Cell Signaling Technology), ALDH1A1 (1:100, Proteintech), CA9 (1:100, Proteintech), AQP1 (1:100, Proteintech), ZO-1 (1:1000, Proteintech), Snail (1:250, Proteintech) and N-cadherin (1:500, Cell Signaling Technology). ..



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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    Image Search Results


    GA inhibits TNBC growth via targeting CA9. (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9

    doi: 10.3389/fcell.2026.1767397

    Figure Lengend Snippet: GA inhibits TNBC growth via targeting CA9. (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.

    Article Snippet: The CA9 protein (Cusabio, CSB-EP614990HUc0) was coupled to the surface of CM5 chip biosensor (Cytiva, 29104988) in 10 mM sodium acetate (pH 4.5).

    Techniques: Comparison, Expressing, Immunohistochemistry, Derivative Assay, Western Blot, Binding Assay, Quantitative RT-PCR, Control

    Model of the anti-tumor mechanism of GA in TNBC cells. GA could bind CA9 to induce early stage autophagy by blocking PI3K/AKT/mTOR signaling pathway. Moreover, GA suppressed the autophagic flux and increased the p62 to promote ROS production, which activated NLRP3/caspase-1/GSDMD/caspase-3 related pyroptosis and apoptosis to induce TNBC cells death.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9

    doi: 10.3389/fcell.2026.1767397

    Figure Lengend Snippet: Model of the anti-tumor mechanism of GA in TNBC cells. GA could bind CA9 to induce early stage autophagy by blocking PI3K/AKT/mTOR signaling pathway. Moreover, GA suppressed the autophagic flux and increased the p62 to promote ROS production, which activated NLRP3/caspase-1/GSDMD/caspase-3 related pyroptosis and apoptosis to induce TNBC cells death.

    Article Snippet: The CA9 protein (Cusabio, CSB-EP614990HUc0) was coupled to the surface of CM5 chip biosensor (Cytiva, 29104988) in 10 mM sodium acetate (pH 4.5).

    Techniques: Blocking Assay