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JCRB Cell Bank ca9 22
Effects of leflunomide (LEF) on the proliferation and migration of human OSCC cell lines. a Effect of LEF on the growth of human OSCC cell lines SAS, <t>Ca9-22,</t> HSC2, and HSC3. OSCC cells were treated with the indicated amount of LEF or dimethyl sulfoxide (DMSO) as a control for 24, 48, and 72 h, and the CCK-8 assay was performed. b The colony formation assay was also performed to determine the effect of LEF on proliferation into OSCC cells. The left panel shows representative pictures in each cell line, whereas the right panel shows a quantitative analysis of colony formation. Each experiment was performed > 5 times and obtained similar results. Statistical analysis was performed using Student’s t-test. Values are presented as means ± SEM versus control (* p < 0.05)
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Effects of leflunomide (LEF) on the proliferation and migration of human OSCC cell lines. a Effect of LEF on the growth of human OSCC cell lines SAS, Ca9-22, HSC2, and HSC3. OSCC cells were treated with the indicated amount of LEF or dimethyl sulfoxide (DMSO) as a control for 24, 48, and 72 h, and the CCK-8 assay was performed. b The colony formation assay was also performed to determine the effect of LEF on proliferation into OSCC cells. The left panel shows representative pictures in each cell line, whereas the right panel shows a quantitative analysis of colony formation. Each experiment was performed > 5 times and obtained similar results. Statistical analysis was performed using Student’s t-test. Values are presented as means ± SEM versus control (* p < 0.05)

Journal: Discover Oncology

Article Title: Anticancer effect of the antirheumatic drug leflunomide on oral squamous cell carcinoma by the inhibition of tumor angiogenesis

doi: 10.1007/s12672-025-01763-5

Figure Lengend Snippet: Effects of leflunomide (LEF) on the proliferation and migration of human OSCC cell lines. a Effect of LEF on the growth of human OSCC cell lines SAS, Ca9-22, HSC2, and HSC3. OSCC cells were treated with the indicated amount of LEF or dimethyl sulfoxide (DMSO) as a control for 24, 48, and 72 h, and the CCK-8 assay was performed. b The colony formation assay was also performed to determine the effect of LEF on proliferation into OSCC cells. The left panel shows representative pictures in each cell line, whereas the right panel shows a quantitative analysis of colony formation. Each experiment was performed > 5 times and obtained similar results. Statistical analysis was performed using Student’s t-test. Values are presented as means ± SEM versus control (* p < 0.05)

Article Snippet: Human OSCC (hOSCC) cell lines SAS (JCRB0260), Ca9-22 (JCRB0625), HSC2 (JCRB0622), and HSC3 (JCRB0623) were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan).

Techniques: Migration, Control, CCK-8 Assay, Colony Assay