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c4d  (Quidel)


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    Structured Review

    Quidel c4d
    C4d, supplied by Quidel, used in various techniques. Bioz Stars score: 96/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c4d/C4d+Antibody/pm41915761-100-5-9
    Average 96 stars, based on 183 article reviews
    c4d - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Complement activation profile in adult primary immune thrombocytopenia.
    Article Snippet: FITC-conjugated IgG was obtained from Exbio (Prague, Czech Republic). .. Antibodies against C1q, C3d, and C4d were acquired from QuidelOrtho (San Diego, CA) and subsequently labeled with the Alexa Fluor 488 Antibody Labeling Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions. .. Additional reagents used in this assay included prostaglandin E1 from Sigma-Aldrich (St. Louis, MO), Quantum Simply Cellular anti-mouse IgG from Bang Laboratories (Fisher, IN), and normal saline from Otsuka Pharmaceutical (Tokyo, Japan).

    Antibody Labeling:

    Article Title: Complement activation profile in adult primary immune thrombocytopenia.
    Article Snippet: FITC-conjugated IgG was obtained from Exbio (Prague, Czech Republic). .. Antibodies against C1q, C3d, and C4d were acquired from QuidelOrtho (San Diego, CA) and subsequently labeled with the Alexa Fluor 488 Antibody Labeling Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions. .. Additional reagents used in this assay included prostaglandin E1 from Sigma-Aldrich (St. Louis, MO), Quantum Simply Cellular anti-mouse IgG from Bang Laboratories (Fisher, IN), and normal saline from Otsuka Pharmaceutical (Tokyo, Japan).

    Staining:

    Article Title: Engineering human pluripotent stem cell lines to evade xenogeneic transplantation barriers
    Article Snippet: Cells were resuspended in 10% C7-deficient serum (Sigma-Aldrich, cat. no. C1413-1ML) in the appropriate GVB buffer and incubated at 37oC for 45min with shaking. .. Cells were washed with 1% FBS/PBS then stained with mouse anti-human C3c, C3d, C4c, C4d, or C5 antibody (Quidel, cat. no. A205, A207, A211, A213, and A217) for 30min at 4oC. .. Cells were washed with 1% FBS/PBS then stained with PE/Cy7-conjugated rat anti-mouse Ig light chain antibody (BD) for 30min at 4oC.

    Article Title: Engineering human pluripotent stem cell lines to evade xenogeneic transplantation barriers
    Article Snippet: Cells were resuspended in 10% C7-deficient serum (Sigma-Aldrich, cat. no. C1413-1ML) in the appropriate GVB buffer and incubated at 37oC for 45min with shaking. .. Cells were washed with 1% FBS/PBS then stained with mouse anti-human C3c, C3d, C4c, C4d, or C5 antibody (Quidel, cat. no. A205, A207, A211, A213, and A217) for 30min at 4oC. .. Cells were washed with 1% FBS/PBS then stained with PE/Cy7-conjugated rat anti-mouse Ig light chain antibody (BD) for 30min at 4oC.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Early Activation of the Complement System After Brain Death in Clinical Kidney Donation and Transplantation
    Article Snippet: .. The following assays were performed according to the manufacturer’s instructions: C4d (MicroVue C4d EIA, A008; Quidel, San Diego, CA), Bb (MicroVue Bb Plus EIA, A027; Quidel), and C3c (HK368; Hycult Biotech, Uden, the Netherlands). ..

    Article Title: Circulating mortalin in blood and activation of the alternative complement pathway as risk indicators in COVID-19 infection
    Article Snippet: .. The concentrations of properdin (HK334, Hycult Biotech), C1s/C1-INH complex (HK399, Hycult Biotech), MASP-1/C1-INH complex (HK3001, Hycult Biotech), C4a (A035, Quidel), C4d (A009, Quidel), and Bb (A027, Quidel) were determined in blood plasma-EDTA samples by using commercially available ELISA kits. ..

    Article Title: Early Activation of the Complement System After Brain Death in Clinical Kidney Donation and Transplantation
    Article Snippet: .. The following assays were performed according to the manufacturer’s instructions: C4d (MicroVue C4d EIA, A008; Quidel, San Diego, CA), Bb (MicroVue Bb Plus EIA, A027; Quidel), and C3c (HK368; Hycult Biotech, Uden, the Netherlands). ..

    Article Title: Association between urinary C4d levels and disease progression in IgA nephropathy.
    Article Snippet: Background.. C4d mesangial deposition, a hallmark of lectin pathway activation in immunoglobulin A nephropathy (IgAN), has been shown to be associated with risk of kidney failure.. To date, the relationship between urinary C4d and renal outcome remain unelucidated.

    Clinical Proteomics:

    Article Title: Circulating mortalin in blood and activation of the alternative complement pathway as risk indicators in COVID-19 infection
    Article Snippet: .. The concentrations of properdin (HK334, Hycult Biotech), C1s/C1-INH complex (HK399, Hycult Biotech), MASP-1/C1-INH complex (HK3001, Hycult Biotech), C4a (A035, Quidel), C4d (A009, Quidel), and Bb (A027, Quidel) were determined in blood plasma-EDTA samples by using commercially available ELISA kits. ..

    Sandwich ELISA:

    Article Title: 223 Beta cell-derived C3 is protective in a mouse model of diet-induced obesity and diabetes
    Article Snippet: .. C4a, Bb, C3a, C4d, C5a, and SC5b-9 complement factors were measured by sandwich ELISA kits (Quidel). ..



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    Image Search Results


    (A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and C4d (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.

    Journal: bioRxiv

    Article Title: Single-cell atlas of pig-to-monkey kidney xenotransplantation reveals macrophage chimerism and an IFN-ε orchestrated graft protective immune niche

    doi: 10.64898/2026.03.21.713416

    Figure Lengend Snippet: (A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and C4d (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.

    Article Snippet: The primary antibody panel including: anti-IgM (11016-1-AP, 1:100, Proteintech), anti-IgG (ab109489, 1:500, Abcam), C4d (22233-1-AP, 1:200, Proteintech), anti-CD31 (ab28364, 1:50, Abcam), anti-vWF (ab6994, 1:200, Abcam), anti-CD3 (GB12014, 1:1000, Servicebio) and anti-CD15 (YT0726, 1:200, Immunoway) for IHC; anti-CD68 (bs-0649R, 1:200, Bioss), anti-SPP1 (83341-1-RR, 1:200, Proteintech), anti-VEGFA (66828-1-Ig, 1:200, Proteintech), anti-PD-L1 (ab205921, 1:200, Abcam), anti-IDO1 (ab211017, 1:1000, Abcam), anti-ARG1 (66129-1-Ig, 1:200, Proteintech), anti-FCGR2B (NB100-65338, 1:100, Novus Biologicals), anti-CD8a (66868-1-Ig, 1:400, Proteintech), and anti-KLRD1 (84466-5-RR, 1:200, Proteintech) for multiplexed IF.

    Techniques: Control, Immunohistochemistry, Immunofluorescence, Staining