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Structured Review

Proteintech c4d
(A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and <t>C4d</t> (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.
C4d, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Single-cell atlas of pig-to-monkey kidney xenotransplantation reveals macrophage chimerism and an IFN-ε orchestrated graft protective immune niche"

Article Title: Single-cell atlas of pig-to-monkey kidney xenotransplantation reveals macrophage chimerism and an IFN-ε orchestrated graft protective immune niche

Journal: bioRxiv

doi: 10.64898/2026.03.21.713416

(A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and C4d (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.
Figure Legend Snippet: (A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and C4d (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.

Techniques Used: Control, Immunohistochemistry, Immunofluorescence, Staining

Related Articles

Immunohistochemistry:

Article Title: Single-cell atlas of pig-to-monkey kidney xenotransplantation reveals macrophage chimerism and an IFN-ε orchestrated graft protective immune niche
Article Snippet: .. The primary antibody panel including: anti-IgM (11016-1-AP, 1:100, Proteintech), anti-IgG (ab109489, 1:500, Abcam), C4d (22233-1-AP, 1:200, Proteintech), anti-CD31 (ab28364, 1:50, Abcam), anti-vWF (ab6994, 1:200, Abcam), anti-CD3 (GB12014, 1:1000, Servicebio) and anti-CD15 (YT0726, 1:200, Immunoway) for IHC; anti-CD68 (bs-0649R, 1:200, Bioss), anti-SPP1 (83341-1-RR, 1:200, Proteintech), anti-VEGFA (66828-1-Ig, 1:200, Proteintech), anti-PD-L1 (ab205921, 1:200, Abcam), anti-IDO1 (ab211017, 1:1000, Abcam), anti-ARG1 (66129-1-Ig, 1:200, Proteintech), anti-FCGR2B (NB100-65338, 1:100, Novus Biologicals), anti-CD8a (66868-1-Ig, 1:400, Proteintech), and anti-KLRD1 (84466-5-RR, 1:200, Proteintech) for multiplexed IF. ..



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Image Search Results


(A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and C4d (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.

Journal: bioRxiv

Article Title: Single-cell atlas of pig-to-monkey kidney xenotransplantation reveals macrophage chimerism and an IFN-ε orchestrated graft protective immune niche

doi: 10.64898/2026.03.21.713416

Figure Lengend Snippet: (A) Haematoxylin and eosin-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of glomeruli (row 1), peritubular capillaries (row 2), and tubulointerstitial compartments (row 3) are shown. Black arrows show non-infiltrated peritubular capillaries (sections a2 and c2), and infiltrated peritubular capillaries (section b2 and d2). (B) Immunohistochemistry-based evaluation for xenotransplantation control #1 (sections a1-a3) and #2 (sections c1-c3), xenograft #1 (sections b1-b3) and #2 (d1-d3). Fields representative of IgM (row 1), IgG (row 2) and C4d (row 3) stainings are shown. (C) Immunofluorescence staining of infiltrated macrophages with signature proteins. Scale bars, 100 µm.

Article Snippet: The primary antibody panel including: anti-IgM (11016-1-AP, 1:100, Proteintech), anti-IgG (ab109489, 1:500, Abcam), C4d (22233-1-AP, 1:200, Proteintech), anti-CD31 (ab28364, 1:50, Abcam), anti-vWF (ab6994, 1:200, Abcam), anti-CD3 (GB12014, 1:1000, Servicebio) and anti-CD15 (YT0726, 1:200, Immunoway) for IHC; anti-CD68 (bs-0649R, 1:200, Bioss), anti-SPP1 (83341-1-RR, 1:200, Proteintech), anti-VEGFA (66828-1-Ig, 1:200, Proteintech), anti-PD-L1 (ab205921, 1:200, Abcam), anti-IDO1 (ab211017, 1:1000, Abcam), anti-ARG1 (66129-1-Ig, 1:200, Proteintech), anti-FCGR2B (NB100-65338, 1:100, Novus Biologicals), anti-CD8a (66868-1-Ig, 1:400, Proteintech), and anti-KLRD1 (84466-5-RR, 1:200, Proteintech) for multiplexed IF.

Techniques: Control, Immunohistochemistry, Immunofluorescence, Staining