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c42b (ATCC)

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ATCC c42b

SCD-high PCa cell lines show better response to SCD inhibition in 3D culture. (A) Taqman RT-PCR analysis of baseline SCD gene expression in SCD-high (ARCaP(M), 22Rv1) and SCD-low (PC3, <t>C42B)</t> cells. Relative expression (2 −ΔΔct ) is shown as fold-change relative to ARCaP(M). (B) Western blot analyses of SCD expression in ARCaP(M), 22Rv1, PC3, and C42B cells grown alone or in Transwell (TW) co-culture with adipocytes. (C) Densitometric analysis of SCD levels. SCD expression in each cell line was normalized to Tubulin and shown as fold change relative to ARCaP(M) cells grown alone. (D–K) Live/Dead assay on ARCaP(M) (D, E), PC3 (F, G), 22Rv1 (H, I), and C42B (J, K) grown in 3-dimensional (3D) cultures on reconstituted basement membrane (rBM) with a 2% Cultrex overlay. 3D cultures were either grown alone (top panels) or with adipocytes (bottom panels) and treated with vehicle control (0.1% DMSO), siRNA-targeting SCD, or 1 μM CAY10566. Live cells (green fluorescence; calcein AM); dead cells (red fluorescence; ethidium homodimer). Quantification of live (green) spheroid volume compared to control for ARCaP(M) (E), PC3 (G), 22Rv1 (I), and C42B (K) cells grown alone (top panel) or with adipocytes (bottom panel). Data are representative of three experiments. Statistical analysis was performed using Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, and ns = not significant. Error bars represent standard deviation (SD) of the mean. Scale bar: 1 unit = 23.41 μM.

C42b, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3053 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c42b/product/ATCC
Average 99 stars, based on 3053 article reviews

c42b - by Bioz Stars, 2026-03

99/100 stars

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1) Product Images from "Inhibiting stearoyl-CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response"

Article Title: Inhibiting stearoyl-CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

Journal: FEBS letters

doi: 10.1002/1873-3468.70290

Figure Legend Snippet: SCD-high PCa cell lines show better response to SCD inhibition in 3D culture. (A) Taqman RT-PCR analysis of baseline SCD gene expression in SCD-high (ARCaP(M), 22Rv1) and SCD-low (PC3, C42B) cells. Relative expression (2 −ΔΔct ) is shown as fold-change relative to ARCaP(M). (B) Western blot analyses of SCD expression in ARCaP(M), 22Rv1, PC3, and C42B cells grown alone or in Transwell (TW) co-culture with adipocytes. (C) Densitometric analysis of SCD levels. SCD expression in each cell line was normalized to Tubulin and shown as fold change relative to ARCaP(M) cells grown alone. (D–K) Live/Dead assay on ARCaP(M) (D, E), PC3 (F, G), 22Rv1 (H, I), and C42B (J, K) grown in 3-dimensional (3D) cultures on reconstituted basement membrane (rBM) with a 2% Cultrex overlay. 3D cultures were either grown alone (top panels) or with adipocytes (bottom panels) and treated with vehicle control (0.1% DMSO), siRNA-targeting SCD, or 1 μM CAY10566. Live cells (green fluorescence; calcein AM); dead cells (red fluorescence; ethidium homodimer). Quantification of live (green) spheroid volume compared to control for ARCaP(M) (E), PC3 (G), 22Rv1 (I), and C42B (K) cells grown alone (top panel) or with adipocytes (bottom panel). Data are representative of three experiments. Statistical analysis was performed using Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, and ns = not significant. Error bars represent standard deviation (SD) of the mean. Scale bar: 1 unit = 23.41 μM.

Techniques Used: Inhibition, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Expressing, Western Blot, Co-Culture Assay, Live Dead Assay, Membrane, Control, Fluorescence, Standard Deviation

SCD inhibition induces DNA damage in ARCaP(M) and 22Rv1 cells but does not significantly affect DNA damage response in PC3 and C42B cells. (A–D) Immunofluorescence staining (green) of γ-H2A histone family member 1 (H2AX) in ARCaP(M) (A), 22Rv1 (B), PC3 (C) and C42B (D) cells grown alone or in Transwell (TW) coculture with adipocytes and treated with vehicle control (0.1% DMSO) or 1 μM CAY10566 for 48 h; 63× images; Hoechst dye (blue) was used to stain the nuclei. Quantification of γ-H2AX fluorescence in ARCaP(M) (A), 22Rv1 (B), PC3 (C) and C42B (D) cells; (E) ARCaP(M) cells grown alone or in TW co-culture were treated with vehicle control (0.1% DMSO) or 1 μM CAY10566 and subjected to modified alkaline comet assay and stained with SYBR gold; 20× images. (F) DNA damage was quantified for all conditions using the olive moment for ~ 50–100 cells for each condition in the OpenComet plug-in for Fiji 2. Data were log-transformed to normalize the distribution. Data are representative of three experiments, Statistical analysis was performed using Student’s t -test; * P < 0.05; ** P < 0.01; **** P < 0.0001, and ns = not significant. Error bars represent standard deviation (SD) of the mean. Scale bar: A–D = 10 μM; E = 50 μM.

Figure Legend Snippet: SCD inhibition induces DNA damage in ARCaP(M) and 22Rv1 cells but does not significantly affect DNA damage response in PC3 and C42B cells. (A–D) Immunofluorescence staining (green) of γ-H2A histone family member 1 (H2AX) in ARCaP(M) (A), 22Rv1 (B), PC3 (C) and C42B (D) cells grown alone or in Transwell (TW) coculture with adipocytes and treated with vehicle control (0.1% DMSO) or 1 μM CAY10566 for 48 h; 63× images; Hoechst dye (blue) was used to stain the nuclei. Quantification of γ-H2AX fluorescence in ARCaP(M) (A), 22Rv1 (B), PC3 (C) and C42B (D) cells; (E) ARCaP(M) cells grown alone or in TW co-culture were treated with vehicle control (0.1% DMSO) or 1 μM CAY10566 and subjected to modified alkaline comet assay and stained with SYBR gold; 20× images. (F) DNA damage was quantified for all conditions using the olive moment for ~ 50–100 cells for each condition in the OpenComet plug-in for Fiji 2. Data were log-transformed to normalize the distribution. Data are representative of three experiments, Statistical analysis was performed using Student’s t -test; * P < 0.05; ** P < 0.01; **** P < 0.0001, and ns = not significant. Error bars represent standard deviation (SD) of the mean. Scale bar: A–D = 10 μM; E = 50 μM.

Techniques Used: Inhibition, Immunofluorescence, Staining, Control, Fluorescence, Co-Culture Assay, Modification, Alkaline Single Cell Gel Electrophoresis, Transformation Assay, Standard Deviation

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Journal: FEBS letters

Article Title: Inhibiting stearoyl-CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

doi: 10.1002/1873-3468.70290

Figure Lengend Snippet: SCD-high PCa cell lines show better response to SCD inhibition in 3D culture. (A) Taqman RT-PCR analysis of baseline SCD gene expression in SCD-high (ARCaP(M), 22Rv1) and SCD-low (PC3, C42B) cells. Relative expression (2 −ΔΔct ) is shown as fold-change relative to ARCaP(M). (B) Western blot analyses of SCD expression in ARCaP(M), 22Rv1, PC3, and C42B cells grown alone or in Transwell (TW) co-culture with adipocytes. (C) Densitometric analysis of SCD levels. SCD expression in each cell line was normalized to Tubulin and shown as fold change relative to ARCaP(M) cells grown alone. (D–K) Live/Dead assay on ARCaP(M) (D, E), PC3 (F, G), 22Rv1 (H, I), and C42B (J, K) grown in 3-dimensional (3D) cultures on reconstituted basement membrane (rBM) with a 2% Cultrex overlay. 3D cultures were either grown alone (top panels) or with adipocytes (bottom panels) and treated with vehicle control (0.1% DMSO), siRNA-targeting SCD, or 1 μM CAY10566. Live cells (green fluorescence; calcein AM); dead cells (red fluorescence; ethidium homodimer). Quantification of live (green) spheroid volume compared to control for ARCaP(M) (E), PC3 (G), 22Rv1 (I), and C42B (K) cells grown alone (top panel) or with adipocytes (bottom panel). Data are representative of three experiments. Statistical analysis was performed using Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, and ns = not significant. Error bars represent standard deviation (SD) of the mean. Scale bar: 1 unit = 23.41 μM.

Article Snippet: PC3 (RRID:CVCL_0035), 22Rv1 (RRID:CVCL_1045), and C42B (RRID:CVCL_4784) cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Inhibition, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Expressing, Western Blot, Co-Culture Assay, Live Dead Assay, Membrane, Control, Fluorescence, Standard Deviation

Journal: FEBS letters

Article Title: Inhibiting stearoyl-CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

doi: 10.1002/1873-3468.70290

Figure Lengend Snippet: SCD inhibition induces DNA damage in ARCaP(M) and 22Rv1 cells but does not significantly affect DNA damage response in PC3 and C42B cells. (A–D) Immunofluorescence staining (green) of γ-H2A histone family member 1 (H2AX) in ARCaP(M) (A), 22Rv1 (B), PC3 (C) and C42B (D) cells grown alone or in Transwell (TW) coculture with adipocytes and treated with vehicle control (0.1% DMSO) or 1 μM CAY10566 for 48 h; 63× images; Hoechst dye (blue) was used to stain the nuclei. Quantification of γ-H2AX fluorescence in ARCaP(M) (A), 22Rv1 (B), PC3 (C) and C42B (D) cells; (E) ARCaP(M) cells grown alone or in TW co-culture were treated with vehicle control (0.1% DMSO) or 1 μM CAY10566 and subjected to modified alkaline comet assay and stained with SYBR gold; 20× images. (F) DNA damage was quantified for all conditions using the olive moment for ~ 50–100 cells for each condition in the OpenComet plug-in for Fiji 2. Data were log-transformed to normalize the distribution. Data are representative of three experiments, Statistical analysis was performed using Student’s t -test; * P < 0.05; ** P < 0.01; **** P < 0.0001, and ns = not significant. Error bars represent standard deviation (SD) of the mean. Scale bar: A–D = 10 μM; E = 50 μM.

Article Snippet: PC3 (RRID:CVCL_0035), 22Rv1 (RRID:CVCL_1045), and C42B (RRID:CVCL_4784) cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Inhibition, Immunofluorescence, Staining, Control, Fluorescence, Co-Culture Assay, Modification, Alkaline Single Cell Gel Electrophoresis, Transformation Assay, Standard Deviation

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1) Product Images from "Inhibiting stearoyl-CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response"

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