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complement c3/c3a, mouse  (MedChemExpress)


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    Structured Review

    MedChemExpress complement c3/c3a, mouse
    Complement C3/C3a, Mouse, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c3a/Complement+C3%2FC3a%2C+Mouse/custom%40hy-p7863%4042464680
    Average 94 stars, based on 4 article reviews
    complement c3/c3a, mouse - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: An iguratimod prodrug reduces RA-FLS invasiveness by disrupting STAT1-C3-TNFα-mediated crosstalk between fibroblast-like synoviocytes and macrophages.
    Article Snippet: For differentiation into macrophage-like cells, THP-1 cells were treated with 100 ng/mL phorbol 12-myristate 13-acetate (PMA; MCE, HY-18739) for 48 h, followed by replacement with fresh complete medium without PMA and further incubation for 24 h. After treatment, the cells exhibited adherent growth and macrophage-like morphology. .. The differentiated cells were cultured for 24 h under three conditions: untreated, 100 nM C3a (R&D Systems, 3677-C3), or 100 nM C3a plus 100 nM JR14a (MCE, HY-138161). .. Wistar rats (8 weeks, 200 ± 20 g, SPF-grade; Beijing HFK Bioscience) were housed under controlled conditions (20–26 ◦C, 40–70% humidity, <60 dB, 12 h light/dark cycle) with free access to food and water.

    In Vitro:

    Article Title: Inhibition of C5AR1 impairs osteoclast mobilization and prevents bone loss.
    Article Snippet: Received 12 July 2022; accepted 28 April 2023; https://doi.org/10.1016/j.ymthe.2023.04.022.. Correspondence: Francesc Ventura, Departament de Ciències Fisiològiques, Universitat de Barcelona, IDIBELL, C/ Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Spain.. E-mail: fventura@ub.edu Age-related and chemotherapy-induced bone loss depends on cellular senescence and the cell secretory phenotype.

    Concentration Assay:

    Article Title: Activation of the C3a-C3aReceptor-axis is associated with endothelial dysfunction and glycocalyx damage in ST-elevation myocardial infarction.
    Article Snippet: .. Further, HUVEC and HCMEC were stimulated with 250 ng/mL C3a with or without the (i) C3a receptor antagonist (C3aRA) SB290157 (Sigma Aldrich, Hamburg, Germany; catalog: 559410) [concentration 1 μg/mL (1:1000)], (ii) C3aRA JR14a (MedChemExpress, NJ, USA; catalog: HY-138161) [concentration 100nM], (iii) C5a Receptor-1 antagonist (C5aRA) PMX53 (Sigma Aldrich, Hamburg, Germany; catalog: 533683) [concentration 1 μg/mL (1:1000)], (iv) Rac1-GTPase-Inhibitor (Rac-I) NSC23766 (Sigma Aldrich, Hamburg, Germany; catalog: SML0952) [concentration: 50 μM] for 24 h. Cells were treated with the Rac-I to assess the functional necessity of Rac1 activity downstream of C3a/ C3aR signaling. ..

    Functional Assay:

    Article Title: Activation of the C3a-C3aReceptor-axis is associated with endothelial dysfunction and glycocalyx damage in ST-elevation myocardial infarction.
    Article Snippet: .. Further, HUVEC and HCMEC were stimulated with 250 ng/mL C3a with or without the (i) C3a receptor antagonist (C3aRA) SB290157 (Sigma Aldrich, Hamburg, Germany; catalog: 559410) [concentration 1 μg/mL (1:1000)], (ii) C3aRA JR14a (MedChemExpress, NJ, USA; catalog: HY-138161) [concentration 100nM], (iii) C5a Receptor-1 antagonist (C5aRA) PMX53 (Sigma Aldrich, Hamburg, Germany; catalog: 533683) [concentration 1 μg/mL (1:1000)], (iv) Rac1-GTPase-Inhibitor (Rac-I) NSC23766 (Sigma Aldrich, Hamburg, Germany; catalog: SML0952) [concentration: 50 μM] for 24 h. Cells were treated with the Rac-I to assess the functional necessity of Rac1 activity downstream of C3a/ C3aR signaling. ..

    Activity Assay:

    Article Title: Activation of the C3a-C3aReceptor-axis is associated with endothelial dysfunction and glycocalyx damage in ST-elevation myocardial infarction.
    Article Snippet: .. Further, HUVEC and HCMEC were stimulated with 250 ng/mL C3a with or without the (i) C3a receptor antagonist (C3aRA) SB290157 (Sigma Aldrich, Hamburg, Germany; catalog: 559410) [concentration 1 μg/mL (1:1000)], (ii) C3aRA JR14a (MedChemExpress, NJ, USA; catalog: HY-138161) [concentration 100nM], (iii) C5a Receptor-1 antagonist (C5aRA) PMX53 (Sigma Aldrich, Hamburg, Germany; catalog: 533683) [concentration 1 μg/mL (1:1000)], (iv) Rac1-GTPase-Inhibitor (Rac-I) NSC23766 (Sigma Aldrich, Hamburg, Germany; catalog: SML0952) [concentration: 50 μM] for 24 h. Cells were treated with the Rac-I to assess the functional necessity of Rac1 activity downstream of C3a/ C3aR signaling. ..

    Incubation:

    Article Title: Age-Related Complement C3 Drives Memory Impairments and Associated Neuropathologies in a Mouse Model.
    Article Snippet: .. The plates were incubated at 37°C with 5% CO2 for 24 h. The medium was then replaced with fresh medium containing either 200 nM C3a (HY- P7862, MCE, USA), a C3aR competitive antagonists (SB290157, HY- 101502A, MCE), or the respective control medium. ..

    Article Title: Age‐Related Complement C3 Drives Memory Impairments and Associated Neuropathologies in a Mouse Model
    Article Snippet: .. The plates were incubated at 37°C with 5% CO 2 for 24 h. The medium was then replaced with fresh medium containing either 200 nM C3a (HY‐P7862, MCE, USA), a C3aR competitive antagonists (SB290157, HY‐101502A, MCE), or the respective control medium. ..

    Control:

    Article Title: Age-Related Complement C3 Drives Memory Impairments and Associated Neuropathologies in a Mouse Model.
    Article Snippet: .. The plates were incubated at 37°C with 5% CO2 for 24 h. The medium was then replaced with fresh medium containing either 200 nM C3a (HY- P7862, MCE, USA), a C3aR competitive antagonists (SB290157, HY- 101502A, MCE), or the respective control medium. ..

    Article Title: Age‐Related Complement C3 Drives Memory Impairments and Associated Neuropathologies in a Mouse Model
    Article Snippet: .. The plates were incubated at 37°C with 5% CO 2 for 24 h. The medium was then replaced with fresh medium containing either 200 nM C3a (HY‐P7862, MCE, USA), a C3aR competitive antagonists (SB290157, HY‐101502A, MCE), or the respective control medium. ..



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    ATCC hepatocellular carcinoma hepg2 c3a cell line
    (A) Global histone succinylation measured by LC-MS/MS <t>in</t> <t>HepG2/C3A</t> cells following 48 hours of sodium succinate treatment. (B) Global histone succinylation in 3D cultured HepG2/C3A spheroids following 48 hours and 1 week of sodium succinate treatment. (C) Comparison of the relative abundance of succinyl-lysine residues in H3 and H4, in either treated or untreated, cultured cells (2D) or spheroids (3D). (D) Structure of the nucleosome with H3K64 residues highlighted to demonstrate solvent accessibility. (E) Abundance of succinyl-CoA in the nuclear fraction (left) and non-nuclear fraction (right) following sodium succinate treatment. (F) Total count of HepG2/C3A cells treated with 10 mM sodium succinate continuously for 48 to 144 hours, cell count taken every 48 hours. Data are shown as mean ± SEM; * p-value <0 . 05 in two-tailed Student’s t-test .
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    Image Search Results


    (A) Global histone succinylation measured by LC-MS/MS in HepG2/C3A cells following 48 hours of sodium succinate treatment. (B) Global histone succinylation in 3D cultured HepG2/C3A spheroids following 48 hours and 1 week of sodium succinate treatment. (C) Comparison of the relative abundance of succinyl-lysine residues in H3 and H4, in either treated or untreated, cultured cells (2D) or spheroids (3D). (D) Structure of the nucleosome with H3K64 residues highlighted to demonstrate solvent accessibility. (E) Abundance of succinyl-CoA in the nuclear fraction (left) and non-nuclear fraction (right) following sodium succinate treatment. (F) Total count of HepG2/C3A cells treated with 10 mM sodium succinate continuously for 48 to 144 hours, cell count taken every 48 hours. Data are shown as mean ± SEM; * p-value <0 . 05 in two-tailed Student’s t-test .

    Journal: bioRxiv

    Article Title: Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states

    doi: 10.64898/2026.05.29.728167

    Figure Lengend Snippet: (A) Global histone succinylation measured by LC-MS/MS in HepG2/C3A cells following 48 hours of sodium succinate treatment. (B) Global histone succinylation in 3D cultured HepG2/C3A spheroids following 48 hours and 1 week of sodium succinate treatment. (C) Comparison of the relative abundance of succinyl-lysine residues in H3 and H4, in either treated or untreated, cultured cells (2D) or spheroids (3D). (D) Structure of the nucleosome with H3K64 residues highlighted to demonstrate solvent accessibility. (E) Abundance of succinyl-CoA in the nuclear fraction (left) and non-nuclear fraction (right) following sodium succinate treatment. (F) Total count of HepG2/C3A cells treated with 10 mM sodium succinate continuously for 48 to 144 hours, cell count taken every 48 hours. Data are shown as mean ± SEM; * p-value <0 . 05 in two-tailed Student’s t-test .

    Article Snippet: The human hepatocellular carcinoma HepG2/C3A cell line was obtained from the American Type Culture Collection (ATCC, CRL-10741).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Cell Culture, Comparison, Solvent, Cell Characterization, Two Tailed Test

    (A) Schematic of peptide pull-down technique used to identify potential protein interactors with histone succinylation. (B) Volcano plot of fold change and significance of enrichment of nuclear proteins from HepG2/C3A cells to a Ksu synthetic peptide vs. Kac synthetic peptide. (C) Volcano plot of fold change and significance of enrichment of bromodomain proteins to Ksu synthetic histone peptide as compared to the Kac peptide, and (D) Ksu synthetic histone peptide as compared to an unmodified peptide. (E) Volcano plot of fold change and significance of enrichment of JmjC domain demethylases to Ksu synthetic histone peptide as compared to an Kac peptide and (F) Ksu synthetic histone peptide as compared to an unmodified peptide. (G) Crystal structure (PDB 6F6D) of an unmodified histone H3 peptide in the catalytic site of KDM6B. (H) Boltz2 model of a Ksu peptide (AARK(succinyl)A) in the catalytic site of KDM6B. (I) Enrichment of JmjC-domain demethylases to the chromatin-bound proteome of HepG2/C3A cells treated with 10 mM sodium succinate for 48 hours. A -log2 p-value greater than 4 indicates significant enrichment .

    Journal: bioRxiv

    Article Title: Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states

    doi: 10.64898/2026.05.29.728167

    Figure Lengend Snippet: (A) Schematic of peptide pull-down technique used to identify potential protein interactors with histone succinylation. (B) Volcano plot of fold change and significance of enrichment of nuclear proteins from HepG2/C3A cells to a Ksu synthetic peptide vs. Kac synthetic peptide. (C) Volcano plot of fold change and significance of enrichment of bromodomain proteins to Ksu synthetic histone peptide as compared to the Kac peptide, and (D) Ksu synthetic histone peptide as compared to an unmodified peptide. (E) Volcano plot of fold change and significance of enrichment of JmjC domain demethylases to Ksu synthetic histone peptide as compared to an Kac peptide and (F) Ksu synthetic histone peptide as compared to an unmodified peptide. (G) Crystal structure (PDB 6F6D) of an unmodified histone H3 peptide in the catalytic site of KDM6B. (H) Boltz2 model of a Ksu peptide (AARK(succinyl)A) in the catalytic site of KDM6B. (I) Enrichment of JmjC-domain demethylases to the chromatin-bound proteome of HepG2/C3A cells treated with 10 mM sodium succinate for 48 hours. A -log2 p-value greater than 4 indicates significant enrichment .

    Article Snippet: The human hepatocellular carcinoma HepG2/C3A cell line was obtained from the American Type Culture Collection (ATCC, CRL-10741).

    Techniques:

    (A) Known targets of JmjC domain demethylase family enzymes. (B) Relative abundance of methylation on targets of JmjC domain demethylases in HepG2/C3A cells treated with sodium succinate. (C) Relative abundance of methylation on targets of JmjC domain demethylases in HepG2/C3A 3D cells treated with sodium succinate. (D) ChIP-MS shows co-enrichment of succinylated histone peptides with H3K27me3 peptides. Data are shown as mean ± SEM; *p-value <0 . 05, **p-value <0 . 01 in two-tailed Student’s t-test .

    Journal: bioRxiv

    Article Title: Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states

    doi: 10.64898/2026.05.29.728167

    Figure Lengend Snippet: (A) Known targets of JmjC domain demethylase family enzymes. (B) Relative abundance of methylation on targets of JmjC domain demethylases in HepG2/C3A cells treated with sodium succinate. (C) Relative abundance of methylation on targets of JmjC domain demethylases in HepG2/C3A 3D cells treated with sodium succinate. (D) ChIP-MS shows co-enrichment of succinylated histone peptides with H3K27me3 peptides. Data are shown as mean ± SEM; *p-value <0 . 05, **p-value <0 . 01 in two-tailed Student’s t-test .

    Article Snippet: The human hepatocellular carcinoma HepG2/C3A cell line was obtained from the American Type Culture Collection (ATCC, CRL-10741).

    Techniques: Methylation, Two Tailed Test

    Cell viability (%). (A) L6 myoblast cells and (B) C3A cells after 24 h of exposure to treatment as indicated.

    Journal: BioMed Research International

    Article Title: In Vitro Antidiabetic Activity and Mechanism of Action of Methanolic Extract of Opuntia stricta Cladodes

    doi: 10.1155/bmri/9325356

    Figure Lengend Snippet: Cell viability (%). (A) L6 myoblast cells and (B) C3A cells after 24 h of exposure to treatment as indicated.

    Article Snippet: The INS‐1 cells ( Rattus norvegicus , male, pancreatic β ‐cell; AddexBio, RRID:CVCL_0351), Caco‐2 cells ( Homo sapiens , male, colon epithelium; American Type Culture Collection [ATCC] HTB‐37, RRID:CVCL_0025), and L6 myoblasts ( Rattus norvegicus , male, skeletal muscle myoblast; RIKEN Cell Bank, RRID:CVCL_0385) were procured from Highveld Biological, South Africa, while the C3A subclone of HepG2 cells ( Homo sapiens , female, hepatocytes, liver tissue; ATCC CRL‐10741, RRID:CVCL_1848) was sourced from the ATCC (Manassas, Virginia, United States).

    Techniques:

    Glucose utilization (%) after 24 h of treatment in (A) L6 myoblasts and (B) C3A hepatocytes.

    Journal: BioMed Research International

    Article Title: In Vitro Antidiabetic Activity and Mechanism of Action of Methanolic Extract of Opuntia stricta Cladodes

    doi: 10.1155/bmri/9325356

    Figure Lengend Snippet: Glucose utilization (%) after 24 h of treatment in (A) L6 myoblasts and (B) C3A hepatocytes.

    Article Snippet: The INS‐1 cells ( Rattus norvegicus , male, pancreatic β ‐cell; AddexBio, RRID:CVCL_0351), Caco‐2 cells ( Homo sapiens , male, colon epithelium; American Type Culture Collection [ATCC] HTB‐37, RRID:CVCL_0025), and L6 myoblasts ( Rattus norvegicus , male, skeletal muscle myoblast; RIKEN Cell Bank, RRID:CVCL_0385) were procured from Highveld Biological, South Africa, while the C3A subclone of HepG2 cells ( Homo sapiens , female, hepatocytes, liver tissue; ATCC CRL‐10741, RRID:CVCL_1848) was sourced from the ATCC (Manassas, Virginia, United States).

    Techniques:

    Glucose uptake (%) after 4 h in (A) L6 myoblasts and (B) C3A hepatocytes following a 24‐h treatment period.

    Journal: BioMed Research International

    Article Title: In Vitro Antidiabetic Activity and Mechanism of Action of Methanolic Extract of Opuntia stricta Cladodes

    doi: 10.1155/bmri/9325356

    Figure Lengend Snippet: Glucose uptake (%) after 4 h in (A) L6 myoblasts and (B) C3A hepatocytes following a 24‐h treatment period.

    Article Snippet: The INS‐1 cells ( Rattus norvegicus , male, pancreatic β ‐cell; AddexBio, RRID:CVCL_0351), Caco‐2 cells ( Homo sapiens , male, colon epithelium; American Type Culture Collection [ATCC] HTB‐37, RRID:CVCL_0025), and L6 myoblasts ( Rattus norvegicus , male, skeletal muscle myoblast; RIKEN Cell Bank, RRID:CVCL_0385) were procured from Highveld Biological, South Africa, while the C3A subclone of HepG2 cells ( Homo sapiens , female, hepatocytes, liver tissue; ATCC CRL‐10741, RRID:CVCL_1848) was sourced from the ATCC (Manassas, Virginia, United States).

    Techniques: