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bt549 (ATCC)

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ATCC bt549
Bt549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bt549/BT-549/pm42185071-239-22-40
Average 99 stars, based on 2962 article reviews

bt549 - by Bioz Stars, 2026-09

99/100 stars

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Cell Culture:

Article Title: A Boronic Acid-Based Glutamine Analog Forms a Covalent Adduct with Kidney-Type Glutaminase and Suppresses Triple-Negative Breast Cancer Cell Proliferation
Article Snippet: The dose–response curves for ABBA and R-AST-OH (0.01–100 μM) were generated using Sigma Plot (version 10) and all experiments were performed in triplicate. .. The TNBC cell lines HCC70 (ATCC ® CRL-2315TM), HCC38 (ATCC ® CRL-2314TM), HCC1937 (ATCC ® CRL-2336TM), and BT549 (ATCC ® HTB-122TM) were obtained from ATCC (Manassas, VA, USA) and cultured under recommended conditions. ..

Article Title: Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity
Article Snippet: HEK293T (ATCC) cells were cultured in DMEM high glucose (Gibco, 11965118) with 10% FBS (Gibco) and penicillin/streptomycin (Gibco, 15140122). .. BT549 (ATCC), T47D (ATCC) and ZR-75-1 (ATCC) cells were cultured in RPMI1640 (Gibco, 11965092) supplied with 10% FBS and penicillin/streptomycin. .. MCF-7 (ATCC) cells were cultured in MEM (Gibco) supplied with 10% FBS and penicillin/streptomycin.

other:

Article Title: Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature
Article Snippet: BT549 , ATCC , HTB-122; RRID: CVCL_1092.

Multiple Displacement Amplification:

Article Title: A SIRT5-induced metabolic switch underlies chemoresistance and ATR checkpoint dependence in triple-negative breast cancer
Article Snippet: .. The human breast cancer cell lines, including Hs578T, MDA-MB-231, and BT549, are obtained from ATCC. .. These human breast cancer cell lines were cultured in RPMI medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% Pen-Strep (Gibco) at 37 °C with 5% CO2.

Article Title: NRG4 suppresses breast cancer metastasis via ERBB4-YAP1-mediated down-regulation of MMPs
Article Snippet: .. 4T1, MDA-MB-231, MDA-MB-453, MCF10A, MCF7, T47D, BT549, MDA-MB-468, and HEK293T cells were obtained from the American Type Culture Collection (Manassas, VA, USA). ..

Article Title: Triple-Negative Breast Cancer Cells Utilize IL8 and CXCL1 to Suppress NK Cells' Function and Facilitate Cancer Metastasis.
Article Snippet: .. Breast cancer cell lines (MCF-7 (RRID: CVCL_0031), T47D (RRID: CVCL_0195), BT474 (RRID: CVCL_0179), SKBR3 (RRID: CVCL_0033), UACC893 (RRID: CVCL_1782), MDA-MB-231 (RRID: CVCL_0062), BT549 (RRID: CVCL_1092), MDA-MB468 (RRID: CVCL_0419)) and human embryonic kidney 293T (RRID: CVCL_0063) cells were obtained from the American Type Culture Collection (ATCC). ..

Control:

Article Title: A human iPSC-derived sensory neuron platform for high-throughput discovery of neuroprotectants against chemotherapy-induced peripheral neuropathy.
Article Snippet: .. Several controls were employed in this experiment: cells with fresh media, no substrate, substrate with no cells, DMSO control, and positive control – 1 μM staurosporin (Tocris, cat. no. 1285) treated for 24 h. Drug response measurements in cancer cell lines MCF7, BT20, BT549 and Hs578 T breast cancer cell lines were acquired from ATCC and maintained in their recommended growth medium (DMEM for MCF7 and Hs578 T, RPMI-1640 for BT549, and EMEM for BT20) supplemented with 10% FBS and 1% penicillin/ streptomycin, and for BT-549 cells, 1 μg/mL insulin, at 37◦C and 5% CO2. ..

Positive Control:

Image Search Results

a , Representative cell cycle profiles of RPE MYC and BT549 cells after treatment with BO-264 for 72 h. The percentage of cells in G1, S, and G2-M phases of the cell cycle, as determined by DNA content based on propidium iodide staining, is indicated. b , Flow cytometry gating strategy to identify GFP-positive cells (ISRE-active cells). Representative experimental control sample, untransduced BT549 cells. Side scatter and forward scatter were used to exclude debris. Forward scatter was used to distinguish single cells. Live cells were identified as negative for live/dead staining. GFP-positive live cells were identified by setting the fluorescence threshold based on untransduced BT549 controls. For reference, GFP signal intensity from live BT549 ISRE-GFP cells treated with IFNγ is shown to the left.

Journal: bioRxiv

Article Title: MYC Overexpression Confers Sensitivity to TACC3 Inhibition for Triple-Negative Breast Cancer

doi: 10.64898/2026.04.28.721171

Figure Lengend Snippet: a , Representative cell cycle profiles of RPE MYC and BT549 cells after treatment with BO-264 for 72 h. The percentage of cells in G1, S, and G2-M phases of the cell cycle, as determined by DNA content based on propidium iodide staining, is indicated. b , Flow cytometry gating strategy to identify GFP-positive cells (ISRE-active cells). Representative experimental control sample, untransduced BT549 cells. Side scatter and forward scatter were used to exclude debris. Forward scatter was used to distinguish single cells. Live cells were identified as negative for live/dead staining. GFP-positive live cells were identified by setting the fluorescence threshold based on untransduced BT549 controls. For reference, GFP signal intensity from live BT549 ISRE-GFP cells treated with IFNγ is shown to the left.

Article Snippet: BT549 cells were obtained from the American Type Culture Collection (ATCC) and were grown according to published guidelines .

Techniques: Staining, Flow Cytometry, Control, Fluorescence

a , Immunoblot analysis of TACC3 expression in MTB/TOM and RPE cells. Representative image from n = 3 independent experiments. b , Immunoblot validation of siRNA knockdown of TACC3 in MYC OFF and MYC ON MTB/TOM cells (left) and RPE CONTROL and RPE MYC cells (right). Representative image from n = 3 independent experiments. c , Growth rate inhibition measurements of RPE CONTROL and RPE MYC cells in response to 72 h BO-264 treatment. Data show a representative experiment with n = 4 technical replicates. Error bars are mean ± s.d., and p values calculated using a two-sided t -test. Trends repeated across three independent cell passages. d , Crystal violet staining of RPE CONTROL and RPE MYC cells after 9 d treatment with increasing concentrations of BO-264. Images are representative of n = 3 independent experiments with similar results. e , Quantification of crystal violet stained RPE CONTROL and RPE MYC cells in response to various doses of BO-264. Error bars are mean ± s.e.m. P value was calculated using a two-sided t -test; * P < 0.05, *** P < 0.001, **** P < 0.0001. f , Immunoblot analysis of TACC3 proteasome-mediated degradation in RPE MYC (left) and BT549 (right) cells treated with BO-264 for 72 h. Prior to harvesting, cells were additionally incubated in the presence or absence of MG132. GAPDH is shown as a loading control. Representative image from n = 3 independent experiments.

Journal: bioRxiv

Article Title: MYC Overexpression Confers Sensitivity to TACC3 Inhibition for Triple-Negative Breast Cancer

doi: 10.64898/2026.04.28.721171

Figure Lengend Snippet: a , Immunoblot analysis of TACC3 expression in MTB/TOM and RPE cells. Representative image from n = 3 independent experiments. b , Immunoblot validation of siRNA knockdown of TACC3 in MYC OFF and MYC ON MTB/TOM cells (left) and RPE CONTROL and RPE MYC cells (right). Representative image from n = 3 independent experiments. c , Growth rate inhibition measurements of RPE CONTROL and RPE MYC cells in response to 72 h BO-264 treatment. Data show a representative experiment with n = 4 technical replicates. Error bars are mean ± s.d., and p values calculated using a two-sided t -test. Trends repeated across three independent cell passages. d , Crystal violet staining of RPE CONTROL and RPE MYC cells after 9 d treatment with increasing concentrations of BO-264. Images are representative of n = 3 independent experiments with similar results. e , Quantification of crystal violet stained RPE CONTROL and RPE MYC cells in response to various doses of BO-264. Error bars are mean ± s.e.m. P value was calculated using a two-sided t -test; * P < 0.05, *** P < 0.001, **** P < 0.0001. f , Immunoblot analysis of TACC3 proteasome-mediated degradation in RPE MYC (left) and BT549 (right) cells treated with BO-264 for 72 h. Prior to harvesting, cells were additionally incubated in the presence or absence of MG132. GAPDH is shown as a loading control. Representative image from n = 3 independent experiments.

Article Snippet: BT549 cells were obtained from the American Type Culture Collection (ATCC) and were grown according to published guidelines .

Techniques: Western Blot, Expressing, Biomarker Discovery, Knockdown, Control, Inhibition, Staining, Incubation

a , Representative image of RPE MYC cells treated with 167nM BO-264 for 72 h with DAPI staining DNA. Arrows indicate micronuclei. Higher magnification of RPE MYC cells positive for micronuclei shown below. Scale bars 10uM unless indicated otherwise. b-c , Percentage of micronucleated RPE CONTROL , RPE MYC , BT549, and 4T1 cells 72 h after treatment with vehicle or BO-264 ( b ) or transfection with non-targeting (NT) or TACC3 siRNA ( c ). Data is an average of multiple high-powered (20x) fields analyzed per sample (700-1000 total cells/sample). Data representative of n = 3 independent experiments. Error bars are mean ± s.e.m., and p value calculated using a two-sided t-test.

Journal: bioRxiv

Article Title: MYC Overexpression Confers Sensitivity to TACC3 Inhibition for Triple-Negative Breast Cancer

doi: 10.64898/2026.04.28.721171

Figure Lengend Snippet: a , Representative image of RPE MYC cells treated with 167nM BO-264 for 72 h with DAPI staining DNA. Arrows indicate micronuclei. Higher magnification of RPE MYC cells positive for micronuclei shown below. Scale bars 10uM unless indicated otherwise. b-c , Percentage of micronucleated RPE CONTROL , RPE MYC , BT549, and 4T1 cells 72 h after treatment with vehicle or BO-264 ( b ) or transfection with non-targeting (NT) or TACC3 siRNA ( c ). Data is an average of multiple high-powered (20x) fields analyzed per sample (700-1000 total cells/sample). Data representative of n = 3 independent experiments. Error bars are mean ± s.e.m., and p value calculated using a two-sided t-test.

Article Snippet: BT549 cells were obtained from the American Type Culture Collection (ATCC) and were grown according to published guidelines .

Techniques: Staining, Control, Transfection

a , Flow cytometry cell cycle analysis with PI staining in RPE CONTROL and RPE MYC cells transfected with TACC3 siRNA (left) or treated with 167nM BO-264 (right) for 72 h. b , Immunoblot analysis of G1/S progression markers in RPE CONTROL and RPE MYC cells transfected with TACC3 siRNA (left) or treated with 167nM BO-264 (right) for 72 h. β-actin is shown as a loading control. Representative image from n = 3 independent experiments. c , Flow cytometry cell cycle analysis with PI staining in MYC OFF and MYC ON MTB/TOM cells treated with 300nM BO-264 for 72 h. d , Immunoblot analysis of mitotic arrest markers in MYC OFF and MYC ON MTB/TOM cells treated with 300nM BO-264 for 72 h. Representative image from n = 3 independent experiments. e , Flow cytometry cell cycle analysis with PI staining in BT549 cells treated with 300nM BO-264 for 72 h. f , Immunoblot analysis of mitotic arrest markers in BT549 cells treated with 300nM BO-264 for 72 h. Representative image from n = 3 independent experiments. Data representative of n = 3 independent experiments. Error bars are mean ± s.e.m., and p values calculated using a two-sided t -test.

Journal: bioRxiv

Article Title: MYC Overexpression Confers Sensitivity to TACC3 Inhibition for Triple-Negative Breast Cancer

doi: 10.64898/2026.04.28.721171

Figure Lengend Snippet: a , Flow cytometry cell cycle analysis with PI staining in RPE CONTROL and RPE MYC cells transfected with TACC3 siRNA (left) or treated with 167nM BO-264 (right) for 72 h. b , Immunoblot analysis of G1/S progression markers in RPE CONTROL and RPE MYC cells transfected with TACC3 siRNA (left) or treated with 167nM BO-264 (right) for 72 h. β-actin is shown as a loading control. Representative image from n = 3 independent experiments. c , Flow cytometry cell cycle analysis with PI staining in MYC OFF and MYC ON MTB/TOM cells treated with 300nM BO-264 for 72 h. d , Immunoblot analysis of mitotic arrest markers in MYC OFF and MYC ON MTB/TOM cells treated with 300nM BO-264 for 72 h. Representative image from n = 3 independent experiments. e , Flow cytometry cell cycle analysis with PI staining in BT549 cells treated with 300nM BO-264 for 72 h. f , Immunoblot analysis of mitotic arrest markers in BT549 cells treated with 300nM BO-264 for 72 h. Representative image from n = 3 independent experiments. Data representative of n = 3 independent experiments. Error bars are mean ± s.e.m., and p values calculated using a two-sided t -test.

Article Snippet: BT549 cells were obtained from the American Type Culture Collection (ATCC) and were grown according to published guidelines .

Techniques: Flow Cytometry, Cell Cycle Assay, Staining, Control, Transfection, Western Blot

a , Diagram of IFN induction assay: BT549 cells contain an IFN reporter expressing destabilized GFP (dscGFP). ISRE: IFN-stimulated response elements. GFP median fluorescence intensity (MFI) was assessed 72 h after treatment by flow cytometry. Created with BioRender.com. b , Representative flow cytometry plot depicting GFP MFI of BT549 ISRE-GFP cells treated with vehicle, BO-264, or IFNγ for 72 h. Untransduced BT549 cells were used to establish GFP-positivity. c , Quantification of GFP positivity in BT549 ISRE-GFP cells after 72 h treatment. d , Flow cytometry analysis of MHC MFI in RPE (left) and 4T1 (right) cells treated with BO-264 for 72 h. Representative experiment with three samples per condition shown. Error bars are mean ± s.d., and p values calculated using a two-sided t -test. Trends repeated across three independent cell passages. ( e - f ), ELISA analysis of secreted CCL2 ( e ) and CXCL10 ( f ) levels in the indicated cell lines following treatment with vehicle or BO-264 for 72 h. g , ELISA analysis of intracellular cGAMP levels in the indicated cell lines upon treatment with vehicle or BO-264 for 72 h. h , Immunoblot analysis of cGAS-STING pathway activity in MTB/TOM MYC ON , 4T1, BT549, and RPE MYC cells treated with BO-264 for 72 h. β-actin is shown as a loading control. Representative image from n = 3 independent experiments. Data representative of n = 3 independent experiments. Error bars are mean ± s.e.m., and P values calculated using a two-sided t -test.

Journal: bioRxiv

Article Title: MYC Overexpression Confers Sensitivity to TACC3 Inhibition for Triple-Negative Breast Cancer

doi: 10.64898/2026.04.28.721171

Figure Lengend Snippet: a , Diagram of IFN induction assay: BT549 cells contain an IFN reporter expressing destabilized GFP (dscGFP). ISRE: IFN-stimulated response elements. GFP median fluorescence intensity (MFI) was assessed 72 h after treatment by flow cytometry. Created with BioRender.com. b , Representative flow cytometry plot depicting GFP MFI of BT549 ISRE-GFP cells treated with vehicle, BO-264, or IFNγ for 72 h. Untransduced BT549 cells were used to establish GFP-positivity. c , Quantification of GFP positivity in BT549 ISRE-GFP cells after 72 h treatment. d , Flow cytometry analysis of MHC MFI in RPE (left) and 4T1 (right) cells treated with BO-264 for 72 h. Representative experiment with three samples per condition shown. Error bars are mean ± s.d., and p values calculated using a two-sided t -test. Trends repeated across three independent cell passages. ( e - f ), ELISA analysis of secreted CCL2 ( e ) and CXCL10 ( f ) levels in the indicated cell lines following treatment with vehicle or BO-264 for 72 h. g , ELISA analysis of intracellular cGAMP levels in the indicated cell lines upon treatment with vehicle or BO-264 for 72 h. h , Immunoblot analysis of cGAS-STING pathway activity in MTB/TOM MYC ON , 4T1, BT549, and RPE MYC cells treated with BO-264 for 72 h. β-actin is shown as a loading control. Representative image from n = 3 independent experiments. Data representative of n = 3 independent experiments. Error bars are mean ± s.e.m., and P values calculated using a two-sided t -test.

Article Snippet: BT549 cells were obtained from the American Type Culture Collection (ATCC) and were grown according to published guidelines .

Techniques: Expressing, Fluorescence, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Activity Assay, Control

a , Flow cytometry analysis of DC activation (HLA-DR + CD86 + ) across six donors following DC co-culture with conditioned medium from BT549 cells treated with indicated drugs for 72h. Data are representative of n = 3 technical replicates per donor. Error bars indicate mean ± s.d., and P values were calculated using a two-sided t -test.

Journal: bioRxiv

Article Title: MYC Overexpression Confers Sensitivity to TACC3 Inhibition for Triple-Negative Breast Cancer

doi: 10.64898/2026.04.28.721171

Figure Lengend Snippet: a , Flow cytometry analysis of DC activation (HLA-DR + CD86 + ) across six donors following DC co-culture with conditioned medium from BT549 cells treated with indicated drugs for 72h. Data are representative of n = 3 technical replicates per donor. Error bars indicate mean ± s.d., and P values were calculated using a two-sided t -test.

Article Snippet: BT549 cells were obtained from the American Type Culture Collection (ATCC) and were grown according to published guidelines .

Techniques: Flow Cytometry, Activation Assay, Co-Culture Assay

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bt549 (ATCC)

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