Review



runx2  (Bioss)


Bioz Verified Symbol Bioss is a verified supplier
Bioz Manufacturer Symbol Bioss manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Bioss runx2
    The primer sequences used in this study.
    Runx2, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/runx2/product/Bioss
    Average 95 stars, based on 76 article reviews
    runx2 - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF- κ B Signaling Pathway following Stimulation of the Inflammatory Microenvironment"

    Article Title: Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF- κ B Signaling Pathway following Stimulation of the Inflammatory Microenvironment

    Journal: Stem Cells International

    doi: 10.1155/2022/1560943

    The primer sequences used in this study.
    Figure Legend Snippet: The primer sequences used in this study.

    Techniques Used:

    The inflammatory microenvironment promotes osteogenic differentiation of TSCs, and palovarotene can influence osteogenesis. (a) CCK8 proliferation assay was used to determine the dose range of palovarotene. Palovarotene at 0.5, 1, and 2 μ M concentration had less effect on TSCs proliferation. Coculture system of activated macrophages and TSCs was constructed. (b) ALP and ARS staining in TSCs after coculture with macrophages. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 50 μ m. (c) Western blot analysis and relevant quantitative analysis of OCN, SOX9, and RUNX2. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) The mRNA expression of OCN, SOX9, RUNX2, MMP-9, IL-1 β , IFN- γ , and TNF- α . Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01.
    Figure Legend Snippet: The inflammatory microenvironment promotes osteogenic differentiation of TSCs, and palovarotene can influence osteogenesis. (a) CCK8 proliferation assay was used to determine the dose range of palovarotene. Palovarotene at 0.5, 1, and 2 μ M concentration had less effect on TSCs proliferation. Coculture system of activated macrophages and TSCs was constructed. (b) ALP and ARS staining in TSCs after coculture with macrophages. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 50 μ m. (c) Western blot analysis and relevant quantitative analysis of OCN, SOX9, and RUNX2. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) The mRNA expression of OCN, SOX9, RUNX2, MMP-9, IL-1 β , IFN- γ , and TNF- α . Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01.

    Techniques Used: Proliferation Assay, Concentration Assay, Construct, Staining, Western Blot, Expressing

    Smad and NF- κ B signaling pathways play a synergistic role in HO formation, and palovarotene inhibits HO formation by blocking the Smad and NF- κ B signaling pathways. (a) The mRNA expression of OCN, SOX9, RUNX2, Smad1, Smad5, and ID1. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (b) Immunofluorescence against ID1 ( n = 3). Scale bar = 25 μ m. (c) Western blot analysis and relevant quantitative analysis of p-p65 and ID1. Data are means ± SD ( n = 3) ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) Immunofluorescence against p65 ( n = 3). Scale bar = 25 μ m. (e) Western blot analysis and relevant quantitative analysis of p65 and immunofluorescence against P65 in the previous coculture model series experiments ( n = 3). Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 25 μ m.
    Figure Legend Snippet: Smad and NF- κ B signaling pathways play a synergistic role in HO formation, and palovarotene inhibits HO formation by blocking the Smad and NF- κ B signaling pathways. (a) The mRNA expression of OCN, SOX9, RUNX2, Smad1, Smad5, and ID1. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (b) Immunofluorescence against ID1 ( n = 3). Scale bar = 25 μ m. (c) Western blot analysis and relevant quantitative analysis of p-p65 and ID1. Data are means ± SD ( n = 3) ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) Immunofluorescence against p65 ( n = 3). Scale bar = 25 μ m. (e) Western blot analysis and relevant quantitative analysis of p65 and immunofluorescence against P65 in the previous coculture model series experiments ( n = 3). Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 25 μ m.

    Techniques Used: Blocking Assay, Expressing, Immunofluorescence, Western Blot



    Similar Products

    runx2  (Bioss)
    95
    Bioss runx2
    The primer sequences used in this study.
    Runx2, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/runx2/product/Bioss
    Average 95 stars, based on 1 article reviews
    runx2 - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    The primer sequences used in this study.

    Journal: Stem Cells International

    Article Title: Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF- κ B Signaling Pathway following Stimulation of the Inflammatory Microenvironment

    doi: 10.1155/2022/1560943

    Figure Lengend Snippet: The primer sequences used in this study.

    Article Snippet: The proteins in the film were then transferred to a polyvinylidene difluoride (PVDF) membrane, using 5% skimmed milk powder at room temperature for 1 h. OCN (Servicebio, #GB11233) or RUNX2 (Bioss, #bs1134R) or SOX9 (Bioss, #bs4177R), beta actin (Servicebio, #GB11001) or P65 (Abcam, #ab179463) or Smad1/5 (Abcam, #ab38449) or Id1 (Proteintech, #18475-1--AP) or p-Smad1/5 (Abcam, #ab51451) were added to the blocking solution to dilute to the desired concentration and were incubated overnight with the membrane at 4°C.

    Techniques:

    The inflammatory microenvironment promotes osteogenic differentiation of TSCs, and palovarotene can influence osteogenesis. (a) CCK8 proliferation assay was used to determine the dose range of palovarotene. Palovarotene at 0.5, 1, and 2 μ M concentration had less effect on TSCs proliferation. Coculture system of activated macrophages and TSCs was constructed. (b) ALP and ARS staining in TSCs after coculture with macrophages. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 50 μ m. (c) Western blot analysis and relevant quantitative analysis of OCN, SOX9, and RUNX2. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) The mRNA expression of OCN, SOX9, RUNX2, MMP-9, IL-1 β , IFN- γ , and TNF- α . Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01.

    Journal: Stem Cells International

    Article Title: Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF- κ B Signaling Pathway following Stimulation of the Inflammatory Microenvironment

    doi: 10.1155/2022/1560943

    Figure Lengend Snippet: The inflammatory microenvironment promotes osteogenic differentiation of TSCs, and palovarotene can influence osteogenesis. (a) CCK8 proliferation assay was used to determine the dose range of palovarotene. Palovarotene at 0.5, 1, and 2 μ M concentration had less effect on TSCs proliferation. Coculture system of activated macrophages and TSCs was constructed. (b) ALP and ARS staining in TSCs after coculture with macrophages. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 50 μ m. (c) Western blot analysis and relevant quantitative analysis of OCN, SOX9, and RUNX2. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) The mRNA expression of OCN, SOX9, RUNX2, MMP-9, IL-1 β , IFN- γ , and TNF- α . Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01.

    Article Snippet: The proteins in the film were then transferred to a polyvinylidene difluoride (PVDF) membrane, using 5% skimmed milk powder at room temperature for 1 h. OCN (Servicebio, #GB11233) or RUNX2 (Bioss, #bs1134R) or SOX9 (Bioss, #bs4177R), beta actin (Servicebio, #GB11001) or P65 (Abcam, #ab179463) or Smad1/5 (Abcam, #ab38449) or Id1 (Proteintech, #18475-1--AP) or p-Smad1/5 (Abcam, #ab51451) were added to the blocking solution to dilute to the desired concentration and were incubated overnight with the membrane at 4°C.

    Techniques: Proliferation Assay, Concentration Assay, Construct, Staining, Western Blot, Expressing

    Smad and NF- κ B signaling pathways play a synergistic role in HO formation, and palovarotene inhibits HO formation by blocking the Smad and NF- κ B signaling pathways. (a) The mRNA expression of OCN, SOX9, RUNX2, Smad1, Smad5, and ID1. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (b) Immunofluorescence against ID1 ( n = 3). Scale bar = 25 μ m. (c) Western blot analysis and relevant quantitative analysis of p-p65 and ID1. Data are means ± SD ( n = 3) ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) Immunofluorescence against p65 ( n = 3). Scale bar = 25 μ m. (e) Western blot analysis and relevant quantitative analysis of p65 and immunofluorescence against P65 in the previous coculture model series experiments ( n = 3). Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 25 μ m.

    Journal: Stem Cells International

    Article Title: Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF- κ B Signaling Pathway following Stimulation of the Inflammatory Microenvironment

    doi: 10.1155/2022/1560943

    Figure Lengend Snippet: Smad and NF- κ B signaling pathways play a synergistic role in HO formation, and palovarotene inhibits HO formation by blocking the Smad and NF- κ B signaling pathways. (a) The mRNA expression of OCN, SOX9, RUNX2, Smad1, Smad5, and ID1. Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (b) Immunofluorescence against ID1 ( n = 3). Scale bar = 25 μ m. (c) Western blot analysis and relevant quantitative analysis of p-p65 and ID1. Data are means ± SD ( n = 3) ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. (d) Immunofluorescence against p65 ( n = 3). Scale bar = 25 μ m. (e) Western blot analysis and relevant quantitative analysis of p65 and immunofluorescence against P65 in the previous coculture model series experiments ( n = 3). Data are means ± SD ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01. # p < 0.05 and ## p < 0.01. Scale bar = 25 μ m.

    Article Snippet: The proteins in the film were then transferred to a polyvinylidene difluoride (PVDF) membrane, using 5% skimmed milk powder at room temperature for 1 h. OCN (Servicebio, #GB11233) or RUNX2 (Bioss, #bs1134R) or SOX9 (Bioss, #bs4177R), beta actin (Servicebio, #GB11001) or P65 (Abcam, #ab179463) or Smad1/5 (Abcam, #ab38449) or Id1 (Proteintech, #18475-1--AP) or p-Smad1/5 (Abcam, #ab51451) were added to the blocking solution to dilute to the desired concentration and were incubated overnight with the membrane at 4°C.

    Techniques: Blocking Assay, Expressing, Immunofluorescence, Western Blot