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cyclin e1 antibody  (Bioss)


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    Bioss cyclin e1 antibody
    G6PD, <t>Cyclin</t> <t>E1</t> and MMP9 are overexpressed in human ccRCC tissues. ( A-E ) real-time RT-PCR ( A-C ), Western blot ( D ) and grayscale scanning ( E ) were employed for the detection of G6PD, <t>Cyclin</t> <t>E1</t> and MMP9 expression levels in ccRCC tumor specimens and relevant adjacent normal tissues (n=20). β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( D ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( E ). ( F-I ) IHC were conducted to analyze the expression of G6PD, Cyclin E1 and MMP9 in ccRCC and relevant adjacent normal tissues (n=20). Representative images were shown ( F ). Statistical analysis was conducted by paired Student's t -test for Western blot analysis ( E ) and by χ 2 test for IHC analysis ( G-I ), respectively. * p <0.05, ** p <0.01 vs. Normal tissues.
    Cyclin E1 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin e1 antibody/product/Bioss
    Average 91 stars, based on 3 article reviews
    cyclin e1 antibody - by Bioz Stars, 2026-02
    91/100 stars

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    1) Product Images from "G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression"

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    Journal: International Journal of Medical Sciences

    doi: 10.7150/ijms.58902

    G6PD, Cyclin E1 and MMP9 are overexpressed in human ccRCC tissues. ( A-E ) real-time RT-PCR ( A-C ), Western blot ( D ) and grayscale scanning ( E ) were employed for the detection of G6PD, Cyclin E1 and MMP9 expression levels in ccRCC tumor specimens and relevant adjacent normal tissues (n=20). β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( D ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( E ). ( F-I ) IHC were conducted to analyze the expression of G6PD, Cyclin E1 and MMP9 in ccRCC and relevant adjacent normal tissues (n=20). Representative images were shown ( F ). Statistical analysis was conducted by paired Student's t -test for Western blot analysis ( E ) and by χ 2 test for IHC analysis ( G-I ), respectively. * p <0.05, ** p <0.01 vs. Normal tissues.
    Figure Legend Snippet: G6PD, Cyclin E1 and MMP9 are overexpressed in human ccRCC tissues. ( A-E ) real-time RT-PCR ( A-C ), Western blot ( D ) and grayscale scanning ( E ) were employed for the detection of G6PD, Cyclin E1 and MMP9 expression levels in ccRCC tumor specimens and relevant adjacent normal tissues (n=20). β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( D ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( E ). ( F-I ) IHC were conducted to analyze the expression of G6PD, Cyclin E1 and MMP9 in ccRCC and relevant adjacent normal tissues (n=20). Representative images were shown ( F ). Statistical analysis was conducted by paired Student's t -test for Western blot analysis ( E ) and by χ 2 test for IHC analysis ( G-I ), respectively. * p <0.05, ** p <0.01 vs. Normal tissues.

    Techniques Used: Quantitative RT-PCR, Western Blot, Expressing, Derivative Assay

    Cyclin E1 and MMP9 are positively correlated with G6PD and associated with poor outcomes in ccRCC patients. ( A-C ) mRNA expression levels of Cyclin D1, Cyclin E1 and MMP9 in normal kidney tissues (n=72) and ccRCC specimens (n=535) were analyzed by TCGA dataset mining (Mann-Whitney U test). ( D - F ) Spearman correlation analyses between G6PD and Cyclin D1, G6PD and Cyclin E1, G6PD and MMP9 at the mRNA expression levels were conducted in ccRCC and normal kidney tissues. ( G-O ) Kaplan-Meier analyses for overall survival of all ccRCC patients (n=528), patients with stage I/II ccRCC (n=320) and patients with stage III/IV ccRCC (n=205) in the TCGA cohort with high vs. low indicated gene mRNA expression levels were shown (log-rank test).
    Figure Legend Snippet: Cyclin E1 and MMP9 are positively correlated with G6PD and associated with poor outcomes in ccRCC patients. ( A-C ) mRNA expression levels of Cyclin D1, Cyclin E1 and MMP9 in normal kidney tissues (n=72) and ccRCC specimens (n=535) were analyzed by TCGA dataset mining (Mann-Whitney U test). ( D - F ) Spearman correlation analyses between G6PD and Cyclin D1, G6PD and Cyclin E1, G6PD and MMP9 at the mRNA expression levels were conducted in ccRCC and normal kidney tissues. ( G-O ) Kaplan-Meier analyses for overall survival of all ccRCC patients (n=528), patients with stage I/II ccRCC (n=320) and patients with stage III/IV ccRCC (n=205) in the TCGA cohort with high vs. low indicated gene mRNA expression levels were shown (log-rank test).

    Techniques Used: Expressing, MANN-WHITNEY

    Correlations between the expression of Cyclin D1, Cyclin E1, MMP9 and important clinicopathological variables in ccRCC.
    Figure Legend Snippet: Correlations between the expression of Cyclin D1, Cyclin E1, MMP9 and important clinicopathological variables in ccRCC.

    Techniques Used: Expressing

    Univariate and multivariate Cox regression analyses of the association of G6PD, Cyclin D1, Cyclin E1 and MMP9 expression and other clinicopathologic features with overall survival in ccRCC.
    Figure Legend Snippet: Univariate and multivariate Cox regression analyses of the association of G6PD, Cyclin D1, Cyclin E1 and MMP9 expression and other clinicopathologic features with overall survival in ccRCC.

    Techniques Used: Expressing

    G6PD upregulates the expression of Cyclin E1 and MMP9 in vitro . ( A-C ) The expression of Cyclin E1 and MMP9 at the mRNA and protein level in stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells was analyzed by using real-time RT-PCR ( A ), Western blot and grayscale scanning assay ( B-C ), respectively. β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were analyzed by using MMP9 activity kit in stable transfected ACHN or Caki-1 cells. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).
    Figure Legend Snippet: G6PD upregulates the expression of Cyclin E1 and MMP9 in vitro . ( A-C ) The expression of Cyclin E1 and MMP9 at the mRNA and protein level in stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells was analyzed by using real-time RT-PCR ( A ), Western blot and grayscale scanning assay ( B-C ), respectively. β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were analyzed by using MMP9 activity kit in stable transfected ACHN or Caki-1 cells. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).

    Techniques Used: Expressing, In Vitro, Stable Transfection, Transfection, Quantitative RT-PCR, Western Blot, Derivative Assay, Activity Assay

    Cyclin E1 and MMP9 are involved in the G6PD-mediated ccRCC cells proliferation and migration. ( A-B ) The expression of Cyclin E1 at the mRNA and protein level in Caki-1, ACHN-G6PD OE and relevant control cells was analyzed by using real-time RT-PCR ( A ) and Western blot assay ( B ), respectively at 48 h after Cyclin E1 siRNA transfection. β-actin was used as a protein loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). ( C-F ) Cyclin E1 siRNA transfected Caki-1, ACHN-G6PD OE and relevant control cells were subjected to cell cycle distribution analysis by PI staining and flow cytometry assay. ( G-H ) Cell proliferation abilities of Caki-1-Cyclin E1 si , ACHN-G6PD OE -Cyclin E1 si and relevant control cell lines were assessed by MTS assay at different time points. ( I-M ) Caki-1, ACHN-G6PD OE cells following treatment with the MMP9 inhibitor JNJ-0966 (10 μM, 24 h) and relevant control cells were subjected to Transwell assays. Representative images ( I , K ) and quantification analyses ( J , M ) are shown. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. relevant control (Mixed ANOVA for G-H , unpaired Student's t -test for others).
    Figure Legend Snippet: Cyclin E1 and MMP9 are involved in the G6PD-mediated ccRCC cells proliferation and migration. ( A-B ) The expression of Cyclin E1 at the mRNA and protein level in Caki-1, ACHN-G6PD OE and relevant control cells was analyzed by using real-time RT-PCR ( A ) and Western blot assay ( B ), respectively at 48 h after Cyclin E1 siRNA transfection. β-actin was used as a protein loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). ( C-F ) Cyclin E1 siRNA transfected Caki-1, ACHN-G6PD OE and relevant control cells were subjected to cell cycle distribution analysis by PI staining and flow cytometry assay. ( G-H ) Cell proliferation abilities of Caki-1-Cyclin E1 si , ACHN-G6PD OE -Cyclin E1 si and relevant control cell lines were assessed by MTS assay at different time points. ( I-M ) Caki-1, ACHN-G6PD OE cells following treatment with the MMP9 inhibitor JNJ-0966 (10 μM, 24 h) and relevant control cells were subjected to Transwell assays. Representative images ( I , K ) and quantification analyses ( J , M ) are shown. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. relevant control (Mixed ANOVA for G-H , unpaired Student's t -test for others).

    Techniques Used: Migration, Expressing, Quantitative RT-PCR, Western Blot, Transfection, Staining, Flow Cytometry, MTS Assay

    G6PD upregulates Cyclin E1 and MMP9 to enhance ccRCC progression in vivo . ( A ) Stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were subcutaneous injected in the nude mice, respectively. Representative xenografted mice images were shown. ( B-C ) The protein expression of G6PD, Cyclin E1 and MMP9 in the mice tumor tissue were analyzed by Western blot analysis ( B ) and grayscale scanning ( C ). β-actin served as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in the mice tumor tissue were analyzed by using MMP9 activity kit. The data represent three independent experiments. Each bar represented the mean ± SD. ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).
    Figure Legend Snippet: G6PD upregulates Cyclin E1 and MMP9 to enhance ccRCC progression in vivo . ( A ) Stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were subcutaneous injected in the nude mice, respectively. Representative xenografted mice images were shown. ( B-C ) The protein expression of G6PD, Cyclin E1 and MMP9 in the mice tumor tissue were analyzed by Western blot analysis ( B ) and grayscale scanning ( C ). β-actin served as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in the mice tumor tissue were analyzed by using MMP9 activity kit. The data represent three independent experiments. Each bar represented the mean ± SD. ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).

    Techniques Used: In Vivo, Stable Transfection, Transfection, Injection, Expressing, Western Blot, Derivative Assay, Activity Assay



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    G6PD, <t>Cyclin</t> <t>E1</t> and MMP9 are overexpressed in human ccRCC tissues. ( A-E ) real-time RT-PCR ( A-C ), Western blot ( D ) and grayscale scanning ( E ) were employed for the detection of G6PD, <t>Cyclin</t> <t>E1</t> and MMP9 expression levels in ccRCC tumor specimens and relevant adjacent normal tissues (n=20). β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( D ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( E ). ( F-I ) IHC were conducted to analyze the expression of G6PD, Cyclin E1 and MMP9 in ccRCC and relevant adjacent normal tissues (n=20). Representative images were shown ( F ). Statistical analysis was conducted by paired Student's t -test for Western blot analysis ( E ) and by χ 2 test for IHC analysis ( G-I ), respectively. * p <0.05, ** p <0.01 vs. Normal tissues.
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    G6PD, Cyclin E1 and MMP9 are overexpressed in human ccRCC tissues. ( A-E ) real-time RT-PCR ( A-C ), Western blot ( D ) and grayscale scanning ( E ) were employed for the detection of G6PD, Cyclin E1 and MMP9 expression levels in ccRCC tumor specimens and relevant adjacent normal tissues (n=20). β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( D ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( E ). ( F-I ) IHC were conducted to analyze the expression of G6PD, Cyclin E1 and MMP9 in ccRCC and relevant adjacent normal tissues (n=20). Representative images were shown ( F ). Statistical analysis was conducted by paired Student's t -test for Western blot analysis ( E ) and by χ 2 test for IHC analysis ( G-I ), respectively. * p <0.05, ** p <0.01 vs. Normal tissues.

    Journal: International Journal of Medical Sciences

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    doi: 10.7150/ijms.58902

    Figure Lengend Snippet: G6PD, Cyclin E1 and MMP9 are overexpressed in human ccRCC tissues. ( A-E ) real-time RT-PCR ( A-C ), Western blot ( D ) and grayscale scanning ( E ) were employed for the detection of G6PD, Cyclin E1 and MMP9 expression levels in ccRCC tumor specimens and relevant adjacent normal tissues (n=20). β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( D ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( E ). ( F-I ) IHC were conducted to analyze the expression of G6PD, Cyclin E1 and MMP9 in ccRCC and relevant adjacent normal tissues (n=20). Representative images were shown ( F ). Statistical analysis was conducted by paired Student's t -test for Western blot analysis ( E ) and by χ 2 test for IHC analysis ( G-I ), respectively. * p <0.05, ** p <0.01 vs. Normal tissues.

    Article Snippet: The following antibodies were used: G6PD antibody (ab133525, Abcam), Cyclin E1 antibody (bsm-52048R, Bioss, Beijing, China), MMP9 antibody (ab76003, Abcam).

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Derivative Assay

    Cyclin E1 and MMP9 are positively correlated with G6PD and associated with poor outcomes in ccRCC patients. ( A-C ) mRNA expression levels of Cyclin D1, Cyclin E1 and MMP9 in normal kidney tissues (n=72) and ccRCC specimens (n=535) were analyzed by TCGA dataset mining (Mann-Whitney U test). ( D - F ) Spearman correlation analyses between G6PD and Cyclin D1, G6PD and Cyclin E1, G6PD and MMP9 at the mRNA expression levels were conducted in ccRCC and normal kidney tissues. ( G-O ) Kaplan-Meier analyses for overall survival of all ccRCC patients (n=528), patients with stage I/II ccRCC (n=320) and patients with stage III/IV ccRCC (n=205) in the TCGA cohort with high vs. low indicated gene mRNA expression levels were shown (log-rank test).

    Journal: International Journal of Medical Sciences

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    doi: 10.7150/ijms.58902

    Figure Lengend Snippet: Cyclin E1 and MMP9 are positively correlated with G6PD and associated with poor outcomes in ccRCC patients. ( A-C ) mRNA expression levels of Cyclin D1, Cyclin E1 and MMP9 in normal kidney tissues (n=72) and ccRCC specimens (n=535) were analyzed by TCGA dataset mining (Mann-Whitney U test). ( D - F ) Spearman correlation analyses between G6PD and Cyclin D1, G6PD and Cyclin E1, G6PD and MMP9 at the mRNA expression levels were conducted in ccRCC and normal kidney tissues. ( G-O ) Kaplan-Meier analyses for overall survival of all ccRCC patients (n=528), patients with stage I/II ccRCC (n=320) and patients with stage III/IV ccRCC (n=205) in the TCGA cohort with high vs. low indicated gene mRNA expression levels were shown (log-rank test).

    Article Snippet: The following antibodies were used: G6PD antibody (ab133525, Abcam), Cyclin E1 antibody (bsm-52048R, Bioss, Beijing, China), MMP9 antibody (ab76003, Abcam).

    Techniques: Expressing, MANN-WHITNEY

    Correlations between the expression of Cyclin D1, Cyclin E1, MMP9 and important clinicopathological variables in ccRCC.

    Journal: International Journal of Medical Sciences

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    doi: 10.7150/ijms.58902

    Figure Lengend Snippet: Correlations between the expression of Cyclin D1, Cyclin E1, MMP9 and important clinicopathological variables in ccRCC.

    Article Snippet: The following antibodies were used: G6PD antibody (ab133525, Abcam), Cyclin E1 antibody (bsm-52048R, Bioss, Beijing, China), MMP9 antibody (ab76003, Abcam).

    Techniques: Expressing

    Univariate and multivariate Cox regression analyses of the association of G6PD, Cyclin D1, Cyclin E1 and MMP9 expression and other clinicopathologic features with overall survival in ccRCC.

    Journal: International Journal of Medical Sciences

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    doi: 10.7150/ijms.58902

    Figure Lengend Snippet: Univariate and multivariate Cox regression analyses of the association of G6PD, Cyclin D1, Cyclin E1 and MMP9 expression and other clinicopathologic features with overall survival in ccRCC.

    Article Snippet: The following antibodies were used: G6PD antibody (ab133525, Abcam), Cyclin E1 antibody (bsm-52048R, Bioss, Beijing, China), MMP9 antibody (ab76003, Abcam).

    Techniques: Expressing

    G6PD upregulates the expression of Cyclin E1 and MMP9 in vitro . ( A-C ) The expression of Cyclin E1 and MMP9 at the mRNA and protein level in stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells was analyzed by using real-time RT-PCR ( A ), Western blot and grayscale scanning assay ( B-C ), respectively. β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were analyzed by using MMP9 activity kit in stable transfected ACHN or Caki-1 cells. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).

    Journal: International Journal of Medical Sciences

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    doi: 10.7150/ijms.58902

    Figure Lengend Snippet: G6PD upregulates the expression of Cyclin E1 and MMP9 in vitro . ( A-C ) The expression of Cyclin E1 and MMP9 at the mRNA and protein level in stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells was analyzed by using real-time RT-PCR ( A ), Western blot and grayscale scanning assay ( B-C ), respectively. β-actin was used as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were analyzed by using MMP9 activity kit in stable transfected ACHN or Caki-1 cells. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).

    Article Snippet: The following antibodies were used: G6PD antibody (ab133525, Abcam), Cyclin E1 antibody (bsm-52048R, Bioss, Beijing, China), MMP9 antibody (ab76003, Abcam).

    Techniques: Expressing, In Vitro, Stable Transfection, Transfection, Quantitative RT-PCR, Western Blot, Derivative Assay, Activity Assay

    Cyclin E1 and MMP9 are involved in the G6PD-mediated ccRCC cells proliferation and migration. ( A-B ) The expression of Cyclin E1 at the mRNA and protein level in Caki-1, ACHN-G6PD OE and relevant control cells was analyzed by using real-time RT-PCR ( A ) and Western blot assay ( B ), respectively at 48 h after Cyclin E1 siRNA transfection. β-actin was used as a protein loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). ( C-F ) Cyclin E1 siRNA transfected Caki-1, ACHN-G6PD OE and relevant control cells were subjected to cell cycle distribution analysis by PI staining and flow cytometry assay. ( G-H ) Cell proliferation abilities of Caki-1-Cyclin E1 si , ACHN-G6PD OE -Cyclin E1 si and relevant control cell lines were assessed by MTS assay at different time points. ( I-M ) Caki-1, ACHN-G6PD OE cells following treatment with the MMP9 inhibitor JNJ-0966 (10 μM, 24 h) and relevant control cells were subjected to Transwell assays. Representative images ( I , K ) and quantification analyses ( J , M ) are shown. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. relevant control (Mixed ANOVA for G-H , unpaired Student's t -test for others).

    Journal: International Journal of Medical Sciences

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    doi: 10.7150/ijms.58902

    Figure Lengend Snippet: Cyclin E1 and MMP9 are involved in the G6PD-mediated ccRCC cells proliferation and migration. ( A-B ) The expression of Cyclin E1 at the mRNA and protein level in Caki-1, ACHN-G6PD OE and relevant control cells was analyzed by using real-time RT-PCR ( A ) and Western blot assay ( B ), respectively at 48 h after Cyclin E1 siRNA transfection. β-actin was used as a protein loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). ( C-F ) Cyclin E1 siRNA transfected Caki-1, ACHN-G6PD OE and relevant control cells were subjected to cell cycle distribution analysis by PI staining and flow cytometry assay. ( G-H ) Cell proliferation abilities of Caki-1-Cyclin E1 si , ACHN-G6PD OE -Cyclin E1 si and relevant control cell lines were assessed by MTS assay at different time points. ( I-M ) Caki-1, ACHN-G6PD OE cells following treatment with the MMP9 inhibitor JNJ-0966 (10 μM, 24 h) and relevant control cells were subjected to Transwell assays. Representative images ( I , K ) and quantification analyses ( J , M ) are shown. All assays were done in at least triplicate. Bars represent the means ± SD. * p <0.05, ** p <0.01, *** p <0.001 vs. relevant control (Mixed ANOVA for G-H , unpaired Student's t -test for others).

    Article Snippet: The following antibodies were used: G6PD antibody (ab133525, Abcam), Cyclin E1 antibody (bsm-52048R, Bioss, Beijing, China), MMP9 antibody (ab76003, Abcam).

    Techniques: Migration, Expressing, Quantitative RT-PCR, Western Blot, Transfection, Staining, Flow Cytometry, MTS Assay

    G6PD upregulates Cyclin E1 and MMP9 to enhance ccRCC progression in vivo . ( A ) Stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were subcutaneous injected in the nude mice, respectively. Representative xenografted mice images were shown. ( B-C ) The protein expression of G6PD, Cyclin E1 and MMP9 in the mice tumor tissue were analyzed by Western blot analysis ( B ) and grayscale scanning ( C ). β-actin served as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in the mice tumor tissue were analyzed by using MMP9 activity kit. The data represent three independent experiments. Each bar represented the mean ± SD. ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).

    Journal: International Journal of Medical Sciences

    Article Title: G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression

    doi: 10.7150/ijms.58902

    Figure Lengend Snippet: G6PD upregulates Cyclin E1 and MMP9 to enhance ccRCC progression in vivo . ( A ) Stably transfected ACHN-G6PD OE , Caki-1- G6PD si and relevant control cells were subcutaneous injected in the nude mice, respectively. Representative xenografted mice images were shown. ( B-C ) The protein expression of G6PD, Cyclin E1 and MMP9 in the mice tumor tissue were analyzed by Western blot analysis ( B ) and grayscale scanning ( C ). β-actin served as a loading control. Representative cropped gels and blots of the Western blot analysis were shown ( B ). The samples used for quantitative comparisons in the Western blot analysis were derived from the same experiment and that gels were processed in parallel ( C ). ( D ) Relative MMP9 enzyme activities in the mice tumor tissue were analyzed by using MMP9 activity kit. The data represent three independent experiments. Each bar represented the mean ± SD. ** p <0.01, *** p <0.001 vs. Control or Non-silencer (unpaired Student's t -test).

    Article Snippet: The following antibodies were used: G6PD antibody (ab133525, Abcam), Cyclin E1 antibody (bsm-52048R, Bioss, Beijing, China), MMP9 antibody (ab76003, Abcam).

    Techniques: In Vivo, Stable Transfection, Transfection, Injection, Expressing, Western Blot, Derivative Assay, Activity Assay