Journal: International Journal of Clinical and Experimental Pathology
Article Title: Preventive effects of transplantation of oral mucosal epithelial cells seeded on a decellularized amniotic membrane in a model of intrauterine adhesion
doi:
Figure Lengend Snippet: Immunohistochemical staining of CK-18 and HMW-CK in the dHAM+OMECs group. Endometrial epithelium and endometrial glands were positive for CK-18. However, 14 and 28 days after surgery in the dHAM+OMECs transplantation group, the regenerated epithelium was also observed and was positive for CK-18. Immunohistochemical staining of HMW-CK also revealed a positive expression of OMECs, but not endometrium and endometrial glands in the dHAM+OMECs transplantation group.
Article Snippet: The slices were immersed in 3% hydrogen peroxide solution for 15 min to block endogenous peroxidase before being incubated overnight at 4°C with primary antibodies including anti-cytokeratin 18 (CK-18) (bsm-33103M, Bioss) and anti-high molecular weight cytokeratin (anti-HMW-CK) (bs-1494R, Bioss).
Techniques: Immunohistochemical staining, Staining, Transplantation Assay, Expressing