anti aqp0 antibodies conjugated with af647  (Bioss)

Journal: Stem Cell Research & Therapy

Article Title: AQP0 is a novel surface marker for deciphering abnormal erythropoiesis

doi: 10.1186/s13287-021-02343-4

Figure Lengend Snippet: Characterization of mouse erythropoiesis stages using three surface markers (CD71, TER119, and Aqp0). Flow cytometry analysis of mouse erythropoiesis ( a ). Mouse bone marrow cells were isolated and stained with anti-CD71 antibodies conjugated with FITC, anti-TER119 antibodies conjugated with PE/Cy7, and anti-Aqp0 antibodies conjugated with Alexa Fluor 647. The percentages of positive cells of each antibody in unstained control were showed on the top. CD71 −/dim /TER119 −/dim cells were gated as nonerythroid cells. CD71 high /TER119 low , CD71 high /TER119 high , CD71 dim /TER119 high , and CD71 − /TER119 high were defined as region 1 (R1), region 2 (R2), region 3 (R3), and region 4 (R4), respectively. R3 was gated, and the relationship between FSC (cell size) and Aqp0 expression was analyzed. The percentages of Aqp0 + cells in each population were analyzed and quantified ( b ). Data are representative of four independent experiments and reported as the mean ± standard deviation

Article Snippet: Cells were then stained with anti-CD71 antibodies conjugated with FITC, anti-TER119 antibodies conjugated with PE/Cy7 (BioLegend), and anti-Aqp0 antibodies conjugated with AF647 (Bioss) for 1 h at room temperature.

Techniques: Flow Cytometry, Isolation, Staining, Expressing, Standard Deviation

Journal: Stem Cell Research & Therapy

Article Title: AQP0 is a novel surface marker for deciphering abnormal erythropoiesis

doi: 10.1186/s13287-021-02343-4

Figure Lengend Snippet: Relative mRNA expressions of erythroid specific transcription factors in each erythroid differentiation stage. Mouse bone marrow cells were isolated and categorized as R1, R2, R3/Aqp0 − , R3/Aqp0 + , R4, and nonerythroid populations on the basis of the fluorescence intensity of three erythroid specific cell surface markers (CD71, TER119, and Aqp0). The mRNA expression level of each gene in the R1 region was normalized to one-fold. Relative folds of mRNA expression of Klf1 ( a ), Nfe2 ( b ), and Gfi1b ( c ) in each cell population relative to R1 were analyzed using a quantitative reverse polymerase chain reaction assay. Data are representative of three independent experiments and reported as the mean ± standard deviation. * P < 0.05 and ** P < 0.01 relative to R1 groups

Article Snippet: Cells were then stained with anti-CD71 antibodies conjugated with FITC, anti-TER119 antibodies conjugated with PE/Cy7 (BioLegend), and anti-Aqp0 antibodies conjugated with AF647 (Bioss) for 1 h at room temperature.

Techniques: Isolation, Fluorescence, Expressing, Polymerase Chain Reaction, Standard Deviation

Journal: Stem Cell Research & Therapy

Article Title: AQP0 is a novel surface marker for deciphering abnormal erythropoiesis

doi: 10.1186/s13287-021-02343-4

Figure Lengend Snippet: Characterization of human erythropoiesis stages using three surface markers (CD71, CD235a, and AQP0). Flow cytometry analysis of human erythropoiesis ( a ). Human bone marrow cells were isolated and stained with anti-CD71 antibodies conjugated with PE/Cy7, anti-CD235a antibodies conjugated with FITC, and anti-AQP0 antibodies conjugated with Alexa Fluor 647. The percentages of positive cells of each antibody in unstained control were showed on the top. CD71 −/dim /CD235a −/dim cells were gated as nonerythroid cells. CD71 high /CD235a low , CD71 high /CD235a high , CD71 dim /CD235a high , and CD71 − /CD235a high were defined as region 1 (R1), region 2 (R2), region 3 (R3), and region 4 (R4), respectively. R3 was gated, and the relationship between FSC (cell size) and AQP0 expression was analyzed. The percentages of AQP0 high cells in each population were analyzed and quantified ( b ). The percentages of AQP0 low and AQP0 high cells in R3 were shown ( c ). The data are representative of three independent experiments and reported as the mean ± standard deviation

Article Snippet: Cells were then stained with anti-CD71 antibodies conjugated with FITC, anti-TER119 antibodies conjugated with PE/Cy7 (BioLegend), and anti-Aqp0 antibodies conjugated with AF647 (Bioss) for 1 h at room temperature.

Techniques: Flow Cytometry, Isolation, Staining, Expressing, Standard Deviation

Journal: Stem Cell Research & Therapy

Article Title: AQP0 is a novel surface marker for deciphering abnormal erythropoiesis

doi: 10.1186/s13287-021-02343-4

Figure Lengend Snippet: Characterization of erythropoiesis of patients using three surface markers (CD71, CD235a, and AQP0). Clinical complete blood count values of nine patients ( a ). The bone marrow cells from nine patients were isolated and stained with anti-CD71 antibodies conjugated with PE/Cy7, anti-CD235a antibodies conjugated with FITC, and anti-AQP0 antibodies conjugated with Alexa Fluor 647. CD71 −/dim /CD235a −/dim cells were gated as nonerythroid cells. CD71 high /CD235a low , CD71 high /CD235a high , CD71 dim /CD235a high , and CD71 − /CD235a high were defined as region 1 (R1), region 2 (R2), region 3 (R3), and region 4 (R4), respectively. R3 was gated, and the relationship between FSC (cell size) and AQP0 expression was analyzed. The percentages of AQP0 high cells in each population were analyzed and quantified in patient 1 ( b ), patient 2 ( c ), patient 3 ( d ), patient 4 ( e ), patient 5 ( f ), patient 6 ( g ), patient 7 ( h ), patient 8 ( i ), and patient 9 ( j ). The data are representative of one independent experiment

Article Snippet: Cells were then stained with anti-CD71 antibodies conjugated with FITC, anti-TER119 antibodies conjugated with PE/Cy7 (BioLegend), and anti-Aqp0 antibodies conjugated with AF647 (Bioss) for 1 h at room temperature.

Techniques: Isolation, Staining, Expressing