Journal: Cells
Article Title: Achyranthis radix Extract Enhances Antioxidant Effect of Placenta-Derived Mesenchymal Stem Cell on Injured Human Ocular Cells
doi: 10.3390/cells13141229
Figure Lengend Snippet: ARE-primed PD-MSC increased RPE65 and RDN11. ( A ) The localization of RDN11 analyzed by IF staining. ( B ) The RDN11 expression quantified by image J program. The gray bar, gray hatch bar, black bar, black hatch bar means ARPE19 cells with H 2 O 2 and ARE low, ARPE19 cells with with H 2 O 2, ARE low and PD-MSCs, ARPE19 cells with H 2 O 2 and ARE high and ARPE19 cells with with H 2 O 2, ARE high and PD-MSCs. The mRNA levels of ( C ) RPE65 and ( E ) RDN11 analyzed by qRT-PCR. The protein levels of ( D ) RPE65 and ( F ) RDN11 analyzed by Western blot. Scale bar = 200 μm. Each experiment was replicated 5 times, resulting in a total sample size of 5 ( n = 5). The data represent the mean ± SEM. Statistical significance was determined by using one-way ANOVA and Tukey’s post hoc test for the comparison of groups, * p < 0.05.
Article Snippet: Primary antibodies were used as follows: anti-HMOX1 (1:1000, NBP1-97507, Novus Biologicals, Centennial, CO, USA) [ ], anti-SOD1 (1:1000, 4266S, Cell Signaling Technology, Danvers, MA, USA) [ ], anti-TGFB1 (1:1000, ab92486, Abcam, Cambridge, UK) [ ], anti-IL6 (1:1000, ab6672, Abcam) [ ]), anti-RPE65 (1:2000, MA1-16578, Invitrogen) [ ], anti-RDN11 (1:1000, bs-6214R, Bioss, Woburn, MA, USA), anti-total AKT (1:1000, 9272S, Cell Signaling Technology) [ ], anti-phospho AKT (1:1000, 9271S, Cell Signaling Technology) [ ], anti-PI3K (1:2000, 4255S, Cell Signaling Technology) [ ], and anti-GAPDH (1:3000, LF-PA0018, AbFrontier, Seoul, Republic of Korea) [ ].
Techniques: Staining, Expressing, Quantitative RT-PCR, Western Blot, Comparison
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