pigr  (Bioss)

Journal: Animals : an Open Access Journal from MDPI

Article Title: Porcine Milk-Derived Small Extracellular Vesicles Promote Intestinal Immunoglobulin Production through pIgR

doi: 10.3390/ani11061522

Figure Lengend Snippet: Identification of PM-sEVs and effect of PM-sEVs on intestinal SIgA levels in mice. ( A ) Morphology of PM-sEVs visualized under TEM. Scale bar: 500 nm. The arrows indicate “PM-sEVs”. ( B ) Size distribution analysis of PM-sEVs. ( C ) Western Blotting analysis of porcine milk somatic cell (PM-SC) and PM-sEVs. Milk SC and PM-sEVs were blotted for commonly used sEVs markers TSG101, CD63, and Alix and the endoplasmic reticulum marker Calnexin. ( D ) ELISA analysis of SIgA levels of intestinal luminal contents in mice ( n = 8). ( E ) qRT-PCR analysis of pIgR mRNA expression levels of intestinal tissue in mice ( n = 8). ( F , G ) Western Blotting analysis of pIgR protein level of intestinal tissue in mice ( n = 4). ( H ) Intestine of mice was sectioned for immunofluorescence staining (Scale bar = 100 μm) to analysis the level of IgA ( n = 4). All data are presented as means ± SEM. * p < 0.05; ** p < 0.01.

Article Snippet: The following antibodies were used: anti-CD63, anti-TSG101, anti-Alix, and anti-Calnexin (Sangon Biotech, Shanghai, China), anti-pIgR-mice (AF2800-SP, R&D Systems, Minneapolis, MN, USA), anti-pIgR-pig (bs-6061R, Bioss, Beijing, China), and anti-β-actin (AP0060, Bioworld, Technology Inc., Bloomington, MN, USA).

Techniques: Western Blot, Marker, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Immunofluorescence, Staining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Porcine Milk-Derived Small Extracellular Vesicles Promote Intestinal Immunoglobulin Production through pIgR

doi: 10.3390/ani11061522

Figure Lengend Snippet: Effects of PM-sEVs on noncoding RNA expression in IPEC-J2 cell and piglet intestine. ( A ) The potential interaction among pIgR, miRNAs, and PM-sEVs circRNAs. ( B ) qRT-PCR analysis of miR-221-5p, miR-133a-3p, miR-383, and miR-370 expression levels in IPEC-J2 cell ( n = 6). ( C ) qRT-PCR analysis of circ-XPO4, circ-SUGCT, and circ-CTT3 expression levels in IPEC-J2 cell ( n = 6). ( D ) PCR and agarose gel electrophoresis identify PCR product size of circ-XPO4, “→←” represent convergent primers, “←→” represent divergent primers. ( E ) Sanger sequencing confirm the splice junctions of circ-XPO4, the arrow points to the splice junctions. ( F ) PCR and agarose gel electrophoresis analysis the abundance of circ-XPO4 and linear mRNA (β-actin) treated with RNase R. ( G , H ) qRT-PCR analysis of circ-XPO4 and miR-221-5p expression levels in intestinal tissue of piglet ( n = 6). NS, represent non-significant ( p > 0.05); * p < 0.05. All data are presented as means ± SEM.

Article Snippet: The following antibodies were used: anti-CD63, anti-TSG101, anti-Alix, and anti-Calnexin (Sangon Biotech, Shanghai, China), anti-pIgR-mice (AF2800-SP, R&D Systems, Minneapolis, MN, USA), anti-pIgR-pig (bs-6061R, Bioss, Beijing, China), and anti-β-actin (AP0060, Bioworld, Technology Inc., Bloomington, MN, USA).

Techniques: RNA Expression, Quantitative RT-PCR, Expressing, Agarose Gel Electrophoresis, Sequencing

Journal: bioRxiv

Article Title: Effect of hydrogen inhalation on IL-40 and SIgA in a Rat Model of Pulmonary Mucosal Immunity

doi: 10.1101/2020.06.29.177345

Figure Lengend Snippet: Effect of hydrogen on the expression of SIGA and PIgR in the lung obtained from the COPD rat model, determined by immunohistochemical staining or Western blotting. A Immunohistochemical staining of the lung sections for SIGA, and Western blotting of the lung sections for PIgR. B Quantitative analysis of the expression of SIGA and PIgR in the COPD rat model. (n=10 for each group. ## P <0.01 compared with COPD group; ** P <0.01). Each experiment was repeated three times and similar results were obtained.

Article Snippet: Nitrogen (15% O 2 , 85% N 2 ) was purchased from Shijiazhuang Central Plains Specialty Gases Ltd., and a 2% H 2 gas mixture (21% O 2 , 2% H 2 , 77% N 2 ) was purchased from Guangzhou Puyuan Gas Company Ltd. Anti-IgA secretory component (Mouse, ab212330, 1:500 in IHC, Abcam), anti-IL-4 (Mouse, sc53084, 1:100 in IHC, 1:500 in WB; SanTaCruz), anti-PIGR (Rabbit, bs-6061R, 1:500 in WB; Bioss), anti-IL-5 (Rabbit, GTX55678, 1:100 in IHC, 1:500 in WB; GeneTex), anti-TGF-β1 (Rabbit, ARG56429, 1:500 in WB; Arigo), anti-C17orf99 (Rabbit, PA5-71295, 1:500 in WB; ThermoFisher) primary antibodies, and anti-rabbit and anti-mouse secondary antibodies (Abbkine, Reddlands, CA, USA) were used to determine protein expression.

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot