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col2  (Bioss)


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    Structured Review

    Bioss col2
    A , B Western blot analysis of the expression levels of apoptosis-related proteins PERK, MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. C , D Representative images of TUNEL staining and quantitative statistical analysis showing the proportion of TUNEL-positive cells in each group. The scale bar is 100 μm. E , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G mRNA levels of PERK, NOXA, BAX, and MMP13 in NPCs from each group. H , I Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, <t>COL2,</t> MMP13, and MMP3 in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Col2, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/col2/product/Bioss
    Average 92 stars, based on 5 article reviews
    col2 - by Bioz Stars, 2026-02
    92/100 stars

    Images

    1) Product Images from "NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation"

    Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-025-02539-0

    A , B Western blot analysis of the expression levels of apoptosis-related proteins PERK, MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. C , D Representative images of TUNEL staining and quantitative statistical analysis showing the proportion of TUNEL-positive cells in each group. The scale bar is 100 μm. E , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G mRNA levels of PERK, NOXA, BAX, and MMP13 in NPCs from each group. H , I Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: A , B Western blot analysis of the expression levels of apoptosis-related proteins PERK, MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. C , D Representative images of TUNEL staining and quantitative statistical analysis showing the proportion of TUNEL-positive cells in each group. The scale bar is 100 μm. E , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G mRNA levels of PERK, NOXA, BAX, and MMP13 in NPCs from each group. H , I Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane

    A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs GAPDH was used as an internal control. C , E Representative images of TUNEL staining and quantitative statistical analysis showed each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs GAPDH was used as an internal control. C , E Representative images of TUNEL staining and quantitative statistical analysis showed each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane, Flow Cytometry

    A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs, GAPDH was used as an internal control. C , F Representative images of TUNEL staining and quantitative statistical analysis show each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , E Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Representative images and quantitative statistical analysis of the expression of NOXA, BAX, ACAN, and MMP3 in each group by cellular immunofluorescence. The scale bar is 100 μm. K , L Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs, GAPDH was used as an internal control. C , F Representative images of TUNEL staining and quantitative statistical analysis show each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , E Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Representative images and quantitative statistical analysis of the expression of NOXA, BAX, ACAN, and MMP3 in each group by cellular immunofluorescence. The scale bar is 100 μm. K , L Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane, Flow Cytometry, Immunofluorescence



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    Image Search Results


    A , B Western blot analysis of the expression levels of apoptosis-related proteins PERK, MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. C , D Representative images of TUNEL staining and quantitative statistical analysis showing the proportion of TUNEL-positive cells in each group. The scale bar is 100 μm. E , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G mRNA levels of PERK, NOXA, BAX, and MMP13 in NPCs from each group. H , I Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Cell Death Discovery

    Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

    doi: 10.1038/s41420-025-02539-0

    Figure Lengend Snippet: A , B Western blot analysis of the expression levels of apoptosis-related proteins PERK, MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. C , D Representative images of TUNEL staining and quantitative statistical analysis showing the proportion of TUNEL-positive cells in each group. The scale bar is 100 μm. E , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G mRNA levels of PERK, NOXA, BAX, and MMP13 in NPCs from each group. H , I Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs after treatment with TG and TG + 4-PBA. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The first antibodies were incubated overnight at 4 °C with the following dilutions: NOXA (ab222852, 1:1000, Abcam, Cambridge, UK), PERK (bs-2469R, 1:1000, Bioss, Beijing, China), BAX (bs-0127R, 1:1000, Bioss, Beijing, China), BCL-2 (bs-0032R, 1:2000, Bioss, Beijing, China), MCL-1 (16225-1-AP, 1:1000, Proteintech, Wuhan, China), MMP3 (17873-1-AP, 1:1000, Proteintech, Wuhan, China), MMP13 (18165-1-AP, 1:1000, Proteintech, Wuhan, China), Cleaved Caspase-3 (25128-1-AP, 1:1000, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:1000, Bioss, Beijing, China), COL2 (bs-5881R, 1:1000, Bioss, Beijing, China), GAPDH (10494-1-AP, 1:5000, Proteintech, Wuhan, China).

    Techniques: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane

    A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs GAPDH was used as an internal control. C , E Representative images of TUNEL staining and quantitative statistical analysis showed each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Cell Death Discovery

    Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

    doi: 10.1038/s41420-025-02539-0

    Figure Lengend Snippet: A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs GAPDH was used as an internal control. C , E Representative images of TUNEL staining and quantitative statistical analysis showed each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , F Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The first antibodies were incubated overnight at 4 °C with the following dilutions: NOXA (ab222852, 1:1000, Abcam, Cambridge, UK), PERK (bs-2469R, 1:1000, Bioss, Beijing, China), BAX (bs-0127R, 1:1000, Bioss, Beijing, China), BCL-2 (bs-0032R, 1:2000, Bioss, Beijing, China), MCL-1 (16225-1-AP, 1:1000, Proteintech, Wuhan, China), MMP3 (17873-1-AP, 1:1000, Proteintech, Wuhan, China), MMP13 (18165-1-AP, 1:1000, Proteintech, Wuhan, China), Cleaved Caspase-3 (25128-1-AP, 1:1000, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:1000, Bioss, Beijing, China), COL2 (bs-5881R, 1:1000, Bioss, Beijing, China), GAPDH (10494-1-AP, 1:5000, Proteintech, Wuhan, China).

    Techniques: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane, Flow Cytometry

    A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs, GAPDH was used as an internal control. C , F Representative images of TUNEL staining and quantitative statistical analysis show each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , E Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Representative images and quantitative statistical analysis of the expression of NOXA, BAX, ACAN, and MMP3 in each group by cellular immunofluorescence. The scale bar is 100 μm. K , L Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Cell Death Discovery

    Article Title: NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

    doi: 10.1038/s41420-025-02539-0

    Figure Lengend Snippet: A , B Western blot analysis of the expression levels of apoptosis-related proteins MCL-1, BCL-2, BAX, C-CAS3, and NOXA in NPCs, GAPDH was used as an internal control. C , F Representative images of TUNEL staining and quantitative statistical analysis show each group’s proportion of TUNEL-positive cells. The scale bar is 100 μm. D , E Representative images of JC-1 staining for mitochondrial membrane potential and quantitative statistical analysis. The scale bar is 100 μm. G , H Representative flow cytometry scatter plot and quantitative analysis of apoptosis rates in NPCs of each group. I , J Representative images and quantitative statistical analysis of the expression of NOXA, BAX, ACAN, and MMP3 in each group by cellular immunofluorescence. The scale bar is 100 μm. K , L Western blot analysis of the expression levels of ECM degradation-related proteins ACAN, COL2, MMP13, and MMP3 in NPCs. GAPDH was used as an internal control. Data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The first antibodies were incubated overnight at 4 °C with the following dilutions: NOXA (ab222852, 1:1000, Abcam, Cambridge, UK), PERK (bs-2469R, 1:1000, Bioss, Beijing, China), BAX (bs-0127R, 1:1000, Bioss, Beijing, China), BCL-2 (bs-0032R, 1:2000, Bioss, Beijing, China), MCL-1 (16225-1-AP, 1:1000, Proteintech, Wuhan, China), MMP3 (17873-1-AP, 1:1000, Proteintech, Wuhan, China), MMP13 (18165-1-AP, 1:1000, Proteintech, Wuhan, China), Cleaved Caspase-3 (25128-1-AP, 1:1000, Proteintech, Wuhan, China), ACAN (bs-1223R, 1:1000, Bioss, Beijing, China), COL2 (bs-5881R, 1:1000, Bioss, Beijing, China), GAPDH (10494-1-AP, 1:5000, Proteintech, Wuhan, China).

    Techniques: Western Blot, Expressing, Control, TUNEL Assay, Staining, Membrane, Flow Cytometry, Immunofluorescence