anti programmed death ligand 1  (Bioss)

Journal: Frontiers in Pharmacology

Article Title: Calf Thymus Polypeptide Restrains the Growth of Colorectal Tumor via Regulating the Intestinal Microbiota-Mediated Immune Function

doi: 10.3389/fphar.2022.898906

Figure Lengend Snippet: CTP regulated the expression of proteins associated with immunity in the spleen and colorectal tumors of Apc Min/+ mice. CTP regulated the expression levels of CD4, CD8, CTLA4, PD-L1, and IL-2 in (A) the colorectal tumor and (B) spleen of Apc Min/+ mice (C) CTP regulated the expression levels of Calcineurin A, NFAT1, P-PI3K, P-AKT, P-IKKα/β, and P-NF-κB p65 in the spleen of Apc Min/+ mice. Quantification data were normalized by GAPDH and their corresponding total proteins were reported as fold change with respect to the expression data from the corresponding vehicle-treated mice ( n = 4).

Article Snippet: The membranes were blocked with 5% bovine serum albumin (Gen-view Scientific, Galveston, TX, United States ) at 4°C for 4 h and then incubated with primary antibodies including CD4 (bs-0647R, 1:1000 dilution), CD8 (bs-0648R, 1:1000 dilution), PD-1 (bs-1867R, 1:1000 dilution), PD-L1 (bs-4941R, 1:1000 dilution), cytotoxic T lymphocyte associated antigen 4 (CTLA4) (bs-10006R, 1:1000 dilution) (Bioss, Beijing, China), nuclear factor of activated T cells 1 (NFAT1) (ab49161, 1:800 dilution), calcineurin A (ab109412, 1:10000 dilution), protein kinase B (AKT) (ab200195, 1:2000 dilution), phosphor (P)-AKT (ab108266, 1:2000 dilution) (Abcam, Cambridge, MA, United States ), inhibitor of nuclear factor kappa-B kinase α/β (IKKα/β) (A2062, 1:1000 dilution), P-IKKα/β (AP0891, 1:1000 dilution), nuclear Factor κB (NF-κB) p65 (A19653, 1:1000 dilution), P-NF-κB p65 (AP0475, 1:500 dilution), IL-2 (A16317, 1:1000 dilution) (Abclonal, Wuhan, China), phosphatidylinositol 3-kinase (PI3K) (4263, 1:1000 dilution), P-PI3K (4228, 1:1000 dilution) (Cell Signaling Technology, Danvers, MA, United States ), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ABS16, 1:1000 dilution) (Millipore, Merck, Darmstadt, Germany) overnight at 4°C.

Techniques: Expressing