anti calreticulin af488 conjugated antibody  (Bioss)

Journal: Scientific Reports

Article Title: Antitumor immunostimulatory effect via cell-killing action of a novel extracorporeal blood circulating photodynamic therapy system using 5-aminolevulinic acid

doi: 10.1038/s41598-024-84861-8

Figure Lengend Snippet: 5-ALA/PDT-induced cell death and calreticulin expression. (A and B) Flow cytometric analysis of 5-ALA/PDT-treated cells stained with Annexin V and propidium iodide (PI) at low ( a ) and high irradiation ( b ). ( c ) Summary of cell death immediately (0 h) and 4 and 24 h after low or high irradiation. ( d ) Flow cytometric analysis of cell surface calreticulin at 0, 4, and 24 h after low or high irradiation. ( e ) Average of mean fluorescence intensity (MFI) of calreticulin. Values are presented as mean (SD) **p < 0.01, low irradiation versus high irradiation. N.T.: Not statistical tested because of n = 2.

Article Snippet: Cell surface calreticulin expression was assessed using FITC-conjugated anti-calreticulin polyclonal antibody (Bioss).

Techniques: Expressing, Staining, Irradiation, Fluorescence

Journal: Scientific Reports

Article Title: Antitumor immunostimulatory effect via cell-killing action of a novel extracorporeal blood circulating photodynamic therapy system using 5-aminolevulinic acid

doi: 10.1038/s41598-024-84861-8

Figure Lengend Snippet: Antitumor immunostimulatory effect of 5-ALA/PDT-treated cells or anticancer drug–treated cells. ( a ) Changes in tumor volume after allogenic C6 cell transplantation in rats with prior intravenous administration of PBS (control), 5-ALA/PDT with low irradiation–, mitoxantrone (MTX)-, doxorubicin (DOX)-, or epirubicin (EPI)-treated C6 cells (n = 3 per group). **p < 0.01 compared with the control group. ( b ) Flow cytometric analysis of low-irradiated 5-ALA/PDT–, MTX-, DOX-, or EPI-treated cells stained with Annexin V and PI. ( c ) Summary of cell death after each treatment. ( d ) Flow cytometric analysis of cell surface calreticulin of low-irradiated 5-ALA/PDT–, MTX-, DOX-, or EPI-treated cells. ( e ) Average MFI of calreticulin after each treatment. Values are presented as mean (SD) **p < 0.01, comparison with immediately (0 h) after low irradiation 5-ALA/PDT.

Article Snippet: Cell surface calreticulin expression was assessed using FITC-conjugated anti-calreticulin polyclonal antibody (Bioss).

Techniques: Transplantation Assay, Control, Irradiation, Staining, Comparison