cd86 bs 2508r mab  (Bioss)

Journal: Frontiers in Pharmacology

Article Title: Astrocyte Function Is Affected by Aging and Not Alzheimer’s Disease: A Preliminary Investigation in Hippocampi of 3xTg-AD Mice

doi: 10.3389/fphar.2019.00644

Figure Lengend Snippet: Effects of aging on hippocampal microglia of 3×Tg-AD and Non-Tg mice. (A) Representative Western blots for Iba1 and CD11b/c and (B , C) densitometric analyses normalized to β-actin loading control. Results are expressed as means ± SEM of percentage of controls (6-month-old/Non-Tg) (N = 3, in triplicate). Statistical analysis was performed by two-way ANOVA followed by Bonferroni’s post hoc test ( # p < 0.05, 6-month-old/3×Tg-AD vs 6-month-old/Non-Tg).

Article Snippet: Membranes were then incubated overnight with one of the following primary antibodies: rabbit anti-GFAP (1:25,000; Abcam, UK), rabbit anti-S100B (1:1,000; Novus Biological, CO), rabbit anti-Iba1 (1:1,000; Abcam), rabbit anti-CD11b/c (1:1,000; Bioss, MA), mouse anti-CX43 (1:500; EMD Millipore, MA), mouse anti-AQP4 (1:500; Santa Cruz, TX), rabbit anti-BDNF (1:1,000; Abcam), mouse anti-β-amyloid (1:200; Millipore, Germany), rabbit anti-p[Ser396]tau (1:1,000; Thermo Fisher Scientific, MA).

Techniques: Western Blot

Journal: Oncology Reports

Article Title: PARG regulates the proliferation and differentiation of DCs and T cells via PARP/NF-κB in tumour metastases of colon carcinoma

doi: 10.3892/or.2019.7051

Figure Lengend Snippet: CD11c + CD11b + DCs in the spleen and liver of various groups (×200). (A) CD11c antigen expressed on the cell membrane as green fluorescence with FITC staining, CD11b antigen expressed on the cell membrane as emerged red fluorescence with Cy3 staining, CD11c and CD11b antigens co-expressed on the membrane as yellow fluorescence with FITC and Cy3 dual staining. (B) CD11c antigen expressed on the cell membrane as green fluorescence with FITC staining, CD11b antigen expressed on the cell membrane as red fluorescence with Cy3 staining, CD11c and CD11b antigens co-expressed on the membrane as yellow fluorescence with FITC and Cy3 dual staining. (C) Number of CD11c + CD11b + DCs in the spleen and liver of various groups, with a significant increase in the PARG-silenced group compared with that in the non-transfected or transfected with empty vector group. *P<0.05 vs. control groups. The silenced PARG in CT26 cells could promote the distribution of B220 + DEC205 + DCs in CT26 liver metastases and CT26 spleen inoculated tumours. DC, dendritic cell; FITC, fluorescein isothiocyanate; Cy3, cyanine 3; PARG, poly(ADP-ribose) glycohydrolase.

Article Snippet: The spleen or liver sections were first incubated with anti-DEC205 antibody (cat. no. ab51820; 1:50 diluted in PBS; Abcam, Cambridge, MA, USA) or anti-CD11b antibody (cat. no. sc-1186; 1:50 diluted in PBS; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), followed by the addition of anti-B220 antibody (cat. no. bs-10599R; 1:100 diluted in PBS; BIOSS, Beijing, China) or anti-CD11c antibody (cat. no. bs-2508R; 1:50 diluted in PBS; BIOSS) at 4°C overnight.

Techniques: Fluorescence, Staining, Transfection, Plasmid Preparation