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anti kif5b  (Bioss)


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    Structured Review

    Bioss anti kif5b
    Primer sequences
    Anti Kif5b, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kif5b/product/Bioss
    Average 94 stars, based on 1 article reviews
    anti kif5b - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis"

    Article Title: LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

    Journal: Oncology Research

    doi: 10.32604/or.2024.047454

    Primer sequences
    Figure Legend Snippet: Primer sequences

    Techniques Used:

    PCGEM1 regulated KIF5B expression by competing for miR-642a-5p. (A) The mRNA expression of miR-642a-5p was measured in Hela and SiHa cells transfected with sh-PCGEM1 or sh-NC. ** p < 0.01 vs . sh-NC group. (B) The conceptual representation of the miR-642a-5p combining sites in PCGEM1. (C) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT miR-642a-5p were co-transfected with PCGEM1 mimic or mimic NC. * p < 0.05 vs . Mimics NC group. (D–E) In Hela and SiHa cells transfected with miR-642a-5p mimic or mimic NC, the mRNA and protein levels of KIF5B were assessed. * p < 0.05 vs . Mimics NC group. (F) The miR-642a-5p putative targeting sites in the WT and MUT of KIF5B are shown schematically. (G) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT KIF5B were co-transfected with miR-642a-5p mimic or mimic NC. * p < 0.05 vs . Mimics NC group.
    Figure Legend Snippet: PCGEM1 regulated KIF5B expression by competing for miR-642a-5p. (A) The mRNA expression of miR-642a-5p was measured in Hela and SiHa cells transfected with sh-PCGEM1 or sh-NC. ** p < 0.01 vs . sh-NC group. (B) The conceptual representation of the miR-642a-5p combining sites in PCGEM1. (C) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT miR-642a-5p were co-transfected with PCGEM1 mimic or mimic NC. * p < 0.05 vs . Mimics NC group. (D–E) In Hela and SiHa cells transfected with miR-642a-5p mimic or mimic NC, the mRNA and protein levels of KIF5B were assessed. * p < 0.05 vs . Mimics NC group. (F) The miR-642a-5p putative targeting sites in the WT and MUT of KIF5B are shown schematically. (G) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT KIF5B were co-transfected with miR-642a-5p mimic or mimic NC. * p < 0.05 vs . Mimics NC group.

    Techniques Used: Expressing, Transfection, Luciferase

    PCGEM1 silencing inhibited proliferation, metastasis, invasion, and EMT in CC cells by the regulation of miR-642a-5p/KIF5B axis. (A and B) The mRNA and protein levels of KIF5B, (C) CCK-8 assay and colony formation assay, (D) formation of colonies assays, (E) wound-healing assay (scale bar, 100 µm), (F) Transwell assay (scale bar, 50 µm), and (G) the protein levels of N-cadherin and E-cadherin were determined in SiHa cells transfected with sh-NC or sh-PCGEM1 alone, or together with miR-642a-5p inhibitor or inhibitor NC. ** p < 0.01 vs . sh-NC group, # p < 0.01 vs . sh-PCGEM1+miR-642a-5p NC group.
    Figure Legend Snippet: PCGEM1 silencing inhibited proliferation, metastasis, invasion, and EMT in CC cells by the regulation of miR-642a-5p/KIF5B axis. (A and B) The mRNA and protein levels of KIF5B, (C) CCK-8 assay and colony formation assay, (D) formation of colonies assays, (E) wound-healing assay (scale bar, 100 µm), (F) Transwell assay (scale bar, 50 µm), and (G) the protein levels of N-cadherin and E-cadherin were determined in SiHa cells transfected with sh-NC or sh-PCGEM1 alone, or together with miR-642a-5p inhibitor or inhibitor NC. ** p < 0.01 vs . sh-NC group, # p < 0.01 vs . sh-PCGEM1+miR-642a-5p NC group.

    Techniques Used: CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Assay, Transfection



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    anti kif5b  (Bioss)
    94
    Bioss anti kif5b
    Primer sequences
    Anti Kif5b, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kif5b/product/Bioss
    Average 94 stars, based on 1 article reviews
    anti kif5b - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Primer sequences

    Journal: Oncology Research

    Article Title: LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

    doi: 10.32604/or.2024.047454

    Figure Lengend Snippet: Primer sequences

    Article Snippet: Anti-KIF5B (1:2,000, bs-24715R, Bioss) and anti-GAPDH (1:2,000, bs-0755R, Bioss) antibodies are used, with GAPDH serving as the endogenous control.

    Techniques:

    PCGEM1 regulated KIF5B expression by competing for miR-642a-5p. (A) The mRNA expression of miR-642a-5p was measured in Hela and SiHa cells transfected with sh-PCGEM1 or sh-NC. ** p < 0.01 vs . sh-NC group. (B) The conceptual representation of the miR-642a-5p combining sites in PCGEM1. (C) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT miR-642a-5p were co-transfected with PCGEM1 mimic or mimic NC. * p < 0.05 vs . Mimics NC group. (D–E) In Hela and SiHa cells transfected with miR-642a-5p mimic or mimic NC, the mRNA and protein levels of KIF5B were assessed. * p < 0.05 vs . Mimics NC group. (F) The miR-642a-5p putative targeting sites in the WT and MUT of KIF5B are shown schematically. (G) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT KIF5B were co-transfected with miR-642a-5p mimic or mimic NC. * p < 0.05 vs . Mimics NC group.

    Journal: Oncology Research

    Article Title: LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

    doi: 10.32604/or.2024.047454

    Figure Lengend Snippet: PCGEM1 regulated KIF5B expression by competing for miR-642a-5p. (A) The mRNA expression of miR-642a-5p was measured in Hela and SiHa cells transfected with sh-PCGEM1 or sh-NC. ** p < 0.01 vs . sh-NC group. (B) The conceptual representation of the miR-642a-5p combining sites in PCGEM1. (C) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT miR-642a-5p were co-transfected with PCGEM1 mimic or mimic NC. * p < 0.05 vs . Mimics NC group. (D–E) In Hela and SiHa cells transfected with miR-642a-5p mimic or mimic NC, the mRNA and protein levels of KIF5B were assessed. * p < 0.05 vs . Mimics NC group. (F) The miR-642a-5p putative targeting sites in the WT and MUT of KIF5B are shown schematically. (G) In Hela and SiHa cells, the luciferase reporters harboring WT or MUT KIF5B were co-transfected with miR-642a-5p mimic or mimic NC. * p < 0.05 vs . Mimics NC group.

    Article Snippet: Anti-KIF5B (1:2,000, bs-24715R, Bioss) and anti-GAPDH (1:2,000, bs-0755R, Bioss) antibodies are used, with GAPDH serving as the endogenous control.

    Techniques: Expressing, Transfection, Luciferase

    PCGEM1 silencing inhibited proliferation, metastasis, invasion, and EMT in CC cells by the regulation of miR-642a-5p/KIF5B axis. (A and B) The mRNA and protein levels of KIF5B, (C) CCK-8 assay and colony formation assay, (D) formation of colonies assays, (E) wound-healing assay (scale bar, 100 µm), (F) Transwell assay (scale bar, 50 µm), and (G) the protein levels of N-cadherin and E-cadherin were determined in SiHa cells transfected with sh-NC or sh-PCGEM1 alone, or together with miR-642a-5p inhibitor or inhibitor NC. ** p < 0.01 vs . sh-NC group, # p < 0.01 vs . sh-PCGEM1+miR-642a-5p NC group.

    Journal: Oncology Research

    Article Title: LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

    doi: 10.32604/or.2024.047454

    Figure Lengend Snippet: PCGEM1 silencing inhibited proliferation, metastasis, invasion, and EMT in CC cells by the regulation of miR-642a-5p/KIF5B axis. (A and B) The mRNA and protein levels of KIF5B, (C) CCK-8 assay and colony formation assay, (D) formation of colonies assays, (E) wound-healing assay (scale bar, 100 µm), (F) Transwell assay (scale bar, 50 µm), and (G) the protein levels of N-cadherin and E-cadherin were determined in SiHa cells transfected with sh-NC or sh-PCGEM1 alone, or together with miR-642a-5p inhibitor or inhibitor NC. ** p < 0.01 vs . sh-NC group, # p < 0.01 vs . sh-PCGEM1+miR-642a-5p NC group.

    Article Snippet: Anti-KIF5B (1:2,000, bs-24715R, Bioss) and anti-GAPDH (1:2,000, bs-0755R, Bioss) antibodies are used, with GAPDH serving as the endogenous control.

    Techniques: CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Assay, Transfection