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rabbit anti integrin α 5 (Bioss)

Name: rabbit anti integrin α 5
Brand: Bioss
Rating: 92 (2 reviews)

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Bioss rabbit anti integrin α 5

VEGF induces angiogenesis and high <t>integrin</t> expression in differentiated MSCs. (a) DiI-Ac-LDL uptake assay in differentiated MSCs. Parallel experiments in HUVECs served as a positive control. (b) Percentages of MSCs that incorporated DiI-Ac-LDL were calculated, and the data were expressed as percentages compared with the overall cell count. ∗∗ P < 0.01, n = 3. (c) MSCs seeded onto a basement membrane-like gel were treated with VEGF for 4, 7, and 14 days. VEGF-free MSCs were seeded onto a basement membrane-like gel for 7 days (Control group). (d) Capillary-like structures were quantified by measuring the polygonal network. (e) After MSCs were cultured in EC differentiation medium for 7 d, integrins α 1, α 5, and β 1 were examined by qPCR. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (f) Western blotting assay for integrins α 1, α 5, and β 1. (g) The relative quantification of the protein expression was statistically analyzed using Image J software. ∗ P < 0.05; ∗∗ P < 0.01, n = 3.

Rabbit Anti Integrin α 5, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti integrin α 5 - by Bioz Stars, 2026-02

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1) Product Images from "Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells"

Article Title: Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

Journal: Stem Cells International

doi: 10.1155/2015/534758

VEGF induces angiogenesis and high integrin expression in differentiated MSCs. (a) DiI-Ac-LDL uptake assay in differentiated MSCs. Parallel experiments in HUVECs served as a positive control. (b) Percentages of MSCs that incorporated DiI-Ac-LDL were calculated, and the data were expressed as percentages compared with the overall cell count. ∗∗ P < 0.01, n = 3. (c) MSCs seeded onto a basement membrane-like gel were treated with VEGF for 4, 7, and 14 days. VEGF-free MSCs were seeded onto a basement membrane-like gel for 7 days (Control group). (d) Capillary-like structures were quantified by measuring the polygonal network. (e) After MSCs were cultured in EC differentiation medium for 7 d, integrins α 1, α 5, and β 1 were examined by qPCR. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (f) Western blotting assay for integrins α 1, α 5, and β 1. (g) The relative quantification of the protein expression was statistically analyzed using Image J software. ∗ P < 0.05; ∗∗ P < 0.01, n = 3.

Figure Legend Snippet: VEGF induces angiogenesis and high integrin expression in differentiated MSCs. (a) DiI-Ac-LDL uptake assay in differentiated MSCs. Parallel experiments in HUVECs served as a positive control. (b) Percentages of MSCs that incorporated DiI-Ac-LDL were calculated, and the data were expressed as percentages compared with the overall cell count. ∗∗ P < 0.01, n = 3. (c) MSCs seeded onto a basement membrane-like gel were treated with VEGF for 4, 7, and 14 days. VEGF-free MSCs were seeded onto a basement membrane-like gel for 7 days (Control group). (d) Capillary-like structures were quantified by measuring the polygonal network. (e) After MSCs were cultured in EC differentiation medium for 7 d, integrins α 1, α 5, and β 1 were examined by qPCR. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (f) Western blotting assay for integrins α 1, α 5, and β 1. (g) The relative quantification of the protein expression was statistically analyzed using Image J software. ∗ P < 0.05; ∗∗ P < 0.01, n = 3.

Techniques Used: Expressing, Positive Control, Cell Counting, Cell Culture, Western Blot, Software

MRTF-A is essential for the VEGF-induced angiogenesis of differentiated MSCs and integrin α 1, α 5, and β 1 expression. (a) After transfection with sh-MRTF-A or pSUPER (control), MSCs were cultured with EC differentiation medium for 3 d. MTT assay was performed to test the viability of MSCs. (b and c) MSCs were differentiated into ECs by treatment with VEGF for 7 d and siRNA-mediated knockdown experiments were then performed in the MSCs-derived ECs. The migratory ability of MSC-derived ECs transfected with si-MRTF-A or control siRNA was determined via wound healing (b) and transwell chamber assays (c). (d) Cell migration was quantified by calculating relative cell numbers. ∗∗ P < 0.01, n = 3. (e) After differentiation and siRNA or shRNA-mediated knockdown, the cells were cultured in matrigel for 7 d. Morphological changes in differentiated cells transfected with siMRTF-A or sh-MRTF-A were observed. (f) Capillary-like structures were quantified by measuring the polygonal network. ∗∗ P < 0.01, n = 3. (g) The expression of integrins α 1, α 5, and β 1 was estimated by qPCR. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (h) The expression of integrins α 1, α 5 and β 1 was estimated by western blot. (i) The relative quantification of the protein expression. ∗ P < 0.05; ∗∗ P < 0.01, n = 3.

Figure Legend Snippet: MRTF-A is essential for the VEGF-induced angiogenesis of differentiated MSCs and integrin α 1, α 5, and β 1 expression. (a) After transfection with sh-MRTF-A or pSUPER (control), MSCs were cultured with EC differentiation medium for 3 d. MTT assay was performed to test the viability of MSCs. (b and c) MSCs were differentiated into ECs by treatment with VEGF for 7 d and siRNA-mediated knockdown experiments were then performed in the MSCs-derived ECs. The migratory ability of MSC-derived ECs transfected with si-MRTF-A or control siRNA was determined via wound healing (b) and transwell chamber assays (c). (d) Cell migration was quantified by calculating relative cell numbers. ∗∗ P < 0.01, n = 3. (e) After differentiation and siRNA or shRNA-mediated knockdown, the cells were cultured in matrigel for 7 d. Morphological changes in differentiated cells transfected with siMRTF-A or sh-MRTF-A were observed. (f) Capillary-like structures were quantified by measuring the polygonal network. ∗∗ P < 0.01, n = 3. (g) The expression of integrins α 1, α 5, and β 1 was estimated by qPCR. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (h) The expression of integrins α 1, α 5 and β 1 was estimated by western blot. (i) The relative quantification of the protein expression. ∗ P < 0.05; ∗∗ P < 0.01, n = 3.

Techniques Used: Expressing, Transfection, Cell Culture, MTT Assay, Derivative Assay, Migration, shRNA, Western Blot

MRTF-A activates the transcription of integrins α 1 and α 5 by binding to the CArG box with their promoters. (a and b) Luciferase assays were performed 24 h after transfection of MRTF-A and integrin α 1/integrin α 5 promoter-luc plasmids into MSCs, COS-7 cells and HUVECs. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (c and d) ChIP assays were performed in MSCs treated with VEGF at d2, d4, and d7. Cross-linked chromatin was immunoprecipitated with specific anti-MRTF-A antibody. The precipitated chromatin DNA was then purified and amplified by reverse transcription PCR (c) and real time PCR (d) with specific primers that spanned CArG boxes in integrin promoters. The negative control in the immune-precipitation was performed with IgG antibody.

Figure Legend Snippet: MRTF-A activates the transcription of integrins α 1 and α 5 by binding to the CArG box with their promoters. (a and b) Luciferase assays were performed 24 h after transfection of MRTF-A and integrin α 1/integrin α 5 promoter-luc plasmids into MSCs, COS-7 cells and HUVECs. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (c and d) ChIP assays were performed in MSCs treated with VEGF at d2, d4, and d7. Cross-linked chromatin was immunoprecipitated with specific anti-MRTF-A antibody. The precipitated chromatin DNA was then purified and amplified by reverse transcription PCR (c) and real time PCR (d) with specific primers that spanned CArG boxes in integrin promoters. The negative control in the immune-precipitation was performed with IgG antibody.

Techniques Used: Binding Assay, Luciferase, Transfection, Immunoprecipitation, Purification, Amplification, Real-time Polymerase Chain Reaction, Negative Control

VEGF induces angiogenesis and high integrin expression in differentiated MSCs via the Rho signaling pathway. (a) MSCs were cultured with EC differentiation medium in the presence or absence of the Rho inhibitor C3 transferase or the Rho-associated protein kinase (ROCK) inhibitor Y27632 for 4 d and then MTT assay was performed to test cellular viability. (b) MSCs were pretreated with either the C3 or Y27632 in the present of VEGF for 4 d and then continuously cultured in EC differentiated medium for 3 d. Matrigel angiogenesis assays were then performed. (c) Capillary-like structures were quantified by measuring the polygonal network. (d) The expression of integrins α 1, α 5 and β 1 during endothelial differentiation of MSCs was estimated by real-time PCR. ∗∗ P < 0.01, n = 3. (e) Western blotting assay for integrins α 1, α 5 and β 1. (f) The relative quantification of the protein expression. ∗∗ P < 0.01, n = 3.

Figure Legend Snippet: VEGF induces angiogenesis and high integrin expression in differentiated MSCs via the Rho signaling pathway. (a) MSCs were cultured with EC differentiation medium in the presence or absence of the Rho inhibitor C3 transferase or the Rho-associated protein kinase (ROCK) inhibitor Y27632 for 4 d and then MTT assay was performed to test cellular viability. (b) MSCs were pretreated with either the C3 or Y27632 in the present of VEGF for 4 d and then continuously cultured in EC differentiated medium for 3 d. Matrigel angiogenesis assays were then performed. (c) Capillary-like structures were quantified by measuring the polygonal network. (d) The expression of integrins α 1, α 5 and β 1 during endothelial differentiation of MSCs was estimated by real-time PCR. ∗∗ P < 0.01, n = 3. (e) Western blotting assay for integrins α 1, α 5 and β 1. (f) The relative quantification of the protein expression. ∗∗ P < 0.01, n = 3.

Techniques Used: Expressing, Cell Culture, MTT Assay, Real-time Polymerase Chain Reaction, Western Blot

Knocking-down MRTF-A obstructed angiogenesis and migration in HUVECs by affecting integrin expression. (a) Western blotting confirmed the inhibition of endogenous MRTF-A in HUVECs transfected with siMRTF-A. (b) The capillary-like structures formed by HUVECs were observed on Matrigel. (c) Quantification of the capillary-like structures. ∗∗ P < 0.01, n = 3. The migratory ability of HUVECs transfected with siMRTF-A or control siRNA was determined by wound healing (d) and transwell chamber assays (e). (f) Cell migration was quantified by calculating relative cell numbers. ∗ P < 0.05, n = 3. (g) After transfection with siMRTF-A or control siRNA, HUVECs were treated with VEGF for 24 h, and the expression of integrins α 1, α 5, and β 1 was then estimated by real-time PCR. ∗∗ P < 0.01, n = 3. (h) Western blotting for integrins α 1, α 5 and β 1. (i) The relative quantification of the protein expression. ∗∗ P < 0.01, n = 3.

Figure Legend Snippet: Knocking-down MRTF-A obstructed angiogenesis and migration in HUVECs by affecting integrin expression. (a) Western blotting confirmed the inhibition of endogenous MRTF-A in HUVECs transfected with siMRTF-A. (b) The capillary-like structures formed by HUVECs were observed on Matrigel. (c) Quantification of the capillary-like structures. ∗∗ P < 0.01, n = 3. The migratory ability of HUVECs transfected with siMRTF-A or control siRNA was determined by wound healing (d) and transwell chamber assays (e). (f) Cell migration was quantified by calculating relative cell numbers. ∗ P < 0.05, n = 3. (g) After transfection with siMRTF-A or control siRNA, HUVECs were treated with VEGF for 24 h, and the expression of integrins α 1, α 5, and β 1 was then estimated by real-time PCR. ∗∗ P < 0.01, n = 3. (h) Western blotting for integrins α 1, α 5 and β 1. (i) The relative quantification of the protein expression. ∗∗ P < 0.01, n = 3.

Techniques Used: Migration, Expressing, Western Blot, Inhibition, Transfection, Real-time Polymerase Chain Reaction

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Journal: Stem Cells International

Article Title: Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

doi: 10.1155/2015/534758

Figure Lengend Snippet: VEGF induces angiogenesis and high integrin expression in differentiated MSCs. (a) DiI-Ac-LDL uptake assay in differentiated MSCs. Parallel experiments in HUVECs served as a positive control. (b) Percentages of MSCs that incorporated DiI-Ac-LDL were calculated, and the data were expressed as percentages compared with the overall cell count. ∗∗ P < 0.01, n = 3. (c) MSCs seeded onto a basement membrane-like gel were treated with VEGF for 4, 7, and 14 days. VEGF-free MSCs were seeded onto a basement membrane-like gel for 7 days (Control group). (d) Capillary-like structures were quantified by measuring the polygonal network. (e) After MSCs were cultured in EC differentiation medium for 7 d, integrins α 1, α 5, and β 1 were examined by qPCR. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (f) Western blotting assay for integrins α 1, α 5, and β 1. (g) The relative quantification of the protein expression was statistically analyzed using Image J software. ∗ P < 0.05; ∗∗ P < 0.01, n = 3.

Article Snippet: The following primary antibodies were used: rabbit anti-integrin α 5 (Bioss), anti-integrin α 1 (Bioss), anti-integrin β 1 (Abcam), and mouse anti-GAPDH (Santa Cruz).

Techniques: Expressing, Positive Control, Cell Counting, Cell Culture, Western Blot, Software

Journal: Stem Cells International

Article Title: Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

doi: 10.1155/2015/534758

Figure Lengend Snippet: MRTF-A is essential for the VEGF-induced angiogenesis of differentiated MSCs and integrin α 1, α 5, and β 1 expression. (a) After transfection with sh-MRTF-A or pSUPER (control), MSCs were cultured with EC differentiation medium for 3 d. MTT assay was performed to test the viability of MSCs. (b and c) MSCs were differentiated into ECs by treatment with VEGF for 7 d and siRNA-mediated knockdown experiments were then performed in the MSCs-derived ECs. The migratory ability of MSC-derived ECs transfected with si-MRTF-A or control siRNA was determined via wound healing (b) and transwell chamber assays (c). (d) Cell migration was quantified by calculating relative cell numbers. ∗∗ P < 0.01, n = 3. (e) After differentiation and siRNA or shRNA-mediated knockdown, the cells were cultured in matrigel for 7 d. Morphological changes in differentiated cells transfected with siMRTF-A or sh-MRTF-A were observed. (f) Capillary-like structures were quantified by measuring the polygonal network. ∗∗ P < 0.01, n = 3. (g) The expression of integrins α 1, α 5, and β 1 was estimated by qPCR. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (h) The expression of integrins α 1, α 5 and β 1 was estimated by western blot. (i) The relative quantification of the protein expression. ∗ P < 0.05; ∗∗ P < 0.01, n = 3.

Article Snippet: The following primary antibodies were used: rabbit anti-integrin α 5 (Bioss), anti-integrin α 1 (Bioss), anti-integrin β 1 (Abcam), and mouse anti-GAPDH (Santa Cruz).

Techniques: Expressing, Transfection, Cell Culture, MTT Assay, Derivative Assay, Migration, shRNA, Western Blot

Journal: Stem Cells International

Article Title: Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

doi: 10.1155/2015/534758

Figure Lengend Snippet: MRTF-A activates the transcription of integrins α 1 and α 5 by binding to the CArG box with their promoters. (a and b) Luciferase assays were performed 24 h after transfection of MRTF-A and integrin α 1/integrin α 5 promoter-luc plasmids into MSCs, COS-7 cells and HUVECs. ∗ P < 0.05; ∗∗ P < 0.01, n = 3. (c and d) ChIP assays were performed in MSCs treated with VEGF at d2, d4, and d7. Cross-linked chromatin was immunoprecipitated with specific anti-MRTF-A antibody. The precipitated chromatin DNA was then purified and amplified by reverse transcription PCR (c) and real time PCR (d) with specific primers that spanned CArG boxes in integrin promoters. The negative control in the immune-precipitation was performed with IgG antibody.

Article Snippet: The following primary antibodies were used: rabbit anti-integrin α 5 (Bioss), anti-integrin α 1 (Bioss), anti-integrin β 1 (Abcam), and mouse anti-GAPDH (Santa Cruz).

Techniques: Binding Assay, Luciferase, Transfection, Immunoprecipitation, Purification, Amplification, Real-time Polymerase Chain Reaction, Negative Control

Journal: Stem Cells International

Article Title: Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

doi: 10.1155/2015/534758

Figure Lengend Snippet: VEGF induces angiogenesis and high integrin expression in differentiated MSCs via the Rho signaling pathway. (a) MSCs were cultured with EC differentiation medium in the presence or absence of the Rho inhibitor C3 transferase or the Rho-associated protein kinase (ROCK) inhibitor Y27632 for 4 d and then MTT assay was performed to test cellular viability. (b) MSCs were pretreated with either the C3 or Y27632 in the present of VEGF for 4 d and then continuously cultured in EC differentiated medium for 3 d. Matrigel angiogenesis assays were then performed. (c) Capillary-like structures were quantified by measuring the polygonal network. (d) The expression of integrins α 1, α 5 and β 1 during endothelial differentiation of MSCs was estimated by real-time PCR. ∗∗ P < 0.01, n = 3. (e) Western blotting assay for integrins α 1, α 5 and β 1. (f) The relative quantification of the protein expression. ∗∗ P < 0.01, n = 3.

Article Snippet: The following primary antibodies were used: rabbit anti-integrin α 5 (Bioss), anti-integrin α 1 (Bioss), anti-integrin β 1 (Abcam), and mouse anti-GAPDH (Santa Cruz).

Techniques: Expressing, Cell Culture, MTT Assay, Real-time Polymerase Chain Reaction, Western Blot

Journal: Stem Cells International

Article Title: Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

doi: 10.1155/2015/534758

Figure Lengend Snippet: Knocking-down MRTF-A obstructed angiogenesis and migration in HUVECs by affecting integrin expression. (a) Western blotting confirmed the inhibition of endogenous MRTF-A in HUVECs transfected with siMRTF-A. (b) The capillary-like structures formed by HUVECs were observed on Matrigel. (c) Quantification of the capillary-like structures. ∗∗ P < 0.01, n = 3. The migratory ability of HUVECs transfected with siMRTF-A or control siRNA was determined by wound healing (d) and transwell chamber assays (e). (f) Cell migration was quantified by calculating relative cell numbers. ∗ P < 0.05, n = 3. (g) After transfection with siMRTF-A or control siRNA, HUVECs were treated with VEGF for 24 h, and the expression of integrins α 1, α 5, and β 1 was then estimated by real-time PCR. ∗∗ P < 0.01, n = 3. (h) Western blotting for integrins α 1, α 5 and β 1. (i) The relative quantification of the protein expression. ∗∗ P < 0.01, n = 3.

Article Snippet: The following primary antibodies were used: rabbit anti-integrin α 5 (Bioss), anti-integrin α 1 (Bioss), anti-integrin β 1 (Abcam), and mouse anti-GAPDH (Santa Cruz).

Techniques: Migration, Expressing, Western Blot, Inhibition, Transfection, Real-time Polymerase Chain Reaction

α 5 integrin and phalloidin staining delineate the distal tips of the arrector pili muscle. Immunofluorescence microscopy showing patterns of phalloidin, expression of α 5 integrin, and collagen VII. (a-b) α 5 integrin (red) demonstrates a heterogeneous expression in the basal epidermis. (b) Triple immunofluorescence staining for α 5 integrin (red), collagen VII (green), and phalloidin (blue) showing marked overlap of α 5 integrin and phalloidin in the basal epidermis at the distal tip of the arrector pili muscle. (c) Strong correlation between α 5 integrin expressing cells and cells stained with phalloidin. (d) Extensive branching of the APM (phalloidin-stained) as it ascends and attaches to the dermoepidermal junction. Scale bar: 100 μ m.

Journal: Stem Cells International

Article Title: Epidermal Cells Expressing Putative Cell Markers in Nonglabrous Skin Existing in Direct Proximity with the Distal End of the Arrector Pili Muscle

doi: 10.1155/2016/1286315

Figure Lengend Snippet: α 5 integrin and phalloidin staining delineate the distal tips of the arrector pili muscle. Immunofluorescence microscopy showing patterns of phalloidin, expression of α 5 integrin, and collagen VII. (a-b) α 5 integrin (red) demonstrates a heterogeneous expression in the basal epidermis. (b) Triple immunofluorescence staining for α 5 integrin (red), collagen VII (green), and phalloidin (blue) showing marked overlap of α 5 integrin and phalloidin in the basal epidermis at the distal tip of the arrector pili muscle. (c) Strong correlation between α 5 integrin expressing cells and cells stained with phalloidin. (d) Extensive branching of the APM (phalloidin-stained) as it ascends and attaches to the dermoepidermal junction. Scale bar: 100 μ m.

Article Snippet: The rabbit polyclonal antibodies against α 6 and α 5 integrin (Bioss Antibodies, Woburn, MA) were diluted to 1 : 50.

Techniques: Staining, Immunofluorescence, Microscopy, Expressing

Localization of K15 in the interfollicular epidermis and follicle. Immunofluorescence microscopy showing patterns of phalloidin, expression of α 5 integrin, K15, and collagen VII. (a) K15 (red) demonstrates a heterogeneous expression in the basal epidermis and follicular bulge. K15 expression is evident at the distal attachment of the APM (green) in the epidermis. (b) Magnified view of the distal attachment of the APM. K15 (red) expression at the distal APM (green) attachment. (c) Triple immunofluorescence staining for K15 (red), collagen VII (green), and phalloidin (blue) showing K15 expressing cells and cells stained with phalloidin in the basal epidermis at the distal tip of the arrector pili muscle (blue) 100 μ m.

Journal: Stem Cells International

Article Title: Epidermal Cells Expressing Putative Cell Markers in Nonglabrous Skin Existing in Direct Proximity with the Distal End of the Arrector Pili Muscle

doi: 10.1155/2016/1286315

Figure Lengend Snippet: Localization of K15 in the interfollicular epidermis and follicle. Immunofluorescence microscopy showing patterns of phalloidin, expression of α 5 integrin, K15, and collagen VII. (a) K15 (red) demonstrates a heterogeneous expression in the basal epidermis and follicular bulge. K15 expression is evident at the distal attachment of the APM (green) in the epidermis. (b) Magnified view of the distal attachment of the APM. K15 (red) expression at the distal APM (green) attachment. (c) Triple immunofluorescence staining for K15 (red), collagen VII (green), and phalloidin (blue) showing K15 expressing cells and cells stained with phalloidin in the basal epidermis at the distal tip of the arrector pili muscle (blue) 100 μ m.

Article Snippet: The rabbit polyclonal antibodies against α 6 and α 5 integrin (Bioss Antibodies, Woburn, MA) were diluted to 1 : 50.

Techniques: Immunofluorescence, Microscopy, Expressing, Staining

(a) Triple immunofluorescence staining for K15 (red), α 5 integrin (green), and phalloidin (blue) showing K15 and phalloidin expressing cells in the basal epidermis at the distal tip of the arrector pili muscle (blue). Marked overlap (yellow) of K15 (red) and α 5 integrin (green) expressing cells in the basal epidermis. (b) Magnified view of the proximal attachment of the APM to the follicle. Expression of K15 and α 5 integrin at the bulge is evident. (c-d) Strong correlation between K15 and phalloidin and also K15 and α 5 integrin expressing cells in the epidermis. Scale bar: 100 μ m.

Journal: Stem Cells International

Article Title: Epidermal Cells Expressing Putative Cell Markers in Nonglabrous Skin Existing in Direct Proximity with the Distal End of the Arrector Pili Muscle

doi: 10.1155/2016/1286315

Figure Lengend Snippet: (a) Triple immunofluorescence staining for K15 (red), α 5 integrin (green), and phalloidin (blue) showing K15 and phalloidin expressing cells in the basal epidermis at the distal tip of the arrector pili muscle (blue). Marked overlap (yellow) of K15 (red) and α 5 integrin (green) expressing cells in the basal epidermis. (b) Magnified view of the proximal attachment of the APM to the follicle. Expression of K15 and α 5 integrin at the bulge is evident. (c-d) Strong correlation between K15 and phalloidin and also K15 and α 5 integrin expressing cells in the epidermis. Scale bar: 100 μ m.

Article Snippet: The rabbit polyclonal antibodies against α 6 and α 5 integrin (Bioss Antibodies, Woburn, MA) were diluted to 1 : 50.

Techniques: Immunofluorescence, Staining, Expressing

Localization of MCSP in the interfollicular epidermis. Immunofluorescence microscopy showing patterns of expression of MCSP, phalloidin, and K15. (a) Significant MCSP (green) and α 5 integrin (red) colocalization (yellow) was noted in the interfollicular epidermis. (b) A strong correlation between α 5 integrin and MCSP was seen suggesting that cells expressing MCSP in the basal epidermis are located in close proximity to the attachments sites of the APM. Scale bar: 100 μ m.

Journal: Stem Cells International

Article Title: Epidermal Cells Expressing Putative Cell Markers in Nonglabrous Skin Existing in Direct Proximity with the Distal End of the Arrector Pili Muscle

doi: 10.1155/2016/1286315

Figure Lengend Snippet: Localization of MCSP in the interfollicular epidermis. Immunofluorescence microscopy showing patterns of expression of MCSP, phalloidin, and K15. (a) Significant MCSP (green) and α 5 integrin (red) colocalization (yellow) was noted in the interfollicular epidermis. (b) A strong correlation between α 5 integrin and MCSP was seen suggesting that cells expressing MCSP in the basal epidermis are located in close proximity to the attachments sites of the APM. Scale bar: 100 μ m.

Article Snippet: The rabbit polyclonal antibodies against α 6 and α 5 integrin (Bioss Antibodies, Woburn, MA) were diluted to 1 : 50.

Techniques: Immunofluorescence, Microscopy, Expressing

Localization of α 6 integrin in the interfollicular epidermis and follicle. Immunofluorescence microscopy showing patterns of expression of α 5 integrin, α 6 integrin, and phalloidin. (a) α 6 integrin (red) demonstrates a homogeneous expression in the basal epidermis. (b) Triple immunofluorescence staining for α 6 integrin (red), α 5 integrin (green), and phalloidin (blue) showing overlap of α 6 integrin and α 5 integrin expressing cells at the distal tips of the APM. (c-d) Due to the homogenous expression pattern of α 6 integrin it was deemed not to be a suitable marker for IFE stem cells. Scale bar: 100 μ m.

Journal: Stem Cells International

Article Title: Epidermal Cells Expressing Putative Cell Markers in Nonglabrous Skin Existing in Direct Proximity with the Distal End of the Arrector Pili Muscle

doi: 10.1155/2016/1286315

Figure Lengend Snippet: Localization of α 6 integrin in the interfollicular epidermis and follicle. Immunofluorescence microscopy showing patterns of expression of α 5 integrin, α 6 integrin, and phalloidin. (a) α 6 integrin (red) demonstrates a homogeneous expression in the basal epidermis. (b) Triple immunofluorescence staining for α 6 integrin (red), α 5 integrin (green), and phalloidin (blue) showing overlap of α 6 integrin and α 5 integrin expressing cells at the distal tips of the APM. (c-d) Due to the homogenous expression pattern of α 6 integrin it was deemed not to be a suitable marker for IFE stem cells. Scale bar: 100 μ m.

Article Snippet: The rabbit polyclonal antibodies against α 6 and α 5 integrin (Bioss Antibodies, Woburn, MA) were diluted to 1 : 50.

Techniques: Immunofluorescence, Microscopy, Expressing, Staining, Marker

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1) Product Images from "Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells"

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