Journal: Experimental and Therapeutic Medicine
Article Title: Bisdemethoxycurcumin attenuates OVA-induced food allergy by inhibiting the MAPK and NF-κB signaling pathways
doi: 10.3892/etm.2022.11328
Figure Lengend Snippet: Effects of BDMC on the activation of MAPK and NF-κB pathway. (A and B) The expression levels of MAPK pathway related proteins p-p38, p38, p-JNK, JNK, p-ERK and ERK by western blot analysis. (C and D) The expression levels of NF-κB pathway related proteins p-NF-κBp65, NF-κBp65, IκBα and p-IκBα. The density of each band was quantified by ImageJ Software. The data are presented as the mean ± standard deviation. Data are representative of 3 independent experiments. ### P<0.001 vs. Control group; *** P<0.001 vs. OVA group. BDMC, bisdemethoxycurcumin; p-, phosphorylated; OVA, ovalbumin.
Article Snippet: Anti-p38 (cat. no. bs-33423M), anti-phosphorylated (p)-p38 (cat. no. bs-0636R), anti-JNK (cat. no. bs-0636R), anti-p-JNK (cat. no. bs-4163R), anti-ERK (cat. no. bsm-33337M), anti-p-ERK (cat. no. bs-3016R), anti-IκBα (cat. no. bs-1287R), anti-p-IκBα (cat. no. bs-5514R), anti-NF-κB p65 (cat. no. bs-23216R), anti-p-NF-κB p65 (cat. no. bs-0982R) primary antibodies were purchased from BIOSS; anti-β-actin (cat. no. AA128) primary antibody was purchased from Beyotime Institute of Biotechnology.
Techniques: Activation Assay, Expressing, Western Blot, Software, Standard Deviation