Journal: Molecular Medicine Reports
Article Title: Role of obesity and estrogen deficiency in non-alcoholic fatty liver disease: Insights from a mouse model
doi: 10.3892/mmr.2025.13629
Figure Lengend Snippet: Western blot analysis of inflammatory markers in liver. The figure shows the relative expression of NF-κβ, TNF-α and IL-1β for (A) females and (B) males in liver homogenates as indicated. Tukey plots indicate the median as the horizontal line, the top and bottom of the box show the upper and lower quartiles, maximum and minimum values are displayed as whiskers. *P<0.05; **P<0.01; ***P<0.001. SD, standard diet; HFD, high-fat diet; OVX, ovariectomized.
Article Snippet: The primary antibodies and dilutions used were TNF-α (1:1,000; BS2081R; BIOSS), IL-1β (1:500; P420B; Invitrogen, MA, USA) and NF-κβ p-65 (1:500; ab16502; Abcam).
Techniques: Western Blot, Expressing