zeb2 anti sip1  (Bioss)

Journal: iScience

Article Title: STEAP3 promotes triple-negative breast cancer growth through the FGFR1-mediated activation of PI3K/AKT/mTOR signaling

doi: 10.1016/j.isci.2025.112526

Figure Lengend Snippet: Modulating STEAP3 expression alters breast cancer cell proliferative, migratory, invasive, and EMT activity in vitro (A–M) Knockdown Effects: (A-G) The effects of STEAP3 KD on MDA-MB-231 and MDA-MB-468 cell proliferative activity were assessed through analyses of Ki67 and PCNA expression, CCK-8 assays, and colony formation assays. (H) The effects of STEAP3 KD on migration in a wound healing assay. Scale bars: 50 μm. (I–J) The effects of STEAP3 KD on invasion and migration in Transwell assays. Scale bars: 25 μm. (K–M) Effects of STEAP3 KD on EMT marker expression ( E-cadherin , N-cadherin , ZEB1 , ZEB2 ) as confirmed through RT-qPCR, Western immunoblotting, and TCGA database analyses. (N–T) Overexpression Effects: (N–P) The effects of STEAP3 OE on BT-549 cell proliferative activity were assessed through analyses of Ki67 and PCNA expression, CCK-8 assays, and colony formation assays. (Q) The effects of STEAP3 OE on migration in a wound healing assay. Scale bars: 50 μm. (R) The effects of STEAP3 OE on invasion and migration in Transwell assays. Scale bars: 25 μm. (S and T) Effects of STEAP3 OE on EMT marker levels were assessed via RT-qPCR and Western immunoblotting. Data are presented as mean ± SD (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: ZEB2/Anti-SIP1 , Bioss USA , Cat# bs-20485R; RRID: AB_11097973.

Techniques: Expressing, Activity Assay, In Vitro, Knockdown, CCK-8 Assay, Migration, Wound Healing Assay, Marker, Quantitative RT-PCR, Western Blot, Over Expression

Journal: Bioengineered

Article Title: Zinc finger E-Box binding homeobox 2 (ZEB2)-induced astrogliosis protected neuron from pyroptosis in cerebral ischemia and reperfusion injury.

doi: 10.1080/21655979.2021.2012551

Figure Lengend Snippet: Figure 1. ZEB2 promoted the activation of astrocyte after OGD/R in vitro. A: Western blot assay of ZEB2 expression in astrocyte. B: Quantification of Western blot band intensity. C: Immunofluorescence staining for GFAP (green fluorescence), ZEB2 (red fluores cence), and DAPI (blue) in astrocytes. D: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. E: Fluorescence intensity of ZEB2. E: Fluorescence intensity of GFAP. (Error bars represent mean ± SD, Magnification:400×; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Article Snippet: Cells fixed in 4% paraformaldehyde for 15 min. After fixation, cells permeabilized with 0.3% triton X-100 for 30 min, blocked by 10% goat serum for 60 min, then incubated with primary antibodies 5-bromo-2 -dexoyuridine (BrdU) (1:250, ab6326, abcam), Neuronal nuclei (NeuN) (1:1000, ab104224, abcam), glial fibrillary acidic protein (GFAP) (1:500, ab33922, abcam), ZEB2 (1:5000, bs-20,485 R, Bioss), brain-derived neurotrophic factor (BDNF) (1:500, ab108319, abcam) at 4°C overnight.

Techniques: Activation Assay, In Vitro, Western Blot, Expressing, Immunofluorescence, Staining, Fluorescence

Journal: Bioengineered

Article Title: Zinc finger E-Box binding homeobox 2 (ZEB2)-induced astrogliosis protected neuron from pyroptosis in cerebral ischemia and reperfusion injury.

doi: 10.1080/21655979.2021.2012551

Figure Lengend Snippet: Figure 2. ZEB2 promoted astrogliosis after OGD/R in vitro. A: Western blot assay of ZEB2 and GFAP expression in astrocyte. B: Quantification of ZEB2 Western blot band intensity. C: Quantification of ZEB2 Western blot band intensity. (Error bars represent mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Article Snippet: Cells fixed in 4% paraformaldehyde for 15 min. After fixation, cells permeabilized with 0.3% triton X-100 for 30 min, blocked by 10% goat serum for 60 min, then incubated with primary antibodies 5-bromo-2 -dexoyuridine (BrdU) (1:250, ab6326, abcam), Neuronal nuclei (NeuN) (1:1000, ab104224, abcam), glial fibrillary acidic protein (GFAP) (1:500, ab33922, abcam), ZEB2 (1:5000, bs-20,485 R, Bioss), brain-derived neurotrophic factor (BDNF) (1:500, ab108319, abcam) at 4°C overnight.

Techniques: In Vitro, Western Blot, Expressing

Journal: Bioengineered

Article Title: Zinc finger E-Box binding homeobox 2 (ZEB2)-induced astrogliosis protected neuron from pyroptosis in cerebral ischemia and reperfusion injury.

doi: 10.1080/21655979.2021.2012551

Figure Lengend Snippet: Figure 6. ZEB2 expression after AAV injection and MCAO/R model establishment. A. Western blot assay was used to evaluate the infection of AAV. B. Quantification of ZEB2 Western blot band intensity in each groups.

Article Snippet: Cells fixed in 4% paraformaldehyde for 15 min. After fixation, cells permeabilized with 0.3% triton X-100 for 30 min, blocked by 10% goat serum for 60 min, then incubated with primary antibodies 5-bromo-2 -dexoyuridine (BrdU) (1:250, ab6326, abcam), Neuronal nuclei (NeuN) (1:1000, ab104224, abcam), glial fibrillary acidic protein (GFAP) (1:500, ab33922, abcam), ZEB2 (1:5000, bs-20,485 R, Bioss), brain-derived neurotrophic factor (BDNF) (1:500, ab108319, abcam) at 4°C overnight.

Techniques: Expressing, Injection, Western Blot, Infection

Journal: Bioengineered

Article Title: Zinc finger E-Box binding homeobox 2 (ZEB2)-induced astrogliosis protected neuron from pyroptosis in cerebral ischemia and reperfusion injury.

doi: 10.1080/21655979.2021.2012551

Figure Lengend Snippet: Figure 7. Overexpression of ZEB2 in brain tissues of MCAO/R rats promoted neuron regeneration by alleviating pyroptosis. A: Representative track images of each group mice. B: Mean escape latency time during the orientation navigation test on 1, 3,7,14,21,28 days after MCAO/R. C: Time of the rats stay in the target quadrant on 28 days after MCAO/R. D: mNSS score on 1, 3,7,14,21,28 days after MCAO/R. E: Representative images of brain slices stained by TTC in different groups at 28 days after MCAO/R. F: Staining by Nissl showed the Nissl bodies of hippocampus and cerebral cortex. G: Staining by Luxol fast blue (LFB) showed the myelin of hippocampus and cerebral cortex. H: Western blot analysis of the expression of pyroptosis protein i.e., caspase-1, IL-1β, NLRP3, gasdermin, cleaved N-terminal GSDMD in brain tissue. I: Relative protein expression of caspase-1. J. Relative protein expression of IL-1β. K: Relative protein expression of NLRP3. L: Relative protein expression of gasdermin, M: Relative protein expression of cleaved N-terminal GSDMD. N: ELISA analysis of the expression of IL-1β in brain tissue of rats on 28 days after MCAO/R. O: ELISA analysis of the expression of IL-18 in brain tissue of rats on 28 days after MCAO/R. (Error bars represent mean ± SD, Magnification:400×; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Cells fixed in 4% paraformaldehyde for 15 min. After fixation, cells permeabilized with 0.3% triton X-100 for 30 min, blocked by 10% goat serum for 60 min, then incubated with primary antibodies 5-bromo-2 -dexoyuridine (BrdU) (1:250, ab6326, abcam), Neuronal nuclei (NeuN) (1:1000, ab104224, abcam), glial fibrillary acidic protein (GFAP) (1:500, ab33922, abcam), ZEB2 (1:5000, bs-20,485 R, Bioss), brain-derived neurotrophic factor (BDNF) (1:500, ab108319, abcam) at 4°C overnight.

Techniques: Over Expression, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay