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isx  (Bioss)


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    Structured Review

    Bioss isx
    Placenta expression of Mttp , Hnf4a and ApoB in <t>Isx</t> − /− dams at 4 and 24 h post-maternal BC administration. Real-time qPCR analysis was performed in placenta of dams 4 h (A, upper panel) and 24 h (B, upper panel) post-maternal administration of BC. Values are calculated using the 2 − ΔΔCT method and expressed as mean ± SD with individual data points overlaid; n = 1 placenta/dam, from 2 to 4 dams/group. Immunoblot and quantification of <t>placental</t> <t>MTP</t> (97 kDa) (n = 1–2 placenta/dam, from 3 dams/group) at 4 and 24 h are shown in the middle and lower panels A and B, respectively. β-Actin (42 kDa) was used as the loading control. Values are expressed as mean ± SD. Statistical analysis by t-test ; *p ≤ 0.05 vs . the corresponding vehicle-treated group at each time point.
    Isx, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isx/product/Bioss
    Average 94 stars, based on 2 article reviews
    isx - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "The intestine-specific homeobox (ISX) modulates β-carotene-dependent regulation of microsomal triglyceride transfer protein (MTP) in a tissue-specific manner"

    Article Title: The intestine-specific homeobox (ISX) modulates β-carotene-dependent regulation of microsomal triglyceride transfer protein (MTP) in a tissue-specific manner

    Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids

    doi: 10.1016/j.bbalip.2024.159584

    Placenta expression of Mttp , Hnf4a and ApoB in Isx − /− dams at 4 and 24 h post-maternal BC administration. Real-time qPCR analysis was performed in placenta of dams 4 h (A, upper panel) and 24 h (B, upper panel) post-maternal administration of BC. Values are calculated using the 2 − ΔΔCT method and expressed as mean ± SD with individual data points overlaid; n = 1 placenta/dam, from 2 to 4 dams/group. Immunoblot and quantification of placental MTP (97 kDa) (n = 1–2 placenta/dam, from 3 dams/group) at 4 and 24 h are shown in the middle and lower panels A and B, respectively. β-Actin (42 kDa) was used as the loading control. Values are expressed as mean ± SD. Statistical analysis by t-test ; *p ≤ 0.05 vs . the corresponding vehicle-treated group at each time point.
    Figure Legend Snippet: Placenta expression of Mttp , Hnf4a and ApoB in Isx − /− dams at 4 and 24 h post-maternal BC administration. Real-time qPCR analysis was performed in placenta of dams 4 h (A, upper panel) and 24 h (B, upper panel) post-maternal administration of BC. Values are calculated using the 2 − ΔΔCT method and expressed as mean ± SD with individual data points overlaid; n = 1 placenta/dam, from 2 to 4 dams/group. Immunoblot and quantification of placental MTP (97 kDa) (n = 1–2 placenta/dam, from 3 dams/group) at 4 and 24 h are shown in the middle and lower panels A and B, respectively. β-Actin (42 kDa) was used as the loading control. Values are expressed as mean ± SD. Statistical analysis by t-test ; *p ≤ 0.05 vs . the corresponding vehicle-treated group at each time point.

    Techniques Used: Expressing, Western Blot, Control

    Proposed model. In the early phase of this regulatory cascade, the expression of ISX is enhanced (likely by retinoic acid generated from BC) thus repressing HNF4α and resulting in attenuated expression of mRNA and protein levels of MTP. Later, ISX expression is attenuated (likely by β-apo-10′-carotenal generated from BC), thus increasing HNF4α and ultimately increasing expression of MTP at 24 h.
    Figure Legend Snippet: Proposed model. In the early phase of this regulatory cascade, the expression of ISX is enhanced (likely by retinoic acid generated from BC) thus repressing HNF4α and resulting in attenuated expression of mRNA and protein levels of MTP. Later, ISX expression is attenuated (likely by β-apo-10′-carotenal generated from BC), thus increasing HNF4α and ultimately increasing expression of MTP at 24 h.

    Techniques Used: Expressing, Generated



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    isx  (Bioss)
    94
    Bioss isx
    Placenta expression of Mttp , Hnf4a and ApoB in <t>Isx</t> − /− dams at 4 and 24 h post-maternal BC administration. Real-time qPCR analysis was performed in placenta of dams 4 h (A, upper panel) and 24 h (B, upper panel) post-maternal administration of BC. Values are calculated using the 2 − ΔΔCT method and expressed as mean ± SD with individual data points overlaid; n = 1 placenta/dam, from 2 to 4 dams/group. Immunoblot and quantification of <t>placental</t> <t>MTP</t> (97 kDa) (n = 1–2 placenta/dam, from 3 dams/group) at 4 and 24 h are shown in the middle and lower panels A and B, respectively. β-Actin (42 kDa) was used as the loading control. Values are expressed as mean ± SD. Statistical analysis by t-test ; *p ≤ 0.05 vs . the corresponding vehicle-treated group at each time point.
    Isx, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isx/product/Bioss
    Average 94 stars, based on 1 article reviews
    isx - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Placenta expression of Mttp , Hnf4a and ApoB in Isx − /− dams at 4 and 24 h post-maternal BC administration. Real-time qPCR analysis was performed in placenta of dams 4 h (A, upper panel) and 24 h (B, upper panel) post-maternal administration of BC. Values are calculated using the 2 − ΔΔCT method and expressed as mean ± SD with individual data points overlaid; n = 1 placenta/dam, from 2 to 4 dams/group. Immunoblot and quantification of placental MTP (97 kDa) (n = 1–2 placenta/dam, from 3 dams/group) at 4 and 24 h are shown in the middle and lower panels A and B, respectively. β-Actin (42 kDa) was used as the loading control. Values are expressed as mean ± SD. Statistical analysis by t-test ; *p ≤ 0.05 vs . the corresponding vehicle-treated group at each time point.

    Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids

    Article Title: The intestine-specific homeobox (ISX) modulates β-carotene-dependent regulation of microsomal triglyceride transfer protein (MTP) in a tissue-specific manner

    doi: 10.1016/j.bbalip.2024.159584

    Figure Lengend Snippet: Placenta expression of Mttp , Hnf4a and ApoB in Isx − /− dams at 4 and 24 h post-maternal BC administration. Real-time qPCR analysis was performed in placenta of dams 4 h (A, upper panel) and 24 h (B, upper panel) post-maternal administration of BC. Values are calculated using the 2 − ΔΔCT method and expressed as mean ± SD with individual data points overlaid; n = 1 placenta/dam, from 2 to 4 dams/group. Immunoblot and quantification of placental MTP (97 kDa) (n = 1–2 placenta/dam, from 3 dams/group) at 4 and 24 h are shown in the middle and lower panels A and B, respectively. β-Actin (42 kDa) was used as the loading control. Values are expressed as mean ± SD. Statistical analysis by t-test ; *p ≤ 0.05 vs . the corresponding vehicle-treated group at each time point.

    Article Snippet: Antibodies for MTP (cat.# BD 612022, BD Biosciences, Franklin Lakes, NJ) and ISX (cat.# BS-18172R, Bioss, Woburn, MA) were used at a dilution of 1:1000 and with an overnight incubation.

    Techniques: Expressing, Western Blot, Control

    Proposed model. In the early phase of this regulatory cascade, the expression of ISX is enhanced (likely by retinoic acid generated from BC) thus repressing HNF4α and resulting in attenuated expression of mRNA and protein levels of MTP. Later, ISX expression is attenuated (likely by β-apo-10′-carotenal generated from BC), thus increasing HNF4α and ultimately increasing expression of MTP at 24 h.

    Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids

    Article Title: The intestine-specific homeobox (ISX) modulates β-carotene-dependent regulation of microsomal triglyceride transfer protein (MTP) in a tissue-specific manner

    doi: 10.1016/j.bbalip.2024.159584

    Figure Lengend Snippet: Proposed model. In the early phase of this regulatory cascade, the expression of ISX is enhanced (likely by retinoic acid generated from BC) thus repressing HNF4α and resulting in attenuated expression of mRNA and protein levels of MTP. Later, ISX expression is attenuated (likely by β-apo-10′-carotenal generated from BC), thus increasing HNF4α and ultimately increasing expression of MTP at 24 h.

    Article Snippet: Antibodies for MTP (cat.# BD 612022, BD Biosciences, Franklin Lakes, NJ) and ISX (cat.# BS-18172R, Bioss, Woburn, MA) were used at a dilution of 1:1000 and with an overnight incubation.

    Techniques: Expressing, Generated