Journal: Oncotarget
Article Title: MiR-187 overexpression inhibits cervical cancer progression by targeting HPV16 E6
doi: 10.18632/oncotarget.17516
Figure Lengend Snippet: (A) HPV16 E6 mRNA levels detected in the presence of miR-187 mimics or miR-187 inhibitor (**P < 0.01, ***P <0.001). (B) Western blot detection of HPV16 E6 protein levels in CaSki cells in the presence of miR-187 mimics (***P <0.001). (C) Bioinformatics prediction of the miR-187 binding sites of the HPV16 E6 3′ UTR. The mutated HPV16 E6 3′ UTR is also shown. (D) Luciferase activity of CHO cells co-transfected with miR-187 expression vector or empty vector and HPV16 E6 3′ UTR reporter plasmid or its mutant form for 48 h. Data shown are the means ± SD of three replicates (**P < 0.01).
Article Snippet: Rabbit anti-human HPV16 E6 antibody (BS1719-R, 1:400) was purchased from BIOSS.
Techniques: Western Blot, Binding Assay, Luciferase, Activity Assay, Transfection, Expressing, Plasmid Preparation, Mutagenesis